# /usr/local/bin/fasta34_t -T 4 -b50 -d10 -H -O./tmp/sh00463.fasta.huge -Q ../query/FLJ00351.ptfa ./tmplib.10216 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00351, 194 aa vs ./tmplib.10216 library 2210800 residues in 2457 sequences Expectation_n fit: rho(ln(x))= 4.2955+/-0.0041; mu= 16.3628+/- 0.277 mean_var=99.2248+/-22.076, 0's: 0 Z-trim: 1 B-trim: 0 in 0/40 Lambda= 0.128755 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, open/ext: -10/-2, width: 16 The best scores are: opt bits E(2457) KIAA1896 ( 568 res) fk06388 ( 568) 250 55.9 4e-09 KIAA0323 ( 724 res) hg00467 ( 724) 96 27.5 1.9 KIAA1986 ( 334 res) pg00636 ( 334) 87 25.3 3.9 KIAA0690 ( 1214 res) hk03594 (1214) 90 26.7 5.3 KIAA1358 ( 1123 res) fj02029 (1123) 88 26.3 6.6 KIAA0446 ( 351 res) fk03072 ( 351) 83 24.6 6.6 KIAA1852 ( 948 res) fj01514 ( 948) 87 26.0 6.9 FLJ00375 ( 708 res) sh07824 ( 708) 85 25.4 7.6 KIAA1662 ( 1653 res) fj03879s1 (1653) 88 26.5 8.2 KIAA0711 ( 661 res) hg00358 ( 661) 84 25.2 8.3 KIAA1691 ( 558 res) fj00597s1 ( 558) 83 24.9 8.6 KIAA0236 ( 1696 res) ha04654 (1696) 86 26.2 11 KIAA0050 ( 796 res) ha01557 ( 796) 82 24.9 12 KIAA1912 ( 589 res) fj18220 ( 589) 80 24.4 13 KIAA1740 ( 1119 res) pj00674 (1119) 81 25.0 16 KIAA1722 ( 1499 res) pf00674s1 (1499) 81 25.2 19 KIAA1375 ( 546 res) fj04426 ( 546) 76 23.6 21 KIAA0304 ( 2415 res) af07172 (2415) 82 25.6 22 KIAA1940 ( 821 res) ah04227 ( 821) 77 24.0 23 KIAA0171 ( 655 res) ha02502 ( 655) 76 23.7 23 KIAA1727 ( 1130 res) pg00153 (1130) 78 24.4 24 KIAA1045 ( 427 res) hh00656 ( 427) 73 22.9 27 KIAA1048 ( 897 res) hh02560 ( 897) 76 23.9 27 FLJ00372 ( 396 res) sh06918 ( 396) 72 22.6 29 KIAA0176 ( 265 res) ha03941 ( 265) 70 22.0 30 KIAA2038 ( 917 res) pj01991 ( 917) 75 23.7 31 FLJ00263 ( 764 res) sj01530 ( 764) 74 23.4 32 KIAA0910 ( 1315 res) hg02207s1 (1315) 76 24.1 34 KIAA1449 ( 680 res) fk04105 ( 680) 73 23.2 34 KIAA0843 ( 691 res) hk05155 ( 691) 73 23.2 35 KIAA0079 ( 1102 res) ha03543 (1102) 75 23.8 35 FLJ00043 ( 1415 res) as00043 (1415) 76 24.2 35 KIAA0365 ( 1181 res) hh00120s1 (1181) 75 23.9 36 KIAA1779 ( 1233 res) hg04229 (1233) 75 23.9 37 KIAA0072 ( 503 res) ha01357 ( 503) 71 22.6 38 KIAA0457 ( 845 res) hg00693 ( 845) 73 23.3 39 KIAA0361 ( 1371 res) hh00072 (1371) 75 24.0 39 FLJ00070 ( 1382 res) as00070 (1382) 75 24.0 39 FLJ00184 ( 580 res) sj01836 ( 580) 71 22.7 41 KIAA0734 ( 1190 res) hk03764s2 (1190) 74 23.7 41 FLJ00176 ( 403 res) sj00934 ( 403) 69 22.1 43 KIAA0574 ( 405 res) hj00204 ( 405) 69 22.1 43 KIAA0163 ( 550 res) ha01059 ( 550) 70 22.5 45 KIAA0183 ( 1111 res) ha02726 (1111) 73 23.5 45 KIAA0269 ( 567 res) ha06751 ( 567) 70 22.5 46 KIAA0150 ( 944 res) ha01348 ( 944) 72 23.2 47 KIAA1684 ( 762 res) fh24594 ( 762) 71 22.9 47 KIAA1545 ( 968 res) fj14026s1 ( 968) 72 23.2 47 FLJ00193 ( 491 res) sj02629 ( 491) 69 22.2 48 KIAA0425 ( 1270 res) hh01272 (1270) 73 23.6 48 >>KIAA1896 ( 568 res) fk06388 (568 aa) initn: 330 init1: 137 opt: 250 Z-score: 257.2 bits: 55.9 E(): 4e-09 Smith-Waterman score: 250; 30.636% identity (57.803% similar) in 173 aa overlap (22-193:302-463) 10 20 30 40 50 FLJ003 GASSQPKAPPGALMFHHQQAYCLAPFDLIKVRLQNQTEPRAQPGSPPPRYQ : ::.: .:: .: .. :.. . KIAA18 PDEFTVEERQTGMWWRHLVAGGGAGAVSRTCTAPLDRLKVLMQVHAS-RSNN-------M 280 290 300 310 320 60 70 80 90 100 110 FLJ003 GPVHCAASIFREEGPRGLFRGAWALTLRDTPTVGIYFITYEGLCRQYTPEGQNPSSATVL : : ....:: : :.:.:: .:. .: .: :..:: . : . .. : KIAA18 GIVGGFTQMIREGGARSLWRGNGINVLKIAPESAIKFMAYEQIKRLVGSDQETLRIHERL 330 340 350 360 370 380 120 130 140 150 160 170 FLJ003 VAGGFAGIASWVAATPLDVIKSRMQMDGLRRR-VYQGMLDCMVSSIRQEGLGVFFRGVTI :::..:: . . :..:.:.:: .::. :.::::: . .::...:..: . KIAA18 VAGSLAGAIAQSSIYPMEVLKTRM---ALRKTGQYSGMLDCARRILAREGVAAFYKGYVP 390 400 410 420 430 440 180 190 FLJ003 NSARAFPVNAVTFLSYEYLLRWWG : .: .. . :: : : KIAA18 NMLGIIPYAGIDLAVYETLKNAWLQHYAVNSADPGVFVLLACGTMSSTCGQLASYPLALV 450 460 470 480 490 500 >>KIAA0323 ( 724 res) hg00467 (724 aa) initn: 68 init1: 68 opt: 96 Z-score: 101.6 bits: 27.5 E(): 1.9 Smith-Waterman score: 96; 33.871% identity (51.613% similar) in 62 aa overlap (7-68:369-426) 10 20 30 FLJ003 GASSQPKAPPGALMFHHQQAYCLAPFDLIKVRLQNQ . :::: :. : . : ... : KIAA03 GGGARESAPLKGKALGKEEIALGGGGFCVHREPPGAHGSCHRAAQSRGASLLQRLHNGNA 340 350 360 370 380 390 40 50 60 70 80 90 FLJ003 TEPRAQPGSPPPRYQGPVHCAASIFREEGPRGLFRGAWALTLRDTPTVGIYFITYEGLCR . ::. : :::: . : ::. . : :: KIAA03 SPPRV-P-SPPPAPEPPWHCGDR--GDCGDRGDVGDRGDKQQGMARGRGPQWKRGARGGN 400 410 420 430 440 450 100 110 120 130 140 150 FLJ003 