# /usr/local/bin/fasta34_t -T 4 -b50 -d10 -H -O./tmp/sh07670.fasta.huge -Q ../query/FLJ00321.ptfa ./tmplib.10216 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00321, 245 aa vs ./tmplib.10216 library 2210749 residues in 2457 sequences Expectation_n fit: rho(ln(x))= 3.9564+/-0.00445; mu= 20.6550+/- 0.303 mean_var=103.3849+/-21.541, 0's: 0 Z-trim: 3 B-trim: 0 in 0/40 Lambda= 0.126138 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, open/ext: -10/-2, width: 16 The best scores are: opt bits E(2457) FLJ00053 ( 443 res) as00053 ( 443) 737 143.5 1.7e-35 FLJ00283 ( 500 res) sh02088 ( 500) 575 114.1 1.3e-26 FLJ00139 ( 585 res) sh03241 ( 585) 106 28.9 0.7 FLJ00232 ( 318 res) sj06640 ( 318) 92 25.9 3 FLJ00193 ( 491 res) sj02629 ( 491) 92 26.2 3.8 KIAA1150 ( 499 res) hg02066 ( 499) 90 25.9 4.9 FLJ00403 ( 225 res) sj07657 ( 225) 84 24.2 7 KIAA0749 ( 678 res) hk04328 ( 678) 87 25.6 8.3 KIAA0420 ( 756 res) fg02987 ( 756) 87 25.6 8.7 KIAA1862 ( 1070 res) aj00563 (1070) 88 26.1 9.2 FLJ00134 ( 341 res) sh02437 ( 341) 82 24.1 11 KIAA0557 ( 493 res) hh01334 ( 493) 83 24.6 12 KIAA1113 ( 1131 res) hj06379(revised) (1131) 86 25.7 12 KIAA0282 ( 711 res) ha06845s1 ( 711) 84 25.0 12 KIAA0929 ( 1663 res) hh03374 (1663) 87 26.2 13 KIAA0706 ( 1123 res) pj01355 (1123) 85 25.6 14 KIAA0403 ( 416 res) hg01159 ( 416) 81 24.1 14 KIAA1908 ( 445 res) ah02604 ( 445) 81 24.2 14 FLJ00104 ( 287 res) as00104 ( 287) 79 23.5 15 KIAA0200 ( 1042 res) ha02508 (1042) 83 25.1 17 KIAA0508 ( 253 res) hh00207 ( 253) 77 23.0 18 FLJ00236 ( 702 res) sj07289 ( 702) 81 24.5 18 KIAA0485 ( 83 res) hg00837 ( 83) 72 21.3 19 KIAA0963 ( 1391 res) hj05936 (1391) 83 25.3 20 KIAA1865 ( 723 res) fj14303 ( 723) 80 24.3 21 FLJ00016 ( 349 res) as00016 ( 349) 77 23.3 21 KIAA1987 ( 357 res) fk04103 ( 357) 77 23.3 21 FLJ00360 ( 137 res) sh04326 ( 137) 73 21.9 22 FLJ00163 ( 521 res) sh06933 ( 521) 78 23.7 23 KIAA1133 ( 891 res) hg03758b ( 891) 80 24.5 23 KIAA0047 ( 268 res) ha01551 ( 268) 74 22.5 27 KIAA0654 ( 1267 res) fh18335 (1267) 80 24.7 27 KIAA0479 ( 340 res) hh00797 ( 340) 74 22.7 30 KIAA2020 ( 572 res) ph01249 ( 572) 76 23.4 30 KIAA0992 ( 772 res) hk07554 ( 772) 77 23.8 31 KIAA1120 ( 1024 res) hg02941 (1024) 78 24.2 31 KIAA1481 ( 1380 res) fj05724 (1380) 79 24.6 32 KIAA0309 ( 3053 res) hg00096s1 (3053) 82 25.7 33 KIAA0620 ( 1985 res) hg04174 (1985) 80 25.1 34 KIAA1675 ( 1286 