##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I Aor51HI
ApaLI AseI AsnI Asp718I AviII
BclI BglII BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SacII SalI SapI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 4390 GACGTC GACGT^C
AccI 315 GTCTAC GT^[AC][GT]AC
AccII 1489 CGCG CG^CG
AcsI 1719 AAATTT [GA]^AATT[CT]
AflII 3032 CTTAAG C^TTAAG
AhdI 1027 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1719 AAATTT [GA]^AATT[CT]
AscI 1487 GGCGCGCC GG^CGCGCC
Asp700I 1254 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1027 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1455 GGATCC G^GATCC
BbrPI 151 CACGTG CAC^GTG
BcgI 347 CGAGGCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3032 CTTAAG C^TTAAG
BglI 2392 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 3923 CGGCCG CG[GA][CT]^CG
BspHI 4157 TCATGA T^CATGA
BspLU11I 4286 ACATGT A^CATGT
BssHII 1488 GCGCGC G^CGCGC
BstUI 1489 CGCG CG^CG
EagI 3923 CGGCCG C^GGCCG
Eam1105I 1027 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3923 CGGCCG C^GGCCG
Eco52I 3923 CGGCCG C^GGCCG
HincII 1439 GTTGAC GT[CT]^[GA]AC
HindII 1439 GTTGAC GT[CT]^[GA]AC
MvnI 1489 CGCG CG^CG
NaeI 1193 GCCGGC GCC^GGC
NgoMIV 1193 GCCGGC G^CCGGC
NheI 2065 GCTAGC G^CTAGC
PmaCI 151 CACGTG CAC^GTG
PmlI 151 CACGTG CAC^GTG
PshAI 1706 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4157 TCATGA T^CATGA
ScaI 3273 AGTACT AGT^ACT
SexAI 730 ACCAGGT A^CC[AT]GGT
SphI 2886 GCATGC GCATG^C
XmnI 1254 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 3012 AACGTT AA^CGTT
4487 AACGTT
AflIII 1012 ACGTGT A^C[GA][CT]GT
4286 ACATGT
AspI 2353 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4352 GACTGTGTC
BbeI 350 GGCGCC GGCGC^C
1780 GGCGCC
BciVI 1612 GGATAC GTATCC(6/5)
2239 GGATAC
BsaAI 151 CACGTG [CT]AC^GT[GA]
3647 CACGTA
BsrBI 291 CCGCTC CCGCTC(-3/-3)
1122 CCGCTC
BsrDI 173 CATTGC GCAATG(2/0)
556 GCAATG
BssSI 3285 CACGAG CACGAG(-5/-1)
3200 CTCGTG
BstAPI 810 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1987 GCACTACCTGC
BstEII 1712 GGTCACC G^GT[ACGT]ACC
3944 GGTCACC
BstPI 1712 GGTCACC G^GT[ACGT]ACC
3944 GGTCACC
BstXI 1365 CCAGTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3186 CCACCTGAGTGG
EcoO65I 1712 GGTCACC G^GT[ACGT]ACC
3944 GGTCACC
KasI 350 GGCGCC G^GCGCC
1780 GGCGCC
NarI 350 GGCGCC GG^CGCC
1780 GGCGCC
NcoI 1350 CCATGG C^CATGG
1483 CCATGG
PflFI 2353 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4352 GACTGTGTC
PflMI 217 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1938 CCAAGAGTTGG
Psp1406I 3012 AACGTT AA^CGTT
4487 AACGTT
SbfI 4031 CCTGCAGG CCTGCA^GG
4058 CCTGCAGG
SfoI 350 GGCGCC GGC^GCC
1780 GGCGCC
SmaI 1077 CCCGGG CCC^GGG
1567 CCCGGG
Sse8387I 4031 CCTGCAGG CCTGCA^GG
4058 CCTGCAGG
Tth111I 2353 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4352 GACTGTGTC
Van91I 217 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1938 CCAAGAGTTGG
XcmI 2426 CCAGTGTGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3284 CCACGAGCCCTCTGG
XmaCI 1077 CCCGGG C^CCGGG
1567 CCCGGG
XmaI 1077 CCCGGG C^CCGGG
1567 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII