# /usr/local/bin/fasta34_t -T 8 -b50 -d10 -E0.01 -H -O./tmp/sj01842.fasta.nr -Q ../query/FLJ00265.ptfa /cdna2/lib/nr/nr 2 FASTA searches a protein or DNA sequence data bank version 34.26.5 April 26, 2007 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 FLJ00265, 155 aa vs /cdna2/lib/nr/nr library 2693465022 residues in 7827732 sequences statistics sampled from 60000 to 7802279 sequences Expectation_n fit: rho(ln(x))= 4.9733+/-0.000183; mu= 7.3424+/- 0.010 mean_var=69.6604+/-13.670, 0's: 30 Z-trim: 168 B-trim: 362 in 1/64 Lambda= 0.153667 FASTA (3.5 Sept 2006) function [optimized, BL50 matrix (15:-5)] ktup: 2 join: 36, opt: 24, open/ext: -10/-2, width: 16 The best scores are: opt bits E(7827732) gi|28273118|dbj|BAC56922.1| FLJ00265 protein [Homo ( 155) 1048 240.5 7.4e-62 gi|118573873|sp|Q86YV6.2|MYLK4_HUMAN RecName: Full ( 388) 912 210.7 1.8e-52 gi|124376290|gb|AAI32834.1| Myosin light chain kin ( 388) 912 210.7 1.8e-52 gi|114605202|ref|XP_527218.2| PREDICTED: hypotheti ( 388) 909 210.0 2.8e-52 gi|109069431|ref|XP_001090801.1| PREDICTED: simila ( 385) 889 205.6 6e-51 gi|149731757|ref|XP_001488116.1| PREDICTED: myosin ( 389) 855 198.1 1.1e-48 gi|74003969|ref|XP_545312.2| PREDICTED: similar to ( 405) 807 187.4 1.9e-45 gi|118573874|sp|Q5SUV5.2|MYLK4_MOUSE RecName: Full ( 386) 803 186.5 3.3e-45 gi|126322069|ref|XP_001368387.1| PREDICTED: hypoth ( 384) 797 185.2 8.3e-45 gi|109505717|ref|XP_001066672.1| PREDICTED: simila ( 578) 787 183.1 5.3e-44 gi|149045261|gb|EDL98347.1| rCG44136, isoform CRA_ ( 680) 787 183.2 6e-44 gi|148700415|gb|EDL32362.1| mCG17885 [Mus musculus ( 781) 787 183.2 6.6e-44 gi|194037955|ref|XP_001924656.1| PREDICTED: simila ( 544) 778 181.1 2e-43 gi|194037949|ref|XP_001924502.1| PREDICTED: simila ( 641) 778 181.2 2.3e-43 gi|149412799|ref|XP_001510079.1| PREDICTED: hypoth ( 521) 774 180.2 3.6e-43 gi|118086495|ref|XP_418978.2| PREDICTED: hypotheti ( 375) 718 167.7 1.5e-39 gi|189533127|ref|XP_697394.2| PREDICTED: hypotheti ( 668) 690 161.7 1.8e-37 gi|189515517|ref|XP_695224.3| PREDICTED: similar t ( 610) 644 151.4 1.9e-34 gi|94734142|emb|CAK04605.1| novel kinase domain co ( 640) 614 144.8 2e-32 gi|194677911|ref|XP_596207.4| PREDICTED: similar t ( 313) 608 143.2 2.9e-32 gi|118096343|ref|XP_414113.2| PREDICTED: similar t ( 921) 594 140.5 5.7e-31 gi|149579802|ref|XP_001521045.1| PREDICTED: simila ( 729) 592 140.0 6.5e-31 gi|74197329|dbj|BAE43277.1| unnamed protein produc ( 191) 583 137.5 9.3e-31 gi|94384240|ref|XP_992905.1| PREDICTED: hypothetic ( 506) 587 138.7 1.1e-30 gi|126296194|ref|XP_001369857.1| PREDICTED: hypoth ( 983) 587 139.0 1.8e-30 gi|148679062|gb|EDL11009.1| mCG14341 [Mus musculus ( 248) 580 136.9 1.8e-30 gi|221042714|dbj|BAH13034.1| unnamed protein produ ( 478) 582 137.6 2.2e-30 gi|26343041|dbj|BAC35177.1| unnamed protein produc ( 724) 583 138.0 2.6e-30 gi|123791378|sp|Q3UIZ8.1|MYLK3_MOUSE RecName: Full ( 795) 583 138.0 2.8e-30 gi|158937333|sp|Q32MK0.2|MYLK3_HUMAN RecName: Full ( 795) 582 137.8 3.2e-30 gi|119603093|gb|EAW82687.1| MLCK protein [Homo sap ( 795) 582 137.8 3.2e-30 gi|14571717|emb|CAC42766.1| myosin light chain kin ( 795) 582 137.8 3.2e-30 gi|197692611|dbj|BAG70269.1| MLCK protein [Homo sa ( 819) 582 137.8 3.3e-30 gi|146219832|ref|NP_872299.2| myosin light chain k ( 819) 582 137.8 3.3e-30 gi|109128385|ref|XP_001113601.1| PREDICTED: simila ( 819) 582 137.8 3.3e-30 gi|194674968|ref|XP_604749.4| PREDICTED: similar t ( 782) 581 137.6 3.7e-30 gi|73977474|ref|XP_532569.2| PREDICTED: similar to (1037) 581 137.6 4.6e-30 gi|159171579|gb|ABW96144.1| cardiac myosin light c ( 786) 579 137.1 5.1e-30 gi|187951825|gb|AAI37984.1| LOC633594 protein [Mus ( 787) 579 137.1 5.1e-30 gi|37606089|emb|CAE49228.1| novel protein [Danio r ( 690) 578 136.8 5.4e-30 gi|157678778|dbj|BAF80631.1| cardiac