res) ae00102 (1286) 78 24.4 35 KIAA0339 ( 1709 res) hg01304(revised) (1709) 79 24.8 36 KIAA1096 ( 1919 res) pf00402 (1919) 79 24.9 38 KIAA0685 ( 939 res) hk02959 ( 939) 76 23.8 39 KIAA0495 ( 209 res) hg00106 ( 209) 70 21.6 39 KIAA1741 ( 1777 res) fh23254 (1777) 78 24.6 41 FLJ00086 ( 399 res) as00086 ( 399) 72 22.4 42 KIAA1856 ( 1134 res) fh03203 (1134) 76 23.9 43 KIAA1079 ( 1476 res) hj06972 (1476) 77 24.3 43 KIAA1206 ( 1912 res) fg04331 (1912) 78 24.7 43 KIAA0729 ( 1196 res) hk03615 (1196) 76 24.0 44 >>FLJ00053 ( 443 res) as00053 (443 aa) initn: 738 init1: 705 opt: 737 Z-score: 730.8 bits: 143.5 E(): 1.7e-35 Smith-Waterman score: 737; 61.290% identity (73.272% similar) in 217 aa overlap (37-243:245-443) 10 20 30 40 50 FLJ003 PDPQVCCLERAGRAEPGSLRLGEGRWMRVAGSPPP----PHFN---HTQASLQEQRGPGT : : : :.. .. :.:....: :: FLJ000 RPISLVRPVLPATLSHGIPWPVACRECFSGGVPTPWLICPRLAGDIRSTANLSRSQGRGT 220 230 240 250 260 270 60 70 80 90 100 110 FLJ003 WPSPLVSCCSAPRSWPVLWVVTVGWLVTVGRLKCSLRELFLA-WPPGLLDGTADDATTVD .. :.:.. . : .. : . : : :. : :::::::::::::::: FLJ000 H----LAFVSSPQA-----LRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATTVD 280 290 300 310 320 120 130 140 150 160 170 FLJ003 TLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: FLJ000 TLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVG 330 340 350 360 370 380 180 190 200 210 220 230 FLJ003 GHLCGTLAGCVRVQRAALDFLGTLSQGTG--ECVLYGFGVGLSLCPHSPFCSEAPGNCCP ::::::::::::::::::::::::::::: : : ...: : : :.::. : FLJ000 GHLCGTLAGCVRVQRAALDFLGTLSQGTGPQELVTQALAVLLE-------CLESPGSS-P 390 400 410 420 430 240 FLJ003 SLFLLWGGG .. :: FLJ000 TVRW-WG 440 >>FLJ00283 ( 500 res) sh02088 (500 aa) initn: 631 init1: 560 opt: 575 Z-score: 571.0 bits: 114.1 E(): 1.3e-26 Smith-Waterman score: 575; 62.893% identity (75.472% similar) in 159 aa overlap (37-187:245-394) 10 20 30 40 50 FLJ003 PDPQVCCLERAGRAEPGSLRLGEGRWMRVAGSPPP----PHFN---HTQASLQEQRGPGT : : : :.. .. :.:....: :: FLJ002 RPISLVRPVLPATLSHGVPWPVACRECFSGGVPTPWLICPRLAGDIRSTANLSRSQGRGT 220 230 240 250 260 270 60 70 80 90 100 110 FLJ003 WPSPLVSCCSAPRSWPVLWVVTVGWLVTVGRLKCSLRELFLA-WPPGLLDGTADDATTVD .. :.:.. . : .. : . : : :. : :::::::::::::::: FLJ002 ----HLAFVSSPQA-----LRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATTVD 280 290 300 310 320 120 130 140 150 160 170 FLJ003 TLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: FLJ002 TLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVG 330 340 350 360 370 380 180 190 200 210 220 230 FLJ003 GHLCGTLAGCVRVQRAALDFLGTLSQGTGECVLYGFGVGLSLCPHSPFCSEAPGNCCPSL :::::: .: FLJ002 GHLCGTPGGLRPGPASSPRLPGDAVTGDRPPGAGDAGACCPPGVPREPRLQPHGTVVGIE 390 400 410 420 430 440 >>FLJ00139 ( 585 res) sh03241 (585 aa) initn: 77 init1: 48 opt: 106 Z-score: 109.1 bits: 28.9 E(): 0.7 Smith-Waterman score: 110; 24.051% identity (46.835% similar) in 158 aa overlap (5-150:334-489) 10 20 30 FLJ003 RPRFRPPDPQVCCLERAGRAEPGSLRLGEGRWMR :: . .. :...: . : FLJ001 AGNSPARASVPAAPNPAATSATSVHVRSPARPSESRLAPTPTEGKVRPRVTNSSPMGWSS 310 320 330 340 350 360 40 50 60 70 80 FLJ003 VA------GSPP--PPHFNHTQASLQEQRGPGTWPSPLVSCCSAPRSWPVLWVVTVGWLV .: .: : :: . . . : .: .. :. : . : .: FLJ001 AAPCTAAAASHPAVPPSAPDPRPATPQGGGAPRVAAPQTTLSSSSTSAAT--VDPPAWTP 370 380 390 400 410 420 90 100 110 120 130 140 FLJ003 TVGRLKCSLRELFL--AWPPGL-LDGTADDATTVDTLLASKSSCAGLLCRTLAHLEEL-Q ...: . . ..: : ::: :.: . . : . .:: . ..: ..:. ::: FLJ001 SASRTQQARNKFFQTSAVPPGTSLSGRGPTPSLVLSKDSSKEQARNFLKQALSALEEAGA 430 440 450 460 470 480 150 160 170 180 190 200 FLJ003 PLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGHLCGTLAGCVRVQRAALDFLGTL : : :::: FLJ001 PAPGRPSPATAAVPSSQPKTEAPQASPLAKPLQSSSPRVLGLPSRMEPPAPLSTSSTSQA 490 500 510 520 530 540 >>FLJ00232 ( 318 res) sj06640 (318 aa) initn: 93 init1: 48 opt: 92 Z-score: 97.7 bits: 25.9 E(): 3 Smith-Waterman score: 98; 26.087% identity (44.348% similar) in 115 aa overlap (3-103:211-306) 10 20 30 FLJ003 RPRFRP-PDPQVCCLERAGRAEPGSLRLGEGR . :: : :.. :: ..: .. FLJ002 LYKEFEPSLVNSTVYIMAMAMQMATFAINYKVRPGPCPNIHCLP----TQPHPMK----- 190 200 210 220 230 40 50 60 70 FLJ003 WMRVAGSPPPPHFNHTQASLQEQRGP--GTWPS---PLVSCCSAPR--------SWPVLW : : :: . . . .: :.: : ..: :::: :: :: FLJ002 -----PSVPHPHRARPSWRACPRTSPWCGVWQFHSWPSLACSSAPRPTSTASLASWTSLW 240 250 260 270 280 80 90 100 110 120 130 FLJ003 VVTVGWLVTVGRLKCSLRELFLAWPPGLLDGTADDATTVDTLLASKSSCAGLLCRTLAHL . .: .. : .:: . .:: FLJ002 --SSSW--SLPR-SCSWTSAWRSWPTASCSSSWGPRS 290 300 310 >>FLJ00193 ( 491 res) sj02629 (491 aa) initn: 93 init1: 45 opt: 92 Z-score: 96.1 bits: 26.2 E(): 3.8 Smith-Waterman score: 92; 37.879% identity (56.061% similar) in 66 aa overlap (22-77:389-452) 10 20 30 40 FLJ003 RPRFRPPDPQVCCLERAGRAEPGSLRLGEGRWMRV----AGSPP--PPHFN ::. : :. .:.. .:::: ::.: FLJ001 GLISEEELGASVASLSSENPYATIRDLPSLPGGPR--ESSYMEMKGPPSGSPPRQPPQFW 360 370 380 390 400 410 50 60 70 80 90 100 FLJ003 HTQASLQEQ--RGPGTW--PSPLVSCCSAPRSWPVLWVVTVGWLVTVGRLKCSLRELFLA .: : : : ::. ::::. .. : : : FLJ001 DSQRRRQPQPQRDSGTYEQPSPLIHDRDSVGSQPPLPPGLPPGHYDSPKNSHIPGHYDLP 420 430 440 450 460 470 110 120 130 140 150 160 FLJ003 WPPGLLDGTADDATTVDTLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATV FLJ001 PVRHPPSPPLRRQDR 480 490 >>KIAA1150 ( 499 res) hg02066 (499 aa) initn: 68 init1: 46 opt: 90 Z-score: 94.0 bits: 25.9 E(): 4.9 Smith-Waterman score: 90; 26.351% identity (47.973% similar) in 148 aa overlap (21-157:141-283) 10 20 30 40 FLJ003 RPRFRPPDPQVCCLERAGRAEPGSLRLGEGR-WMRVAGSPPPPHFNHTQ- :: .:: .:. .: : . ::. .: KIAA11 NAASIVQPSPAHVGQQGLSKLPSRPGAQGVEPQNLRTLQGHSVIRSATNTTLPHMLMSQR 120 130 140 150 160 170 50 60 70 80 90 100 FLJ003 ------ASLQEQRGPGTWPSPLVSCCSAPRSWPVL-WVVTVGWLVTVGRLKCSLRELFLA :.:: :::: :.: . ..: :.. . . : : : . :: KIAA11 VIAPNPAQLQGQRGP---PKPGLVRTTTPNMNPAINYQPQSSSSVPCQRTTSSAIYMNLA 180 190 200 210 220 110 120 130 140 150 FLJ003 --WPPGLLDGTADDATTVDTLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGA :: .. ... . ... . .: :. .:: . : . : : : : :: KIAA11 SHIQPGTVNRVSSPLPSPSAMTDAANSQAAAKLALRKQLE--KTLLEIPPPKPPAPLLHF 230 240 250 260 270 280 160 170 180 190 200 210 FLJ003 TVTVLRLCDGSAAPASSVGGHLCGTLAGCVRVQRAALDFLGTLSQGTGECVLYGFGVGLS KIAA11 LPSAANSEFIYMVGLEEVVQSVIDSQGKSCASLLRVEPFVCAQCRTDFTPHWKQEKNGKI 290 300 310 320 330 340 >>FLJ00403 ( 225 res) sj07657 (225 aa) initn: 57 init1: 57 opt: 84 Z-score: 91.2 bits: 24.2 E(): 7 Smith-Waterman score: 84; 40.476% identity (64.286% similar) in 42 aa overlap (207-245:167-203) 180 190 200 210 220 230 FLJ003 VGGHLCGTLAGCVRVQRAALDFLGTLSQGTGECVLYGFGV-GLSLCPHSPFCSEAPGNCC :.: : :. ::.: :..:.:: : . FLJ004 LFPDDVATLSPGSWLPCPRCEHPTSTERWGGQC---GAGTRGLDLYPQAPICSFADPD-- 140 150 160 170 180 190 240 FLJ003 PSLFLLWG--GG : . ..:: :: FLJ004 PFVCVIWGHFGGIFGRCGPGWVCARDAGCWCHKR 200 210 220 >>KIAA0749 ( 678 res) hk04328 (678 aa) initn: 96 init1: 71 opt: 87 Z-score: 89.9 bits: 25.6 E(): 8.3 Smith-Waterman score: 90; 30.702% identity (44.737% similar) in 114 aa overlap (6-110:167-264) 10 20 30 FLJ003 RPRFRPPDPQVCCLERAGRAEPGSLRLGEGRWMRV ::. .. : : : : . : :: : KIAA07 PVGRAPRPSAQPQSDPGSAWAGPWGGRRPGPPSYEAHLLLR-GSAGTAPRR----RWDRP 140 150 160 170 180 190 40 50 60 70 80 90 FLJ003 AGSPPPPHFNHTQASLQEQRGPGTWPSPLVSCCSAPRSWPVLWVVTVGWLVTVGRLKCSL :: .. . .: .::::. : ::: . :. : : :: : : KIAA07 