# /usr/local/bin/fasta34_t -T 4 -b50 -d10 -H -O./tmp/sh05037.fasta.huge -Q ../query/FLJ00304.ptfa ./tmplib.10216 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00304, 381 aa vs ./tmplib.10216 library 2210613 residues in 2457 sequences Expectation_n fit: rho(ln(x))= 5.4283+/-0.00459; mu= 13.0230+/- 0.308 mean_var=127.5006+/-28.297, 0's: 0 Z-trim: 4 B-trim: 8 in 1/39 Lambda= 0.113584 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 37, opt: 25, open/ext: -10/-2, width: 16 The best scores are: opt bits E(2457) KIAA1902 ( 1112 res) hj04383 (1112) 1174 203.5 5.6e-53 KIAA2014 ( 1032 res) ff00951s1 (1032) 1134 196.9 5e-51 KIAA0666 ( 1085 res) hk02075 (1085) 316 62.9 1.2e-10 KIAA0381 ( 1114 res) ah04695 (1114) 308 61.6 2.9e-10 FLJ00366 ( 282 res) sh05203 ( 282) 97 26.3 3.2 FLJ00232 ( 318 res) sj06640 ( 318) 95 26.0 4.4 KIAA2011 ( 1091 res) pj02017 (1091) 100 27.5 5.3 KIAA1629 ( 1329 res) fh20517 (1329) 100 27.6 6 FLJ00079 ( 84 res) as00079 ( 84) 84 23.5 6.7 FLJ00261 ( 1651 res) sh06537 (1651) 98 27.4 8.5 KIAA0685 ( 939 res) hk02959 ( 939) 94 26.5 9.5 FLJ00390 ( 322 res) sj04630 ( 322) 88 24.9 9.7 KIAA0437 ( 1605 res) hj00203s1 (1605) 96 27.1 11 KIAA1668 ( 791 res) fh11717 ( 791) 92 26.0 11 FLJ00034 ( 1343 res) as00034 (1343) 94 26.7 12 KIAA1076 ( 804 res) hj06779 ( 804) 91 25.9 12 KIAA1802 ( 821 res) fj21631 ( 821) 91 25.9 12 KIAA0904 ( 1535 res) ha00554 (1535) 94 26.7 13 KIAA1357 ( 836 res) fj01906 ( 836) 90 25.7 14 KIAA0333 ( 1223 res) pf04993 (1223) 92 26.3 14 KIAA0757 ( 534 res) hk04606 ( 534) 87 25.0 15 KIAA1486 ( 677 res) fj07162 ( 677) 88 25.3 15 KIAA1595 ( 471 res) fj09517 ( 471) 84 24.4 19 KIAA0360 ( 1019 res) hh00062s1 (1019) 87 25.4 22 KIAA0600 ( 1080 res) fh08981 (1080) 86 25.2 26 KIAA0859 ( 707 res) hk06485s1 ( 707) 83 24.5 28 KIAA0618 ( 1052 res) hg03971 (1052) 85 25.1 28 KIAA1524 ( 884 res) fj00637(revised) ( 884) 84 24.8 28 KIAA0865 ( 1900 res) hk06640y1 (1900) 88 25.9 29 KIAA1515 ( 757 res) fh04655(revised) ( 757) 83 24.5 29 KIAA0807 ( 1329 res) hk04449(revised) (1329) 86 25.3 29 KIAA0954 ( 1183 res) hj05543s1 (1183) 85 25.1 30 KIAA1810 ( 579 res) fk03376 ( 579) 81 24.1 31 KIAA0311 ( 2324 res) hg00112s1 (2324) 88 26.0 33 FLJ00356 ( 851 res) sh02495 ( 851) 82 24.4 35 KIAA1968 ( 860 res) fk04713 ( 860) 82 24.4 35 KIAA0612 ( 1810 res) hg00654s1 (1810) 86 25.5 35 FLJ00115 ( 757 res) as00115 ( 757) 81 24.2 36 KIAA1506 ( 3309 res) ff04330 (3309) 89 26.3 36 KIAA0656 ( 912 res) hk01789 ( 912) 82 24.5 36 KIAA0992 ( 772 res) hk07554 ( 772) 81 24.2 37 KIAA0461 ( 1355 res) hg00867 (1355) 84 25.0 37 KIAA2017 ( 3051 res) fk13965y1 (3051) 88 26.1 39 KIAA0938 ( 1257 res) hh04777s1 (1257) 83 24.8 39 FLJ00330 ( 246 res) sj03258 ( 246) 74 22.4 40 KIAA0662 ( 1114 res) hk01953s1 (1114) 82 24.6 41 KIAA0947 ( 2330 res) ef03552 (2330) 86 25.7 41 FLJ00287 ( 186 res) sh02562 ( 186) 72 22.0 