myosin light ( 715) 578 136.9 5.5e-30 gi|149699469|ref|XP_001490301.1| PREDICTED: myosin ( 809) 577 136.7 7e-30 gi|158937332|sp|Q5RDG7.2|MYLK3_PONAB RecName: Full ( 795) 576 136.4 8.1e-30 gi|55726861|emb|CAH90190.1| hypothetical protein [ ( 819) 576 136.5 8.3e-30 gi|157678782|dbj|BAF80633.1| myosin light chain ki ( 555) 554 131.4 1.8e-28 gi|403440|gb|AAA73168.1| [Gallus domesticus skelet ( 825) 549 130.5 5.3e-28 gi|126293901|ref|XP_001364384.1| PREDICTED: simila ( 864) 527 125.6 1.6e-26 gi|18044433|gb|AAH19408.1| Mylk2 protein [Mus musc ( 240) 517 123.0 2.8e-26 gi|194224275|ref|XP_001499833.2| PREDICTED: myosin ( 597) 521 124.2 3.1e-26 gi|194224277|ref|XP_001499845.2| PREDICTED: myosin ( 624) 521 124.2 3.2e-26 >>gi|28273118|dbj|BAC56922.1| FLJ00265 protein [Homo sap (155 aa) initn: 1048 init1: 1048 opt: 1048 Z-score: 1266.8 bits: 240.5 E(): 7.4e-62 Smith-Waterman score: 1048; 100.000% identity (100.000% similar) in 155 aa overlap (1-155:1-155) 10 20 30 40 50 60 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSFPTDMWSVGVIA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|282 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSFPTDMWSVGVIA 10 20 30 40 50 60 70 80 90 100 110 120 FLJ002 YMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISAS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|282 YMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRISAS 70 80 90 100 110 120 130 140 150 FLJ002 EALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::::::::::::::: gi|282 EALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK 130 140 150 >>gi|118573873|sp|Q86YV6.2|MYLK4_HUMAN RecName: Full=Myo (388 aa) initn: 912 init1: 912 opt: 912 Z-score: 1098.4 bits: 210.7 E(): 1.8e-52 Smith-Waterman score: 912; 100.000% identity (100.000% similar) in 136 aa overlap (20-155:253-388) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|118 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|118 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK :::::::::::::::::::::::::::::::::::::::::::::: gi|118 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK 350 360 370 380 >>gi|124376290|gb|AAI32834.1| Myosin light chain kinase (388 aa) initn: 912 init1: 912 opt: 912 Z-score: 1098.4 bits: 210.7 E(): 1.8e-52 Smith-Waterman score: 912; 100.000% identity (100.000% similar) in 136 aa overlap (20-155:253-388) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|124 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|124 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK :::::::::::::::::::::::::::::::::::::::::::::: gi|124 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK 350 360 370 380 >>gi|114605202|ref|XP_527218.2| PREDICTED: hypothetical (388 aa) initn: 909 init1: 909 opt: 909 Z-score: 1094.8 bits: 210.0 E(): 2.8e-52 Smith-Waterman score: 909; 99.265% identity (100.000% similar) in 136 aa overlap (20-155:253-388) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|114 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|114 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK .::::::::::::::::::::::::::::::::::::::::::::: gi|114 MKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK 350 360 370 380 >>gi|109069431|ref|XP_001090801.1| PREDICTED: similar to (385 aa) initn: 889 init1: 889 opt: 889 Z-score: 1070.9 bits: 205.6 E(): 6e-51 Smith-Waterman score: 889; 99.248% identity (100.000% similar) in 133 aa overlap (20-152:253-385) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|109 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: gi|109 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::::::::::::::::::::::. gi|109 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDL 350 360 370 380 >>gi|149731757|ref|XP_001488116.1| PREDICTED: myosin lig (389 