PPYVAPPSYEGPHRTLGTKRGPGNSQVP---TSSAPAATPA---RTDG-----GRTKKRL 200 210 220 230 240 100 110 120 130 140 FLJ003 -----RELFLAWP----PGLLDGTADDATTVDTLLASKSSCAGLLCRTLAHLEELQPLPQ :... :: ::: :. KIAA07 DPRIYRDVLGAWGLRQGQGLLGGSPGCGAARARPEPGKGVVEKSLGLAAADLNSGSDSHP 250 260 270 280 290 300 >>KIAA0420 ( 756 res) fg02987 (756 aa) initn: 87 init1: 57 opt: 87 Z-score: 89.5 bits: 25.6 E(): 8.7 Smith-Waterman score: 87; 28.713% identity (50.495% similar) in 101 aa overlap (70-165:484-579) 40 50 60 70 80 90 FLJ003 PPPHFNHTQASLQEQRGPGTWPSPLVSCCSAPRSWPVLWVVTVGWLVTVGRLKCSLRELF ::: .::::.. .. : : . KIAA04 WRPGVKALLRMIEVVEDNYPETLGRLLIVRAPRVFPVLWTLISPFINENTRRKFLIYSGS 460 470 480 490 500 510 100 110 120 130 140 150 FLJ003 -LAWPPGLLDGTADDATTVDTLLASKSSC----AGLLCRTLAHLEELQPLPQRPSPWPQA : ::.: : .. .:...: : .::. ..: :: : . . : : KIAA04 NYQGPGGLVDYL--DREVIPDFLGGESVCNVPEGGLVPKSLYMTEEEQ--EHTDQLW-QW 520 530 540 550 560 160 170 180 190 200 210 FLJ003 SLLGATVTVLRLCDGSAAPASSVGGHLCGTLAGCVRVQRAALDFLGTLSQGTGECVLYGF : ...::: KIAA04 SETYHSASVLRGAPHEVAVEILEGESVITWDFDILRGDVVFSLYHTKQAPRLGAREPGTR 570 580 590 600 610 620 >>KIAA1862 ( 1070 res) aj00563 (1070 aa) initn: 109 init1: 52 opt: 88 Z-score: 89.1 bits: 26.1 E(): 9.2 Smith-Waterman score: 96; 25.455% identity (41.212% similar) in 165 aa overlap (9-148:611-771) 10 20 30 FLJ003 RPRFRPPDPQV----C---CLERAGRAEPGSLRLGEGR ::: : : .: : .::. : : : KIAA18 KEGLRAEACESARLGQGRGEAPTRSLHLVSPQVFTSSCVPACHQR-GFKDPGATRPGVWR 590 600 610 620 630 40 50 60 70 FLJ003 WMRVAGSPPPPHFNHTQASLQ------EQR-----GPGTWPSPLVSCCSA------PRSW :. ...: : .. ...:. : ::. ::: : . :: KIAA18 WLPEGSAPKPSPLHCLESALRGILPVRPLRFACVGGPSPSPSPGSSSSFSGSEGEDPRPE 640 650 660 670 680 690 80 90 100 110 120 130 FLJ003 PVLWVVTVGWLVTVGRL-KCSLRELFLAWPPGLLDGTADDATTVDTLLASKSSCAGLLCR : :: :: .:. . : : :.. . : . :.:: KIAA18 PELWKPLPQ---ERDRLPSCKPPVPLSPCPGGTPAGSSGGSPGEDPRRTEPRYCSGLGAG 700 710 720 730 740 750 140 150 160 170 180 190 FLJ003 TLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGHLCGTLAGCVRVQR : .. : .:: KIAA18 TAQDPCPVSQLEKRPRVSEASRGLELGHGRPRVAAKTHERLLPQGPPELPSESPPPELPP 760 770 780 790 800 810 245 residues in 1 query sequences 2210749 residues in 2457 library sequences Tcomplib [34.26] (2 proc) start: Fri Feb 27 10:51:44 2009 done: Fri Feb 27 10:51:45 2009 Total Scan time: 0.410 Total Display time: 0.030 Function used was FASTA [version 34.26.5 April 26, 2007]