QYTPEGQNPSSATVLVAGGFAGIASWVAATPLDVIKSRMQMDGLRRRVYQGMLDCMVSSI KIAA03 LVTGTQRFKEALQDPFTLCLANVPGQPDLRHIVIDGSNVAMVHGLQHYFSSRGIAIAVQY 460 470 480 490 500 510 >>KIAA1986 ( 334 res) pg00636 (334 aa) initn: 44 init1: 44 opt: 87 Z-score: 95.9 bits: 25.3 E(): 3.9 Smith-Waterman score: 87; 31.884% identity (56.522% similar) in 69 aa overlap (33-92:237-302) 10 20 30 40 50 FLJ003 SSQPKAPPGALMFHHQQAYCLAPFDLIKVRLQNQTEPR---AQPGSPPPRYQGP-VHCAA : : .:. : : ::: :: :. .. KIAA19 SLPPAGAIDTRDTGSSEPQPPPEPPRSGPPLPNGLKPEFALALPPEPPP---GPEVKGGS 210 220 230 240 250 260 60 70 80 90 100 110 FLJ003 SIFR--EEGPRGLFRGAWALTL---RDTPTVGIYFITYEGLCRQYTPEGQNPSSATVLVA .. :.:::. .: :.: . . :.::. : .. KIAA19 CGLEHGERGPRAGVEGPWVLYVTPGKPLPVVGLSFPLFKMVLAGASKVGLSSHLHPASLG 270 280 290 300 310 320 120 130 140 150 160 170 FLJ003 GGFAGIASWVAATPLDVIKSRMQMDGLRRRVYQGMLDCMVSSIRQEGLGVFFRGVTINSA KIAA19 SWELFLGWGGM 330 >>KIAA0690 ( 1214 res) hk03594 (1214 aa) initn: 74 init1: 47 opt: 90 Z-score: 93.3 bits: 26.7 E(): 5.3 Smith-Waterman score: 90; 28.571% identity (57.143% similar) in 91 aa overlap (13-100:341-424) 10 20 30 40 FLJ003 GASSQPKAPPGALMFHHQQAYCLAPFDLIKVRLQNQTEPR-A :. :.:. : .: .. :.. . :. : KIAA06 MEKAHINLVRLQWDLGLGHLPRFFGTAVTCLLSPHSQVLTAATQSLKEI-LKECVAPHMA 320 330 340 350 360 50 60 70 80 90 FLJ003 QPGSPPPRYQGPVHCAASIFR--EEGPRGLFRGAWALTLRDTPTVGIYFITYEGLCRQYT . :: .::.. .:..:: ::: :..::. .:. . . .:. :: KIAA06 DIGSVTSSASGPAQSVAKMFRAVEEGLTYKFHAAWSSVLQ------LLCVFFEACGRQAH 370 380 390 400 410 420 100 110 120 130 140 150 FLJ003 PEGQNPSSATVLVAGGFAGIASWVAATPLDVIKSRMQMDGLRRRVYQGMLDCMVSSIRQE : KIAA06 PVMRKCLQSLCDLRLSPHFPHTAALDQAVGAAVTSMGPEVVLQAVPLEIDGSEETLDFPR 430 440 450 460 470 480 >>KIAA1358 ( 1123 res) fj02029 (1123 aa) initn: 52 init1: 52 opt: 88 Z-score: 91.6 bits: 26.3 E(): 6.6 Smith-Waterman score: 88; 35.088% identity (50.877% similar) in 57 aa overlap (5-56:1072-1123) 10 20 30 FLJ003 GASSQPKAPPGALMFHHQQAYCLAP-FDLIKVRL ::. : : : : .. .: :.:: KIAA13 GPLDQVWVIANKVQGSHSLSRGTVCHRTGVQPHEPKG-----HGWDYGIGGGWDHISVRA 1050 1060 1070 1080 1090 40 50 60 70 80 FLJ003 QNQTEPRA----QPGSPPPRYQGPVHCAASIFREEGPRGLFRGAWALTLRDTPTVGIYFI . ::. : : ::. .::: : KIAA13 NATRAPRSSSQEQEPSAPPEAHGPVCC 1100 1110 1120 >>KIAA0446 ( 351 res) fk03072 (351 aa) initn: 142 init1: 81 opt: 83 