##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI ApoI AscI AseI
AsnI AspEI AviII BcgI BciVI
BfrI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I EcoRI EcoT22I
FseI FspI HpaI KspI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1022 GACGTC GACGT^C
Acc65I 3891 GGTACC G^GTACC
AccI 4417 GTCTAC GT^[AC][GT]AC
Aor51HI 1002 AGCGCT AGC^GCT
Asp700I 1580 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3891 GGTACC G^GTACC
BamHI 777 GGATCC G^GATCC
BclI 1317 TGATCA T^GATCA
BglII 3508 AGATCT A^GATCT
BlpI 192 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 192 GCTCAGC GC^T[ACGT]AGC
BsaWI 1164 ACCGGA [AT]^CCGG[AT]
BsiEI 3778 CGGCCG CG[GA][CT]^CG
BsrBI 3694 GAGCGG CCGCTC(-3/-3)
BssSI 1702 CACGAG CACGAG(-5/-1)
CelII 192 GCTCAGC GC^T[ACGT]AGC
DrdI 545 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3778 CGGCCG C^GGCCG
EclXI 3778 CGGCCG C^GGCCG
Eco47III 1002 AGCGCT AGC^GCT
Eco52I 3778 CGGCCG C^GGCCG
EcoRV 1487 GATATC GAT^ATC
FbaI 1317 TGATCA T^GATCA
HincII 1298 GTCAAC GT[CT]^[GA]AC
HindII 1298 GTCAAC GT[CT]^[GA]AC
KpnI 3891 GGTACC GGTAC^C
NcoI 3955 CCATGG C^CATGG
PaeR7I 1763 CTCGAG C^TCGAG
PshAI 2928 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4103 GAAGAGC GCTCTTC(1/4)
SfiI 3092 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3979 CCCGGG CCC^GGG
SwaI 3044 ATTTAAAT ATTT^AAAT
Tsp509I 3043 AATT ^AATT
XhoI 1763 CTCGAG C^TCGAG
XmaCI 3979 CCCGGG C^CCGGG
XmaI 3979 CCCGGG C^CCGGG
XmnI 1580 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 2169 GTGCAC G^TGCAC
3262 GTGCAC
ApaLI 2169 GTGCAC G^TGCAC
3262 GTGCAC
AspI 338 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1292 GACATTGTC
BbeI 102 GGCGCC GGCGC^C
1652 GGCGCC
BbrPI 991 CACGTG CAC^GTG
3269 CACGTG
BbvCI 121 CCTCAGC CCTCAGC(-5/-2)
4120 GCTGAGG
BsaAI 991 CACGTG [CT]AC^GT[GA]
3269 CACGTG
BsaBI 1459 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1585 GATTCAGATC
BsrDI 244 CATTGC GCAATG(2/0)
685 CATTGC
DraI 1016 TTTAAA TTT^AAA
3045 TTTAAA
KasI 102 GGCGCC G^GCGCC
1652 GGCGCC
MamI 1459 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1585 GATTCAGATC
NarI 102 GGCGCC GG^CGCC
1652 GGCGCC
NdeI 3384 CATATG CA^TATG
4300 CATATG
PflFI 338 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1292 GACATTGTC
PmaCI 991 CACGTG CAC^GTG
3269 CACGTG
PmlI 991 CACGTG CAC^GTG
3269 CACGTG
SbfI 3451 CCTGCAGG CCTGCA^GG
3757 CCTGCAGG
SexAI 744 ACCAGGT A^CC[AT]GGT
2260 ACCTGGT
SfoI 102 GGCGCC GGC^GCC
1652 GGCGCC
Sse8387I 3451 CCTGCAGG CCTGCA^GG
3757 CCTGCAGG
StuI 1471 AGGCCT AGG^CCT
2300 AGGCCT
TfiI 1585 GATTC G^A[AT]TC
4435 GAATC
Tth111I 338 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1292 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII