##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BbeI BciVI BfrI BglII
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI Bst1107I BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2437 GTCTAC GT^[AC][GT]AC
AcsI 4684 AAATTT [GA]^AATT[CT]
ApoI 4684 AAATTT [GA]^AATT[CT]
BbrPI 1616 CACGTG CAC^GTG
BcgI 3810 GCAGCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 4136 CGTCTC CGTCTC(1/5)
Bsp1407I 3948 TGTACA T^GTACA
BspLU11I 722 ACATGT A^CATGT
BsrDI 205 CATTGC GCAATG(2/0)
BsrGI 3948 TGTACA T^GTACA
BssHII 489 GCGCGC G^CGCGC
DraI 861 TTTAAA TTT^AAA
DrdI 574 GACCCCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 3444 AAGCTT A^AGCTT
HpaI 10 GTTAAC GTT^AAC
PaeR7I 691 CTCGAG C^TCGAG
PmaCI 1616 CACGTG CAC^GTG
PmlI 1616 CACGTG CAC^GTG
PshAI 4504 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 870 ACCTGGT A^CC[AT]GGT
SmaI 2646 CCCGGG CCC^GGG
SspBI 3948 TGTACA T^GTACA
Tsp509I 4685 AATT ^AATT
XbaI 1790 TCTAGA T^CTAGA
XhoI 691 CTCGAG C^TCGAG
XmaCI 2646 CCCGGG C^CCGGG
XmaI 2646 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 3651 GACGCC G[GA]^CG[CT]C
4416 GGCGTC
AflIII 722 ACATGT A^C[GA][CT]GT
1617 ACGTGT
AvrII 2272 CCTAGG C^CTAGG
3336 CCTAGG
BamHI 2120 GGATCC G^GATCC
4424 GGATCC
BclI 1128 TGATCA T^GATCA
1940 TGATCA
BlnI 2272 CCTAGG C^CTAGG
3336 CCTAGG
BlpI 497 GCTGAGC GC^T[ACGT]AGC
4385 GCTGAGC
Bpu1102I 497 GCTGAGC GC^T[ACGT]AGC
4385 GCTGAGC
BsaAI 1616 CACGTG [CT]AC^GT[GA]
2838 CACGTA
BsaHI 3651 GACGCC G[GA]^CG[CT]C
4416 GGCGTC
BsaI 3420 GAGACC GGTCTC(1/5)
1798 GGTCTC
BspHI 1035 TCATGA T^CATGA
1627 TCATGA
BssSI 2377 CACGAG CACGAG(-5/-1)
4139 CTCGTG
BstYI 2120 GGATCC [GA]^GATC[CT]
4424 GGATCC
CelII 497 GCTGAGC GC^T[ACGT]AGC
4385 GCTGAGC
DraIII 1282 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3191 CACCCTGTG
FbaI 1128 TGATCA T^GATCA
1940 TGATCA
Hin1I 3651 GACGCC G[GA]^CG[CT]C
4416 GGCGTC
HincII 10 GTTAAC GT[CT]^[GA]AC
2060 GTCAAC
HindII 10 GTTAAC GT[CT]^[GA]AC
2060 GTCAAC
MflI 2120 GGATCC [GA]^GATC[CT]
4424 GGATCC
NaeI 493 GCCGGC GCC^GGC
4434 GCCGGC
NgoMIV 493 GCCGGC G^CCGGC
4434 GCCGGC
PflMI 1512 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4351 CCACCAGCTGG
RcaI 1035 TCATGA T^CATGA
1627 TCATGA
SapI 1180 GAAGAGC GCTCTTC(1/4)
4486 GAAGAGC
TfiI 2581 GAATC G^A[AT]TC
4215 GATTC
Van91I 1512 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4351 CCACCAGCTGG
XhoII 2120 GGATCC [GA]^GATC[CT]
4424 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI