# /usr/local/bin/fasta34_t -T 4 -b50 -d10 -H -O./tmp/sh04156.fasta.huge -Q ../query/FLJ00300.ptfa ./tmplib.10216 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00300, 283 aa vs ./tmplib.10216 library 2210711 residues in 2457 sequences Expectation_n fit: rho(ln(x))= 4.1049+/-0.0034; mu= 14.1778+/- 0.229 mean_var=75.3917+/-16.865, 0's: 0 Z-trim: 6 B-trim: 0 in 0/40 Lambda= 0.147711 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, open/ext: -10/-2, width: 16 The best scores are: opt bits E(2457) KIAA1158 ( 230 res) hj00081 ( 230) 131 37.0 0.0012 KIAA1496 ( 920 res) fj09513 ( 920) 112 33.7 0.046 KIAA0230 ( 1496 res) ha02538 (1496) 104 32.3 0.2 KIAA1233 ( 1023 res) fh08611 (1023) 98 30.8 0.39 KIAA0756 ( 1222 res) ff01911 (1222) 98 30.9 0.43 FLJ00259 ( 659 res) sh04102 ( 659) 95 29.9 0.46 KIAA1556 ( 1595 res) fh18619 (1595) 95 30.4 0.79 KIAA1132 ( 2092 res) hh01132s1 (2092) 95 30.5 0.93 KIAA1290 ( 1011 res) hj00086s2 (1011) 92 29.5 0.93 KIAA1851 ( 523 res) hg04492 ( 523) 87 28.1 1.3 KIAA1436 ( 924 res) hh15451 ( 924) 88 28.6 1.6 FLJ00271 ( 211 res) sh00109 ( 211) 80 26.0 2.1 FLJ00014 ( 725 res) as00014 ( 725) 85 27.8 2.1 KIAA1163 ( 437 res) hj05329 ( 437) 81 26.7 2.8 KIAA1777 ( 954 res) fh19201x1 ( 954) 82 27.3 3.9 KIAA0420 ( 756 res) fg02987 ( 756) 80 26.8 4.6 KIAA0115 ( 456 res) ha00643 ( 456) 75 25.4 7.1 KIAA0657 ( 1237 res) hk01859s1 (1237) 78 26.6 8.3 KIAA1069 ( 787 res) hj05486 ( 787) 76 25.9 8.5 KIAA0331 ( 814 res) hg00928 ( 814) 76 26.0 8.7 KIAA1561 ( 1416 res) fh20178s1 (1416) 78 26.7 9 FLJ00236 ( 702 res) sj07289 ( 702) 75 25.7 9.2 FLJ00411 ( 437 res) sf00010 ( 437) 73 25.0 9.3 KIAA1083 ( 584 res) hj07423 ( 584) 74 25.3 9.5 KIAA0088 ( 943 res) ha01225 ( 943) 75 25.8 11 FLJ00074 ( 191 res) as00074 ( 191) 68 23.4 12 KIAA1876 ( 803 res) pf01162s1 ( 803) 73 25.3 13 KIAA1837 ( 639 res) fh22040s1 ( 639) 72 25.0 14 KIAA1263 ( 850 res) hj00608 ( 850) 73 25.3 14 KIAA1486 ( 677 res) fj07162 ( 677) 72 25.0 14 KIAA0364 ( 1437 res) hh00116 (1437) 74 25.9 16 KIAA1504 ( 447 res) hk04058 ( 447) 69 24.1 17 KIAA1779 ( 1233 res) hg04229 (1233) 73 25.6 17 KIAA1437 ( 811 res) hk07206 ( 811) 71 24.9 18 KIAA1580 ( 640 res) fj04979 ( 640) 70 24.5 18 FLJ00060 ( 227 res) as00060 ( 227) 65 22.9 20 KIAA1756 ( 1001 res) fh13904(revised) (1001) 71 25.0 21 FLJ00138 ( 309 res) sh03216 ( 309) 66 23.3 21 KIAA1568 ( 1380 res) fh22308 (1380) 72 25.4 22 KIAA0987 ( 1115 res) hk01713 (1115) 71 25.1 22 KIAA1989 ( 1097 res) fh00385 (1097) 70 24.8 25 KIAA1911 ( 864 res) fj02022 ( 864) 69 24.5 25 KIAA0874 ( 601 res) hk07302 ( 601) 67 23.9 27 