Z-score: 91.6 bits: 24.6 E(): 6.6 Smith-Waterman score: 169; 27.222% identity (53.889% similar) in 180 aa overlap (25-188:66-235) 10 20 30 40 50 FLJ003 GASSQPKAPPGALMFHHQQAYCLAPFDLIKVRLQNQTEPRAQPGSPPPRYQGPV :: ::..::: .: :. :.: KIAA04 IQIIEWEHLDKKKFYVFGVAMTMMIRVSVYPFTLIRTRLQ------VQKGKS--LYHGTF 40 50 60 70 80 60 70 80 90 100 110 FLJ003 HCAASIFREEGPRGLFRGAWALTLRDTPTVGIYFITYEGLCRQYTPEGQNPSSATVLVAG .:.: .: ::.:: . :. : : ..: : :... . .. ... :::: KIAA04 DAFIKILRADGITGLYRGFLVNTF--TLISGQCYVTTYELTRKFVADYSQSNTVKSLVAG 90 100 110 120 130 140 120 130 140 150 FLJ003 GFAGIASWVAATPLDVIKSR--MQMDGLRRRVYQ-------------GMLDCMVSSIRQ- : :.... ..:.::.... :: : . .: :. .. .: : KIAA04 GSASLVAQSITVPIDVVSQHLMMQRKGEKMGRFQVRGNPEGQGVVAFGQTKDIIRQILQA 150 160 170 180 190 200 160 170 180 190 FLJ003 EGLGVFFRGVTINSARAFPVNAVTFLSYEYLLRWWG .:: :.:: . . .: .:: . :.. KIAA04 DGLRGFYRGYVASLLTYIPNSAVWWPFYHFYAAIVFPWIPEQLSYLCPKECPHIVFQAVS 210 220 230 240 250 260 >>KIAA1852 ( 948 res) fj01514 (948 aa) initn: 98 init1: 52 opt: 87 Z-score: 91.4 bits: 26.0 E(): 6.9 Smith-Waterman score: 87; 33.333% identity (51.515% similar) in 99 aa overlap (36-126:80-170) 10 20 30 40 50 60 FLJ003 PKAPPGALMFHHQQAYCLAPFDLIKVRLQNQTEPRAQPGSPPPRYQGPVHC--AASIFRE :. :.:: : .:: : .:. :. KIAA18 YPGLFAVGGDGGGGWSGTGTTKSILPPASTQVPERSQPRPGRARRRGPGTCHGGAAARRR 50 60 70 80 90 100 70 80 90 100 110 FLJ003 EGPRGLF-----RGAWALTLRDTPTVGIYFITYE-GLCRQYTPEGQNPSSATVLVAGGFA . : : ::: ::.:: . . : :.: .: :: :.. .:: ... KIAA18 KCPPGTTAPSRPRGA----LRSTPGKQPPLSAAEVGIC--CVP-GQVPGQ-SVLGMAAIN 110 120 130 140 150 160 120 130 140 150 160 170 FLJ003 GIASWVAATPLDVIKSRMQMDGLRRRVYQGMLDCMVSSIRQEGLGVFFRGVTINSARAFP ::: : : KIAA18 PWASWGALTDQSWGMTAVDPWASWALCPQYPAWHVEGSLEEGRRATGLPAAQVQEPVTFK 170 180 190 200 210 220 >>FLJ00375 ( 708 res) sh07824 (708 aa) initn: 55 init1: 55 opt: 85 Z-score: 90.6 bits: 25.4 E(): 7.6 Smith-Waterman score: 85; 27.451% identity (43.137% similar) in 102 aa overlap (2-88:383-477) 10 20 FLJ003 GASSQPKAPPGAL----MFHHQQAYCLA--P :. .: :::. . .. :: :. : FLJ003 RAPGLARAPGPLAPAPAGEGDREYVSPDWAAAPEPAAPPAEIPYEELWAHQGPEGLVRPP 360 370 380 390 400 410 30 40 50 60 70 FLJ003 FDLIKVRLQNQTEPRAQPGSPPPRYQGPVHCAASIFREE---------GPRGLFRGAWAL : . . :: .: .::: :: . .:: ::: : . FLJ003 