42 KIAA1986 ( 334 res) pg00636 ( 334) 75 22.8 43 KIAA1681 ( 1236 res) fh23697 (1236) 82 24.6 44 >>KIAA1902 ( 1112 res) hj04383 (1112 aa) initn: 1151 init1: 1109 opt: 1174 Z-score: 1044.5 bits: 203.5 E(): 5.6e-53 Smith-Waterman score: 1174; 71.373% identity (89.020% similar) in 255 aa overlap (85-331:168-422) 60 70 80 90 100 110 FLJ003 AGLSLLAFPLPPLKWPLQCWPPAPLHPVPRYDMESTDNGASNS-EKNKPLEQSVEDLSKG .:.::... . .: .:.:: .:.::: .: KIAA19 IGWVREFLNEENKGLDVLVEYLSFAQYAVTFDFESVESTVESSVDKSKPWSRSIEDLHRG 140 150 160 170 180 190 120 130 140 150 160 FLJ003 P--PSSVPKS-----RHLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGF :: : .: :: ... . ::..:.:::.::.:::::::::::::::::: :: KIAA19 SNLPSPVGNSVSRSGRHSALRYNTLPSRRTLKNSRLVSKKDDVHVCIMCLRAIMNYQYGF 200 210 220 230 240 250 170 180 190 200 210 220 FLJ003 SLVMNHPACVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQH ..::.:: :::::::::::::::::::::::::::::::::.:::.:::::::::::.. KIAA19 NMVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHEIILSAFDNFKEVCGEKQ 260 270 280 290 300 310 230 240 250 260 270 280 FLJ003 RFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLERLRL :::::::.:::::.::::::: ::::::::::::.::::: ::::::.:::: ::..:. KIAA19 RFEKLMEHFRNEDNNIDFMVASMQFINIVVHSVEDMNFRVHLQYEFTKLGLDEYLDKLKH 320 330 340 350 360 370 290 300 310 320 330 340 FLJ003 TESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALVRVGPDPSPAHHRP ::::::::::::::::.:::::::::.:::::.::..:::.:... KIAA19 TESDKLQVQIQAYLDNVFDVGALLEDAETKNAALERVEELEENISHLSEKLQDTENEAMS 380 390 400 410 420 430 350 360 370 380 FLJ003 TGHTGHRATQAWALGSRQCYNCALEVLPAWPQSLA KIAA19 KIVELEKQLMQRNKELDVVREIYKDANTQVHTLRKMVKEKEEAIQRQSTLEKKIHELEKQ 440 450 460 470 480 490 >>KIAA2014 ( 1032 res) ff00951s1 (1032 aa) initn: 1110 init1: 1084 opt: 1134 Z-score: 1009.4 bits: 196.9 E(): 5e-51 Smith-Waterman score: 1139; 71.260% identity (87.008% similar) in 254 aa overlap (87-331:162-411) 60 70 80 90 100 110 FLJ003 LSLLAFPLPPLKWPLQCWPPAPLHPVPRYDMESTDNGASNSEKNKPLEQSVEDLSKGPPS .:: :.:: .: . .:.:::. ::: KIAA20 EFLNDENKGLDVLVDYLSFAQCSVMFDFEGLESGDDGAF--DKLRSWSRSIEDLQ--PPS 140 150 160 170 180 120 130 140 150 160 FLJ003 --SVP-------KSRHLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFS :.: ..:. ... . .:.::.:::.:::::::::::.::::::::: ::. KIAA20 ALSAPFTNSLARSARQSVLRYSTLPGRRALKNSRLVSQKDDVHVCILCLRAIMNYQYGFN 190 200 210 220 230 240 170 180 190 200 210 220 FLJ003 LVMNHPACVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHR :::.:: :::::::::::::::::::::::::::::::::.::::::::::::: : :: KIAA20 LVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHEIILAAFDNFKEVCKELHR 250 260 270 280 290 300 230 240 250 260 270 280 FLJ003 FEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLERLRLT :::::::::::::::::::::::::::::::::.::::: ::::::.:::. .:.. : : KIAA20 FEKLMEYFRNEDSNIDFMVACMQFINIVVHSVEDMNFRVHLQYEFTKLGLEEFLQKSRHT 310 320 330 340 350 360 290 300 310 320 330 340 FLJ003 ESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALVRVGPDPSPAHHRPT ::.::::::::::::.::::.::::.::::..::..:::.:.:. KIAA20 ESEKLQVQIQAYLDNVFDVGGLLEDAETKNVALEKVEELEEHVSHLTEKLLDLENENMMR 370 380 390 400 410 420 350 360 370 380 FLJ003 GHTGHRATQAWALGSRQCYNCALEVLPAWPQSLA KIAA20 VAELEKQLLQREKELESIKETYENTSHQVHTLRRLIKEKEEAFQRRCHLEPNVRGLESVD 430 440 450 460 470 480 >>KIAA0666 ( 1085 res) hk02075 (1085 aa) initn: 291 init1: 218 opt: 316 Z-score: 284.7 bits: 62.9 E(): 1.2e-10 Smith-Waterman score: 318; 30.000% identity (64.583% similar) in 240 aa overlap (144-363:179-414) 120 130 140 150 160 170 FLJ003 PPSSVPKSRHLTIKLTPAHSRKALRNSRIVSQKDDVHVCIM-CLRAIMNYQSGFSLVMNH .... .:. .. :..:.:: ..: . :. : KIAA06 RTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAH 150 160 170 180 190 200 180 190 200 210 220 230 FLJ003 PACVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHRFEKLM .: :: ::...: .::. :::.:.::::: ::: .: :. .... .:. ::. :. KIAA06 SESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLI 210 220 230 240 250 260 240 250 260 270 280 FLJ003 EYF-----RNEDSNIDFMVACMQFINIVVHS---VENMNFRVFLQYEFTHLGLDLYLERL . . : .: .... .: :.::: :. . ::...::. :.::: ::.. ...: KIAA06 NDLDKSTGRYRD-EVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKL 270 280 290 300 310 320 290 300 310 320 330 FLJ003 RLTESDKLQVQIQAYLDNIFDVGAL-------LEDTETKNAVLEHMEELQEQVALVRVGP : :.. :. ... ... . . : : .::.:. . .: ..... .. : KIAA06 REHENSTLDRHLD-FFEMLRNEDELEFAKRFELVHIDTKSAT-QMFELTRKRLTHSEAYP 330 340 350 360 370 380 340 350 360 370 380 FLJ003 D-PSPAHH---RPTGHTGHRATQAWALGSRQCYNCALEVLPAWPQSLA : :: : ..:. ..: : : .: KIAA06 HFMSILHHCLQMPYKRSGN-TVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVR 390 400 410 420 430 440 >>KIAA0381 ( 1114 res) ah04695 (1114 aa) initn: 300 init1: 213 opt: 308 Z-score: 277.5 bits: 61.6 E(): 2.9e-10 Smith-Waterman score: 322; 30.189% identity (59.245% similar) in 265 aa overlap (62-299:116-377) 40 50 60 70 80 FLJ003 LFHHYVQHSAKNTASSSCSLVSPAGLSLLAFPLPPLK-WPLQC------WPPAPLHPV-P : ::: : : . : : : : KIAA03 SPIPNAEELNIRFAELVDELDLTDKNREAMFALPPEKKWQIYCSKKKEQEDPNKLATSWP 90 100 110 120 130 140 90 100 110 120 130 FLJ003 RYDMESTDNGAS-------NSEKNKPLEQSVEDLSKGPPSSVPKSRHLT--IKLTPAHSR : .. .. :. . :... .: :::: : . : : .: :.: KIAA03 DYYIDRINSMAAMQSLYAFDEEETEMRNQVVEDL-KTALRTQPM-RFVTRFIELEGLTCL 150 160 170 180 190 200 140 150 160 170 180 190 FLJ003 -KALRNSRIVSQKDDVHVCIM-CLRAIMNYQSGFSLVMNHPACVNEIALSLNNKNPRTKA . ::. .. .. .:. .. :..:.:: ..: . :. .: .. :: :: ..: .::. KIAA03 LNFLRSMDHATCESRIHTSLIGCIKALMNNSQGRAHVLAQPEAISTIAQSLRTENSKTKV 210 220 230 240 250 260 200 210 220 230 240 FLJ003 LVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHRFEKLMEYF-----RNEDSNIDFMVA :::.:.::::: ::: .: :. ... .:. ::. :.. . : .: .... .: KIAA03 AVLEILGAVCLVPGGHKKVLQAMLHYQVYAAERTRFQTLLNELDRSLGRYRD-EVNLKTA 