KIAA0543 ( 1111 res) hg04390 (1111) 69 24.6 29 KIAA1824 ( 958 res) fh17057 ( 958) 68 24.3 31 KIAA1514 ( 1598 res) fh00815(revised) (1598) 70 25.1 32 KIAA0855 ( 632 res) hk06219 ( 632) 66 23.7 32 KIAA0482 ( 1332 res) hj01880 (1332) 69 24.7 33 KIAA1926 ( 412 res) hh10052 ( 412) 64 23.0 34 KIAA0025 ( 422 res) ha00594 ( 422) 64 23.0 34 >>KIAA1158 ( 230 res) hj00081 (230 aa) initn: 100 init1: 72 opt: 131 Z-score: 158.8 bits: 37.0 E(): 0.0012 Smith-Waterman score: 131; 26.549% identity (61.062% similar) in 113 aa overlap (150-260:31-137) 120 130 140 150 160 170 FLJ003 CNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGC .. .: . : : :.: . :. ... : KIAA11 RSWGGESLGSRQPQKMPAFNRLFPLASLVLIYWVSVCFPVCVEVPSETEAVQGNPMKLRC 10 20 30 40 50 60 180 190 200 210 220 230 FLJ003 V--FQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIF . .. ::. .: :::.. . .:. ....: . .. :: . ::.:.. :. KIAA11 ISCMKREEVEATTVVEWFYRPEGGKDFLIYEYRNG-HQEVE-----SPFQGRLQWNGSKD 70 80 90 100 110 240 250 260 270 280 FLJ003 RNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRSI .: :: . .: .:.: :::.. KIAA11 LQDVSITVLNVTLNDSGLYTCNVSREFEFEAHRPFVKTTRLIPLRVTEEAGEDFTSVVSE 120 130 140 150 160 170 >>KIAA1496 ( 920 res) fj09513 (920 aa) initn: 81 init1: 81 opt: 112 Z-score: 130.4 bits: 33.7 E(): 0.046 Smith-Waterman score: 148; 22.485% identity (57.988% similar) in 169 aa overlap (119-283:350-496) 90 100 110 120 130 140 FLJ003 AKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGES : :::.: . .: .:: ::: : .:. KIAA14 SLDCKPRASPRALSSWKKGDVSVQEHERISLLNDGGLKIANVTKADAGTYTCM----AEN 320 330 340 350 360 370 150 160 170 180 190 200 FLJ003 QVFKKAVVLHVLPEEPKELMVHVGGL-IQMG-CVFQSTEVKHVTKVEWIFSGRRAKEEIV : : . :.. :: .. . ... ...: :. .:.: .. :. KIAA14 QFGKANGTTHLVVTEPTRITLAPSNMDVSVGESVILPCQVQHDPLLD-----------II 380 390 400 410 420 210 220 230 240 250 260 FLJ003 FRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLG--N : .: . .. ..... .::.. :: ..:..:..... . .:.:.: .. : . KIAA14 FTWYFNGALA-DFKKDGSHFEK----VGG--SSSGDLMIRNIQLKHSGKYVCMVQTGVDS 430 440 450 460 470 270 280 FLJ003 LVFKKTIVLHVSPEEPRSI . .... :: :... KIAA14 VSSAADLIVRGSPGPPENVKVDEITDTTAQLSWKEGKDNHSPVISYSIQARTPFSVGWQT 480 490 500 510 520 530 >>KIAA0230 ( 1496 res) ha02538 (1496 aa) initn: 139 init1: 90 opt: 104 Z-score: 118.9 bits: 32.3 E(): 0.2 Smith-Waterman score: 110; 24.667% identity (51.333% similar) in 150 aa overlap (119-259:313-430) 90 100 110 120 130 140 FLJ003 AKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIR-LKGE : .::.:..:..::.::: : : . . :: KIAA02 TCRAEGNPKPEIIWLRNNNELSMKTDSRLNLLDDGTLMIQNTQETDQGIYQCMAKNVAGE 