PGLDLISFGAAGPPRREPEAPPP----PVPPKSEAVKEECRLLNAPPVPPRG---GNGSG 420 430 440 450 460 80 90 100 110 120 130 FLJ003 TLRDTPTVGIYFITYEGLCRQYTPEGQNPSSATVLVAGGFAGIASWVAATPLDVIKSRMQ : ..: : : FLJ003 RLSSSPPVPPRFPKLQPVHSPSSSLSYYSSGLQDGAGSRSGSGSPSPDTYSLYCYPCTWG 470 480 490 500 510 520 >>KIAA1662 ( 1653 res) fj03879s1 (1653 aa) initn: 81 init1: 57 opt: 88 Z-score: 90.0 bits: 26.5 E(): 8.2 Smith-Waterman score: 88; 32.353% identity (57.353% similar) in 68 aa overlap (3-67:294-360) 10 20 30 FLJ003 GASSQPKAPPGALMFHHQQA--YCLAPFDLIK : .:. :: :. . :..: : : . KIAA16 ATSSSHNPGHQSTSRTSSPVYPAAYGAPLTSPEPSQPPCAVCIGHRDAPRASSPPRYLQH 270 280 290 300 310 320 40 50 60 70 80 FLJ003 VRLQNQTEPRAQPGSPPPRYQGP-VHCAASIFREEGPRGLFRGAWALTLRDTPTVGIYFI . :::: : : ::... : . :. : ....:: KIAA16 DPFPFFPEPRA-PESEPPHHEPPYIPPAVCIGHRDAPRASSPPRHTQFDPFPFLPDTSDA 330 340 350 360 370 380 90 100 110 120 130 140 FLJ003 TYEGLCRQYTPEGQNPSSATVLVAGGFAGIASWVAATPLDVIKSRMQMDGLRRRVYQGML KIAA16 EHQCQSPQHEPLQLPAPVCIGYRDAPRASSPPRQAPEPSLLFQDLPRASTESLVPSMDSL 390 400 410 420 430 440 >>KIAA0711 ( 661 res) hg00358 (661 aa) initn: 37 init1: 37 opt: 84 Z-score: 89.9 bits: 25.2 E(): 8.3 Smith-Waterman score: 84; 24.409% identity (49.606% similar) in 127 aa overlap (37-159:348-467) 10 20 30 40 50 60 FLJ003 KAPPGALMFHHQQAYCLAPFDLIKVRLQNQTEPRAQPGSPPPRYQGPVHC---AASIFRE ..:.. :. : .. :.: ::. .:: KIAA07 ERDLLLRRRLRAGRAHLLAAALGPAGERAGSRPQSPSGDADARGDAAVYCFHAAAGEWRE 320 330 340 350 360 370 70 80 90 100 110 120 FLJ003 EGPRGLFRGAWALTLRDTPTVGIY-FITYEGLCRQYTPEGQNPSSATVLVAGGFAGIASW : .:: : : .: .. : :.:. : :. . . :: KIAA07 L--TRLPEGAPA---RGCGLCVLYNYLFVAGGVAPAGPDGRARPSDQVFCYN--PATDSW 380 390 400 410 420 430 130 140 150 160 170 180 FLJ003 VAATPLDVIKSRMQMDGLRRRVYQGMLDCMVSSIRQEGLGVFFRGVTINSARAFPVNAVT :. :: .:.... .: ..: .:..: : . KIAA07 SAVRPLRQARSQLRLLALDGHLYAVGGECLLSVERYDPRADRWAPVAPLPRGAFAVAHEA 440 450 460 470 480 490 190 FLJ003 FLSYEYLLRWWG KIAA07 TTCHGEIYVSGGSLFYRLLKYDPRRDEWQECPCSSSRERSADMVALDGFIYRFDLSGSRG 500 510 520 530 540 550 194 residues in 1 query sequences 2210800 residues in 2457 library sequences Tcomplib [34.26] (2 proc) start: Fri Feb 27 11:01:40 2009 done: Fri Feb 27 11:01:40 2009 Total Scan time: 0.360 Total Display time: 0.040 Function used was FASTA [version 34.26.5 April 26, 2007]