# /usr/local/bin/fasta34_t -T 8 -b50 -d10 -E0.01 -H -O./tmp/as00072.fasta.nr -Q ../query/FLJ00072.ptfa /cdna2/lib/nr/nr 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00072, 204 aa vs /cdna2/lib/nr/nr library 2693465022 residues in 7827732 sequences statistics sampled from 60000 to 7818371 sequences Expectation_n fit: rho(ln(x))= 5.5507+/-0.000201; mu= 7.3973+/- 0.011 mean_var=110.7490+/-21.018, 0's: 50 Z-trim: 64 B-trim: 332 in 1/64 Lambda= 0.121872 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, open/ext: -10/-2, width: 16 The best scores are: opt bits E(7827732) gi|18676410|dbj|BAB84857.1| FLJ00072 protein [Homo ( 204) 1523 277.5 9.7e-73 gi|119584070|gb|EAW63666.1| PDZ and LIM domain 2 ( ( 166) 980 181.9 4.6e-44 gi|21748552|dbj|BAC03413.1| FLJ00349 protein [Homo ( 146) 604 115.7 3.4e-24 gi|108467919|gb|ABF93104.1| response regulator [My ( 420) 211 47.1 0.0044 >>gi|18676410|dbj|BAB84857.1| FLJ00072 protein [Homo sap (204 aa) initn: 1523 init1: 1523 opt: 1523 Z-score: 1462.1 bits: 277.5 E(): 9.7e-73 Smith-Waterman score: 1523; 100.000% identity (100.000% similar) in 204 aa overlap (1-204:1-204) 10 20 30 40 50 60 FLJ000 SPQCKRAACFLSAGSVGSEPGPSVQCLCTQIIACVTEDSVLSCHLLPSCCPVPGRTRMPP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|186 SPQCKRAACFLSAGSVGSEPGPSVQCLCTQIIACVTEDSVLSCHLLPSCCPVPGRTRMPP 10 20 30 40 50 60 70 80 90 100 110 120 FLJ000 SLSGPWQSLRPGSKGRGRGPLVGEGRGPPGVGPGNSRARPSWVCWPKALALTPPRLPPPN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|186 SLSGPWQSLRPGSKGRGRGPLVGEGRGPPGVGPGNSRARPSWVCWPKALALTPPRLPPPN 70 80 90 100 110 120 130 140 150 160 170 180 FLJ000 RPASAALATRRCWCCRLPRALVPPGPGTSRPRRVGLGSWKAGRGRRETGFPERDGVPQAQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|186 RPASAALATRRCWCCRLPRALVPPGPGTSRPRRVGLGSWKAGRGRRETGFPERDGVPQAQ 130 140 150 160 170 180 190 200 FLJ000 LAPPPPRFLPLPHALLLPPTPLLA :::::::::::::::::::::::: gi|186 LAPPPPRFLPLPHALLLPPTPLLA 190 200 >>gi|119584070|gb|EAW63666.1| PDZ and LIM domain 2 (myst (166 aa) initn: 958 init1: 958 opt: 980 Z-score: 947.2 bits: 181.9 E(): 4.6e-44 Smith-Waterman score: 980; 82.143% identity (86.905% similar) in 168 aa overlap (45-204:1-166) 20 30 40 50 60 FLJ000 SVGSEPGPSVQCLCTQIIACVTEDSVLSCHLLPSCCPVPGRTRMP----P----SLSGPW : :. .:: . : : ..:.:: gi|119 LSPAAFLLPGPRQNPHASLPLWTLAVSAPW 10 20 30 70 80 90 100 110 120 FLJ000 QSLRPGSKGRGRGPLVGEGRGPPGVGPGNSRARPSWVCWPKALALTPPRLPPPNRPASAA :: ..: :::::::::::::::::::::::::::::::::::::::::::::::: gi|119 --LRGKGQGARWGPLVGEGRGPPGVGPGNSRARPSWVCWPKALALTPPRLPPPNRPASAA 40 50 60 70 80 130 140 150 160 170 180 FLJ000 LATRRCWCCRLPRALVPPGPGTSRPRRVGLGSWKAGRGRRETGFPERDGVPQAQLAPPPP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|119 LATRRCWCCRLPRALVPPGPGTSRPRRVGLGSWKAGRGRRETGFPERDGVPQAQLAPPPP 90 100 110 120 130 140 190 200 FLJ000 RFLPLPHALLLPPTPLLA :::::::::::::::::: gi|119 RFLPLPHALLLPPTPLLA 150 160 >>gi|21748552|dbj|BAC03413.1| FLJ00349 protein [Homo sap (146 aa) initn: 615 init1: 588 opt: 604 Z-score: 590.6 bits: 115.7 E(): 3.4e-24 Smith-Waterman score: 604; 72.951% identity (77.049% similar) in 122 aa overlap (1-119:16-137) 10 20 30 40 FLJ000 SPQCKRAACFLSAGSVGSEPGPSVQCLCTQIIACVTEDSVLSCHL ::::::::::::::::::::::::::::::::::::::::::::: gi|217 LPGDFDPLLHPFMFLSPQCKRAACFLSAGSVGSEPGPSVQCLCTQIIACVTEDSVLSCHL 10 20 30 40 50 60 50 60 70 80 90 100 FLJ000 LPSCCPVPGRTRMPPSLSGPWQSLRPGSKGRGRGPLVGEGRGPPGVGPGNSRARPSWVCW ::::::::::::::::::::::::::::.:::::: .: : : . : : gi|217 LPSCCPVPGRTRMPPSLSGPWQSLRPGSEGRGRGPAGRRGLGAPDTLAPREGAGVRWSAR 70 80 90 100 110 120 110 120 130 140 150 160 FLJ000 ---PKALALTPPRLPPPNRPASAALATRRCWCCRLPRALVPPGPGTSRPRRVGLGSWKAG : . .: : :: gi|217 AGGPLVSGLGTAGLGPPGCVGPRLWP 130 140 >>gi|108467919|gb|ABF93104.1| response regulator [Myxoco (420 aa) initn: 161 init1: 99 opt: 211 Z-score: 211.6 bits: 47.1 E(): 0.0044 Smith-Waterman score: 211; 33.333% identity (46.667% similar) in 165 aa overlap (47-199:142-297) 20 30 40 50 60 70 FLJ000 GSEPGPSVQCLCTQIIACVTEDSVLSCHLLPSCCPVPGRTRMPPSLS-GPWQSLRPG--- :. :.: . ::. .: . ::: gi|108 LLDKVKALVGQKSNTMPASAATQVRHAAPQPAAAPAPVAAAAPPGARPAPPPGARPGVPP 120 130 140 150 160 170 80 90 100 110 120 FLJ000 SKGRGRGPLVGEGRGPPGV---GPGN--SRARPSWVCWPKALALTPPRLPPPNRPASAAL . : : : : : :::. ::: . ::: : : :: : :. : . : gi|108 GPGVPRPP--GAGVPPPGARPPGPGMPPGMARPPGPGMPPPGAPGAPRPPGPGMPPGMA- 180 190 200 210 220 130 140 150 160 170 180 FLJ000 ATRRCWCCRLPRALVPPGPGTSRPRRVGLGSWKAGRGRRETGFPERDGVPQAQLA-P--P : .: . ::::: : :. .: . :.: : :.: . .: : : gi|108 ---RPPGPGVPPGARPPGPGMPPGARPGVPPPPGGPA---PGLPPRPGMPPGAVARPGVP 230 240 250 260 270 280 190 200 FLJ000 PPRFLPLPHALLLPPTPLLA :: : : .. :: gi|108 PPPGGPAPGGFARPPVGAPQPPPGAAPQPAARGRDPFGLGAPAPAAAQPSISIEDSLPDQ 290 300 310 320 330 340 204 residues in 1 query sequences 2693465022 residues in 7827732 library sequences Tcomplib [34.26] (2 proc) start: Fri Feb 27 19:35:02 2009 done: Fri Feb 27 19:39:45 2009 Total Scan time: 673.470 Total Display time: 0.010 Function used was FASTA [version 34.26.5 April 26, 2007]