290 300 310 320 330 340 150 160 170 180 190 FLJ003 SQVFKKAVVLHVL--PE------EPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGR .. . :.:. . : .:.. : :: . . : :. . .. : : KIAA02 VKT--QEVTLRYFGSPARPTFVIQPQNTEVLVGESVTLEC---SATGHPPPRISWT-RGD 350 360 370 380 390 200 210 220 230 240 250 FLJ003 RAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCS :. . . :::.. . :....:.: ..:.:.:.:: KIAA02 RTPLPV---------------------DPRVNITPS-----GGLYIQNVVQGDSGEYACS 400 410 420 430 260 270 280 FLJ003 IHLGNLVFKKTIVLHVSPEEPRSI KIAA02 ATNNIDSVHATAFIIVQALPQFTVTPQDRVVIEGQTVDFQCEAKGNPPPVIAWTKGGSQL 440 450 460 470 480 490 >>KIAA1233 ( 1023 res) fh08611 (1023 aa) initn: 102 init1: 80 opt: 98 Z-score: 113.8 bits: 30.8 E(): 0.39 Smith-Waterman score: 98; 32.911% identity (65.823% similar) in 79 aa overlap (114-187:672-743) 90 100 110 120 130 140 FLJ003 SPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDI-LCNDGSLLLQDVQEADQGTYICEI : :.. : .::::::.:. ..:::.: KIAA12 EAALGANVTIRCPVKGVPQPNITWLKRGGSLSGNVSLLFNGSLLLQNVSLENEGTYVC-- 650 660 670 680 690 150 160 170 180 190 FLJ003 RLKGESQVFKKAV---VLHVLPEE-PKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSG .... ::: :::.: .. :. .: . : .. ..:.:... KIAA12 ---IATNALGKAVATSVLHLLERRWPESRIVFLQGHKKY--ILQATNTRTNSNDPTGEPP 700 710 720 730 740 750 200 210 220 230 240 250 FLJ003 RRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTC KIAA12 PQEPFWEPGNWSHCSATCGHLGARIQRPQCVMANGQEVSEALCDHLQKPLAGFEPCNIRD 760 770 780 790 800 810 >>KIAA0756 ( 1222 res) ff01911 (1222 aa) initn: 133 init1: 77 opt: 98 Z-score: 112.9 bits: 30.9 E(): 0.43 Smith-Waterman score: 120; 24.706% identity (50.588% similar) in 170 aa overlap (122-283:547-683) 100 110 120 130 140 FLJ003 EYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYIC---EIRLKGES .::: .. ... ::: : : .: :.:. KIAA07 PFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAEN 520 530 540 550 560 570 150 160 170 180 190 200 FLJ003 QV---FKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEI :: : . .. .::. ... : .:. : .::: ... : . : . KIAA07 QVRLEVKDPTRIYRMPEDQ---VARRGTTVQLEC-----RVKHDPSLKLTVSWLKDDEPL 580 590 600 610 620 210 220 230 240 250 260 FLJ003 VFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTC--SIHLG .. ::.. ..: :. . :: : : :.::: : .: KIAA07 -------------------YIGNRMK------KEDDSLTIFGVAERDQGSYTCVASTELD 630 640 650 660 270 280 FLJ003 NLVFKKTIVLHVSPEEPRSI . . : ... :..::.. KIAA07 QDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGV 670 680 690 700 710 720 >>FLJ00259 ( 659 res) sh04102 (659 aa) initn: 201 init1: 87 opt: 95 Z-score: 112.4 bits: 29.9 E(): 0.46 