##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AviII AvrII BbrPI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsiWI BsmI BspDI
BspEI BspHI BsrBI BstBI BstEII
BstPI CelII ClaI CpoI DrdI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII MluI
MroI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 308 GGCGCC G[GA]^CG[CT]C
ApaI 2098 GGGCCC GGGCC^C
Asp700I 4047 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 308 GGCGCC GGCGC^C
BbvCI 1033 GCTGAGG CCTCAGC(-5/-2)
BsaHI 308 GGCGCC G[GA]^CG[CT]C
BsaWI 2953 TCCGGT [AT]^CCGG[AT]
BsrFI 1932 GCCGGC [GA]^CCGG[CT]
BssSI 3005 CTCGTG CACGAG(-5/-1)
Bst1107I 3018 GTATAC GTA^TAC
BstAPI 4002 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3018 GTATAC GTA^TAC
Cfr10I 1932 GCCGGC [GA]^CCGG[CT]
EagI 148 CGGCCG C^GGCCG
EclXI 148 CGGCCG C^GGCCG
Eco52I 148 CGGCCG C^GGCCG
HaeII 308 GGCGCC [GA]GCGC^[CT]
HgaI 179 GACGC GACGC(5/10)
Hin1I 308 GGCGCC G[GA]^CG[CT]C
HincII 728 GTTAAC GT[CT]^[GA]AC
HindII 728 GTTAAC GT[CT]^[GA]AC
HpaI 728 GTTAAC GTT^AAC
KasI 308 GGCGCC G^GCGCC
MaeII 4034 ACGT A^CGT
MfeI 1697 CAATTG C^AATTG
MunI 1697 CAATTG C^AATTG
NaeI 1932 GCCGGC GCC^GGC
NarI 308 GGCGCC GG^CGCC
NdeI 4620 CATATG CA^TATG
NgoMIV 1932 GCCGGC G^CCGGC
NheI 390 GCTAGC G^CTAGC
PflMI 3309 CCACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3902 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 596 GAAGAGC GCTCTTC(1/4)
SbfI 2193 CCTGCAGG CCTGCA^GG
SfoI 308 GGCGCC GGC^GCC
SmaI 41 CCCGGG CCC^GGG
Sse8387I 2193 CCTGCAGG CCTGCA^GG
Van91I 3309 CCACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
XmnI 4047 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1826 GGTACC G^GTACC
4615 GGTACC
AflIII 2803 ACATGT A^C[GA][CT]GT
4111 ACATGT
AlwNI 1122 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
3840 CAGTATCTG
Asp718I 1826 GGTACC G^GTACC
4615 GGTACC
AvaI 41 CCCGGG C^[CT]CG[GA]G
64 CTCGGG
BamHI 1877 GGATCC G^GATCC
2691 GGATCC
BbsI 1583 GAAGAC GAAGAC(2/6)
1770 GTCTTC
BcgI 1269 CGAGGACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2997 GCAATGGACTCG
BglI 11 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2126 GCCCTGTTGGC
BpuAI 1583 GAAGAC GAAGAC(2/6)
1770 GTCTTC
BsiEI 1 CGACCG CG[GA][CT]^CG
148 CGGCCG
BsoBI 41 CCCGGG C^[CT]CG[GA]G
64 CTCGGG
Bsp1407I 1525 TGTACA T^GTACA
4675 TGTACA
BspLU11I 2803 ACATGT A^CATGT
4111 ACATGT
BsrDI 585 GCAATG GCAATG(2/0)
2997 GCAATG
BsrGI 1525 TGTACA T^GTACA
4675 TGTACA
BstXI 894 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1184 CCAGGTACATGG
BstYI 1877 GGATCC [GA]^GATC[CT]
2691 GGATCC
DraI 430 TTTAAA TTT^AAA
2978 TTTAAA
EcoRI 1612 GAATTC G^AATTC
2262 GAATTC
KpnI 1826 GGTACC GGTAC^C
4615 GGTACC
KspI 4 CCGCGG CCGC^GG
145 CCGCGG
MflI 1877 GGATCC [GA]^GATC[CT]
2691 GGATCC
MslI 1958 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4507 CACCAATGTG
SacI 2430 GAGCTC GAGCT^C
4024 GAGCTC
SacII 4 CCGCGG CCGC^GG
145 CCGCGG
SexAI 1398 ACCTGGT A^CC[AT]GGT
4432 ACCTGGT
SphI 253 GCATGC GCATG^C
1787 GCATGC
SspBI 1525 TGTACA T^GTACA
4675 TGTACA
XbaI 1608 TCTAGA T^CTAGA
3356 TCTAGA
XcmI 2236 CCATCTGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3753 CCATCCTTACGATGG
XhoII 1877 GGATCC [GA]^GATC[CT]
2691 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AocI ApaLI
ApoI AspHI AspI AvaII BalI
BanI BanII BbvI BciVI BcnI
BfaI BmyI BpmI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrI BssHII BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I