aa) initn: 855 init1: 855 opt: 855 Z-score: 1030.1 bits: 198.1 E(): 1.1e-48 Smith-Waterman score: 855; 94.118% identity (97.794% similar) in 136 aa overlap (20-155:254-389) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|149 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::::::::::::::::::::::::::::::::::::::::::::..::::::: gi|149 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEGREFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::::::::..::::: ::.:: ::: gi|149 IKEKSWRISASEALKHPWLSDHKLHSRLSTQKKKNCCSDTQDRVTK 350 360 370 380 >>gi|74003969|ref|XP_545312.2| PREDICTED: similar to myo (405 aa) initn: 800 init1: 800 opt: 807 Z-score: 972.3 bits: 187.4 E(): 1.9e-45 Smith-Waterman score: 807; 92.308% identity (96.154% similar) in 130 aa overlap (20-149:254-383) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|740 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL ::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::: gi|740 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAREFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::::::::..: :.:. gi|740 IKEKSWRISASEALKHPWLSDHKLHSRLTTQPICPFGTDSFPKLQIRTPPVYLTSPFGPL 350 360 370 380 390 400 gi|740 QS >>gi|118573874|sp|Q5SUV5.2|MYLK4_MOUSE RecName: Full=Myo (386 aa) initn: 825 init1: 803 opt: 803 Z-score: 967.8 bits: 186.5 E(): 3.3e-45 Smith-Waterman score: 803; 94.488% identity (97.638% similar) in 127 aa overlap (20-146:253-379) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|118 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::.:::::::::::::::::::.::::::::::::::::::::::::::::: gi|118 STDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::::::::.:::. : : gi|118 IKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK 350 360 370 380 >>gi|126322069|ref|XP_001368387.1| PREDICTED: hypothetic (384 aa) initn: 797 init1: 797 opt: 797 Z-score: 960.7 bits: 185.2 E(): 8.3e-45 Smith-Waterman score: 797; 90.840% identity (95.420% similar) in 131 aa overlap (20-150:254-384) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF ::::::::.::::::::::::::::::::: gi|126 ILHLDLKPENILCVSRDAKQIKIIDFGLARRYKPREKLRVNFGTPEFLAPEVVNYDFVSF 230 240 250 260 270 280 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL ::::::::::::::::::::::::.:::::.::::: ::::.:::: ::::::::::::: gi|126 PTDMWSVGVIAYMLLSGLSPFLGDSDAETLSNILACSWDLEEEEFQGISEEAKEFISKLL 290 300 310 320 330 340 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::.:: :::.:::: :: :: gi|126 IKEKSWRISASEALKHPWLSDHNLHRRLNSQKKKIPGSKAQ 350 360 370 380 >>gi|109505717|ref|XP_001066672.1| PREDICTED: similar to (578 aa) initn: 809 init1: 787 opt: 787 Z-score: 946.2 bits: 183.1 E(): 5.3e-44 Smith-Waterman score: 787; 95.902% identity (99.180% similar) in 122 aa overlap (20-141:299-420) 10 20 30 40 FLJ002 QCSRFLIDTSILPLCLLNCRYKPREKLKVNFGTPEFLAPEVVNYDFVSF :::::::::::::::::::::::::::::: gi|109 ILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYDFVSF 270 280 290 300 310 320 50 60 70 80 90 100 FLJ002 PTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLL :::::::::.:::::::::::::::::::.::::::::::::::::::::::::::::: gi|109 ATDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQDISEEAKEFISKLL 330 340 350 360 370 380 110 120 130 140 150 FLJ002 IKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK ::::::::::::::::::::::::::::.::. gi|109 IKEKSWRISASEALKHPWLSDHKLHSRLSAQENCCGCCCFEKAFGKISSSGALTPVMTGS 390 400 410 420 430 440 gi|109 PNMGSLELELGEALLCSAVRTLKNHDRHSVLRGAINGHDSRHDLSFEDLTADTDGIEGHR 450 460 470 480 490 500 155 residues in 1 query sequences 2693465022 residues in 7827732 library sequences Tcomplib [34.26] (2 proc) start: Sat Feb 28 03:53:44 2009 done: Sat Feb 28 03:57:50 2009 Total Scan time: 596.120 Total Display time: 0.030 Function used was FASTA [version 34.26.5 April 26, 2007]