Smith-Waterman score: 118; 27.811% identity (48.521% similar) in 169 aa overlap (122-281:475-610) 100 110 120 130 140 150 FLJ003 EYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVF ::::... : : : : : . ..:... FLJ002 TCRAEGFPPPAIEWQRDGQPVSSPRHQLQPDGSLVISRVAVEDGGFYTC-VAFNGQDRD- 450 460 470 480 490 500 160 170 180 190 200 FLJ003 KKAVVLHVLPEE-----PKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIV .. : :.:: : : . : : .. :: . : ...: .: FLJ002 QRWVQLRVLGELTISGLPPTVTVPEGDTARLLCVVAGESV----NIRWSRNG-------- 510 520 530 540 550 210 220 230 240 250 260 FLJ003 FRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNL- . : :. :: ::. ::.... ..: : :.:::: . :. FLJ002 --------LPV---QADGH---RVHQ-----SPDGTLLIYNLRARDEGSYTCSAYQGSQA 560 570 580 590 270 280 FLJ003 VFKKTIVLHVSPE---EPRSI : ..: : ::: .:: FLJ002 VSRSTEVKVVSPAPTAQPRDPGRDCVDQPELANCDLILQAQLCGNEYYSSFCCASCSRFQ 600 610 620 630 640 650 >>KIAA1556 ( 1595 res) fh18619 (1595 aa) initn: 70 init1: 70 opt: 95 Z-score: 108.2 bits: 30.4 E(): 0.79 Smith-Waterman score: 102; 22.667% identity (48.889% similar) in 225 aa overlap (71-279:367-550) 50 60 70 80 90 FLJ003 SLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFK---IDWTLSPGEHAKDEYVLYY :: .:. : :.. :: : .. KIAA15 VLTLRQLALKDSGTIYFEAGDQRASAALRVTEKPSVFSRELTDATITEGE---DLTLVCE 340 350 360 370 380 390 100 110 120 130 140 150 FLJ003 YSNLSVPIGRFQNRVHLMGDILC---NDG---SLLLQDVQEADQGTYICEIRLKGESQVF :. ..:. .. : :. : ..: .::. . :.: : :: :.. KIAA15 TSTCDIPVCWTKDGKTLRGSARCQLSHEGHRAQLLITGATLQDSGRYKCEAGGACSSSI- 400 410 420 430 440 450 160 170 180 190 200 FLJ003 KKAVVLHVLP----EEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVF : .:. : : :.: : :: . ::..:. .. :.: ... KIAA15 ---VRVHARPVRFQEALKDLEVLEGGAATLRCVLSSV----AAPVKWCYGN--------- 460 470 480 490 210 220 230 240 250 260 FLJ003 RYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIM---LQGVRESDGGNYTCSIHLGN . :: . .:: .:..:... ....: .:.: :.:: .:. KIAA15 ---NVLRPGDKYS----------------LRQEGAMLELVVRNLRPQDSGRYSCS--FGD 500 510 520 530 270 280 FLJ003 LVFKKTIVLHVSPEEPRSI . . :... . : . KIAA15 QTTSATLTVTALPAQFIGKLRNKEATEGATATLRCELSKAAPVEWRKGSETLRDGDRYCL 540 550 560 570 580 590 >>KIAA1132 ( 2092 res) hh01132s1 (2092 aa) initn: 76 init1: 49 opt: 95 Z-score: 106.9 bits: 30.5 E(): 0.93 Smith-Waterman score: 108; 25.581% identity (65.116% similar) in 86 aa overlap (118-199:591-674) 90 100 110 120 130 140 FLJ003 HAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEA-DQGTYICEIRLKG .. ..:.: : :::.. :.: :.: . .. KIAA11 TLINCRVIGYPYYSIKWYKDALLLPDNHRQVVFENGTLKLTDVQKGMDEGEYLCSVLIQP 570 580 590 600 610 620 150 160 170 180 190 200 FLJ003 ESQVFKKA-VVLHVLPE-EPKELM-VHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKE . .. ... :...: : .: :. . .: :. . :: .: .. .. : .:. KIAA11 QLSISQSVHVAVKVPPLIQPFEFPPASIGQLLYIPCVVSSGDMP--IRITWRKDGQVIIS 630 640 650 660 670 210 220 230 240 250 260 FLJ003 EIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLG KIAA11 GSGVTIESKEFMSSLQISSVSLKHNGNYTCIASNAAATVSRERQLIVRVPPRFVVQPNNQ 680 690 700 710 720 730 >>KIAA1290 ( 1011 res) hj00086s2 (1011 aa) initn: 41 init1: 41 opt: 92 Z-score: 106.9 bits: 29.5 E(): 0.93 Smith-Waterman score: 92; 24.286% identity (49.286% similar) in 140 aa overlap (11-144:576-708) 10 20 30 FLJ003 SCGEISVHCLPWV-LFILDLKVESSMFCPLKLILLPVLLD :: .: .: . .:: :: : : . KIAA12 EIAKYDRICEEAYGRSKDKKILHIKHWLDSPWPGFFNVDGE-PKSMTCPATGI--PEDML 550 560 570 580 590 600 40 50 60 70 80 90 FLJ003 YSLGLNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSP----GEHAKDEYVL .: .: .. .:.: : .. . :... .::.:. : : : . KIAA12 THIGSVASSVPLEDFKIHTGLSRILRGRADMTKNR---TVDWALAEYMAFGSLLK-EGIH 610 620 630 640 650 100 110 120 130 140 150 FLJ003 YYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTY-ICEIRLKGESQVFKKA :. .: : :..: :.. : . . . .. ::. : .:. : KIAA12 VRLSGQDVERGTFSHRHHVLHDQEVDRRTCVPMNHLWPDQAPYTVCNSSLSEYGVLGFEL 660 670 680 690 700 710 160 170 180 190 200 210 FLJ003 VVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLR KIAA12 GYAMASPNALVLWEAQFGDFHNTAQCIIDQFISTGQAKWVRHNGIVLLLPHGMEGMGPEH 720 730 740 750 760 770 >>KIAA1851 ( 523 res) hg04492 (523 aa) initn: 69 init1: 69 opt: 87 Z-score: 104.3 bits: 28.1 E(): 1.3 Smith-Waterman score: 90; 29.730% identity (49.550% similar) in 111 aa overlap (85-179:303-410) 60 70 80 90 100 FLJ003 TVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEH--AKDEYVLYYYSNLSVPIGRF---Q : :: : : : : ::: :. . KIAA18 FKVPASRVRFFQHSRVFENCSSAPALGLERPEEHLYALVGRSLRLYCNTSVPAMRIAWVS 280 290 300 310 320 330 110 120 130 140 150 FLJ003 NRVHLM-------GDI-LCNDGSLLLQDVQEADQGTYICEI---RLKGESQVFKKAVVLH . .:. :.: . :::: . .::: : ..: ::. ..:. . : .: KIAA18 PQQELLRAPGSRDGSIAVLADGSLAIGNVQEQHAGLFVCLATGPRLH-HNQTHEYNVSVH 340 350 360 370 380 390 160 170 180 190 200 210 FLJ003 VLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVE ::. . ..: .:: KIAA18 FPRPEPEAF--NTGFTTLLGCAVGLVLVLLYLFAPPCRCCRRACRCRRWPQTPSPLQELS 400 410 420 430 440 283 residues in 1 query sequences 2210711 residues in 2457 library sequences Tcomplib [34.26] (2 proc) start: Fri Feb 27 10:51:18 2009 done: Fri Feb 27 10:51:18 2009 Total Scan time: 0.430 Total Display time: 0.070 Function used was FASTA [version 34.26.5 April 26, 2007]