270 280 290 300 310 320 250 260 270 280 290 300 FLJ003 CMQFINIVVHS---VENMNFRVFLQYEFTHLGLDLYLERLRLTESDKLQVQIQAYLDNIF :.::: :... .:..::. :.::: ::.. ...:: :. :. ... . KIAA03 IMSFINAVLNAGAGEDNLEFRLHLRYEFLMLGIQPVIDKLRQHENAILDKHLDFFEMVRN 330 340 350 360 370 380 310 320 330 340 350 360 FLJ003 DVGALLEDTETKNAVLEHMEELQEQVALVRVGPDPSPAHHRPTGHTGHRATQAWALGSRQ KIAA03 EDDLELARRFDMVHIDTKSASQMFELIHKKLKYTEAYPCLLSVLHHCLQMPYKRNGGYFQ 390 400 410 420 430 440 >>FLJ00366 ( 282 res) sh05203 (282 aa) initn: 67 init1: 67 opt: 97 Z-score: 97.2 bits: 26.3 E(): 3.2 Smith-Waterman score: 97; 25.166% identity (49.007% similar) in 151 aa overlap (44-187:30-177) 20 30 40 50 60 70 FLJ003 IKALRSGMRAAPDREGWPLFHHYVQHSAKNTASSSCSLVSPAGLSLLAFPLPPLKWPLQC :: :. : : : .: ::: ::. FLJ003 PLSPDHRTAAASTSAVVLSSASPGWSLLPTAMSGECLHSTCPAPRLFLP-PPLAVPLSR 10 20 30 40 50 80 90 100 110 120 FLJ003 WPPA------PLHPVPRYDMESTDNGASNSEKNKPLEQSVEDLSKGPPSSVPKSRHLTIK ::: : : .. . . . .::.: . : : . :.. .. .: FLJ003 WPPCSCLIPPPCSPGRHFVVLGEYDRSSNAEPLQVLSVSRAITHPSWNSTTMNNDVTLLK 60 70 80 90 100 110 130 140 150 160 170 180 FLJ003 L-TPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIALSLNNK : .::. . ..:... . . :. . . :: . : :: ....:: : . FLJ003 LASPAQYTTRISPVCLASSNEALTEGLTCVTTGWGRLSGVGNVT--PAHLQQVALPLVTV 120 130 140 150 160 170 190 200 210 220 230 240 FLJ003 NPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEQHRFEKLMEYFRNEDSNIDFMV : FLJ003 NQCRQYWGSSITDSMICAGGAGASSCQVSPSTRSSALSCGIPPQPPLLNSPSLFPLRVTP 180 190 200 210 220 230 >>FLJ00232 ( 318 res) sj06640 (318 aa) initn: 59 init1: 59 opt: 95 Z-score: 94.9 bits: 26.0 E(): 4.4 Smith-Waterman score: 95; 28.226% identity (50.806% similar) in 124 aa overlap (266-381:149-266) 240 250 260 270 280 290 FLJ003 RNEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEFTHLGLDLYLERLRLTESDKLQVQ :.::. :.:. .:: : ..: . : : FLJ002 FLFISRSKPLKTLSRERPLPNIFNLYTILTVMLQF-FVHFLSLVYLYREAQARSPEKQEQ 120 130 140 150 160 170 300 310 320 330 340 FLJ003 -IQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALVRVGPDPS-------PAHHRPT .. : . :. .:...: :. .: . . :: :: :. : .:. FLJ002 FVDLYKE--FE-PSLVNSTVYIMAMAMQMATFAINYK-VRPGPCPNIHCLPTQPHPMKPS 180 190 200 210 220 230 350 360 370 380 FLJ003 GHTGHRATQAWALGSRQCYNCALEVLPAWPQSLA ::: .: : :.. . .:: ::: FLJ002 VPHPHRARPSWRACPRTSPWCGVWQFHSWP-SLACSSAPRPTSTASLASWTSLWSSSWSL 240 250 260 270 280 290 FLJ002 PRSCSWTSAWRSWPTASCSSSWGPRS 300 310 >>KIAA2011 ( 1091 res) pj02017 (1091 aa) initn: 83 init1: 49 opt: 100 Z-score: 93.4 bits: 27.5 E(): 5.3 Smith-Waterman score: 100; 29.167% identity (53.333% similar) in 120 aa overlap (39-147:480-597) 10 20 30 40 50 60 FLJ003 CRETGIKALRSGMRAAPDREGWPLFHHYVQHSAKNTASSSCS----LVSPAGLS--LLAF : ::.:. .: . :.::. . ...: KIAA20 KSPVPFLPGTSPSADGPDSFSCVFEAILESHRAKGTSYTSLASLEALASPGPTQSPFFTF 450 460 470 480 490 500 70 80 90 100 110 FLJ003 PLPPLK-WPLQCWP-PAPLHPV-PRYDMESTDNGASNSEKNKPLEQSVEDLSKG--PPSS ::: : : :::: :. : :. .: ... .. .. :. : ::: KIAA20 ELPPQPPAPRPDPPAPAPLAPLEPDSGTSSAADGPWTQRGEEEEAEARAKLAPGREPPSP 510 520 530 540 550 560 120 130 140 150 160 170 FLJ003 VPKSRHLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVN . : . .: :. : :..::..: KIAA20 CHSEDSLGLGAAPLGSEPPL--SQLVSDSDSELDSTERLALGSTDTLSNGQKADLEAAQR 570 580 590 600 610 620 >>KIAA1629 ( 1329 res) fh20517 (1329 aa) initn: 76 init1: 46 opt: 100 Z-score: 92.5 bits: 27.6 E(): 6 Smith-Waterman score: 100; 27.826% identity (54.783% similar) in 115 aa overlap (40-147:306-413) 10 20 30 40 50 60 FLJ003 RETGIKALRSGMRAAPDREGWPLFHHYVQHSAKNTASSSCSLVSPAGLSLLAFPLPPLKW :::..::.::. :.. : . ::: KIAA16 KNDTSLPSVAPSKTKSSSKLSSCIAAIAALSAKKAASDSCK--EPVANSRESSPLPK--- 280 290 300 310 320 330 70 80 90 100 110 120 FLJ003 PLQCWPPAPLHPVPRYDMESTDNGASNSEKNKPLEQSVEDLSKGPPSS-------VPKSR .. : : . .. . . : .. ..: : :. ::: ::: .:: : KIAA16 EVNDSPRAADKSPESQNLIDGTKKPSLKQPDSPRSISSENSSKGSPSSPAGSTPAIPKVR 340 350 360 370 380 390 130 140 150 160 170 180 FLJ003 HLTIKLTPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIALS ::: . .. .... .:.. . : KIAA16 IKTIKTSSGEIKRTV--TRVLPEVDLDSGKKPSEQTASVMASVTSLLSSPASAAVLSSPP 400 410 420 430 440 >>FLJ00079 ( 84 res) as00079 (84 aa) initn: 82 init1: 54 opt: 84 Z-score: 91.6 bits: 23.5 E(): 6.7 Smith-Waterman score: 84; 43.182% identity (59.091% similar) in 44 aa overlap (53-96:32-69) 30 40 50 60 70 80 FLJ003 AAPDREGWPLFHHYVQHSAKNTASSSCSLVSPAGLSLLAFPLPPLKWPLQCWPPAPLHPV : ::.. :::: : : ::. : : FLJ000 VPLCFFPLLQMAVGPPDCPVGGPLTFPGRGSGAGVGTTLTPLPPPKMP----PPTILSTV 10 20 30 40 50 90 100 110 120 130 140 FLJ003 PRYDMESTDNGASNSEKNKPLEQSVEDLSKGPPSSVPKSRHLTIKLTPAHSRKALRNSRI :: .: : : :... FLJ000 PR-QMFS-DAGSGDDALDGDDDLVIDIPE 60 70 80 >>FLJ00261 ( 1651 res) sh06537 (1651 aa) initn: 72 init1: 72 opt: 98 Z-score: 89.6 bits: 27.4 E(): 8.5 Smith-Waterman score: 98; 31.944% identity (51.389% similar) in 72 aa overlap (52-121:881-950) 30 40 50 60 70 FLJ003 RAAPDREGWPLFHHYVQHSAKNTASSSCSLVSPAGLSLLAFPLPPLKWPLQCWP--PAPL .: : : . ::::: :. : : :: FLJ002 SSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLPPL--PIIDPPTEPPPL 860 870 880 890 900 80 90 100 110 120 130 FLJ003 HPVPRYDMESTDNGASNSEKNKPLEQSVEDLSKGPPSSVPKSRHLTIKLTPAHSRKALRN : : .:: .: :: . . . .: . :.:. .. FLJ002 PPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFTSTSR 910 920 930 940 950 960 140 150 160 170 180 190 FLJ003 SRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIALSLNNKNPRTKALVLELLA FLJ002 SKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQ 970 980 990 1000 1010 1020 381 residues in 1 query sequences 2210613 residues in 2457 library sequences Tcomplib [34.26] (2 proc) start: Fri Feb 27 10:51:22 2009 done: Fri Feb 27 10:51:22 2009 Total Scan time: 0.500 Total Display time: 0.080 Function used was FASTA [version 34.26.5 April 26, 2007]