##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AspI
BamHI BbrPI BciVI BglII BsaBI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoT22I FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 2850 CTTAAG C^TTAAG
AflIII 87 ACACGT A^C[GA][CT]GT
AvrII 1697 CCTAGG C^CTAGG
BclI 802 TGATCA T^GATCA
BfrI 2850 CTTAAG C^TTAAG
BlnI 1697 CCTAGG C^CTAGG
BlpI 2473 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2473 GCTGAGC GC^T[ACGT]AGC
BsmBI 272 CGTCTC CGTCTC(1/5)
BsmI 99 GAATGC GAATGC(1/-1)
BsrDI 1109 CATTGC GCAATG(2/0)
BstBI 2862 TTCGAA TT^CGAA
BstYI 248 GGATCT [GA]^GATC[CT]
CelII 2473 GCTGAGC GC^T[ACGT]AGC
DraIII 237 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 802 TGATCA T^GATCA
KspI 3976 CCGCGG CCGC^GG
MflI 248 GGATCT [GA]^GATC[CT]
MseI 2851 TTAA T^TAA
NspV 2862 TTCGAA TT^CGAA
SacII 3976 CCGCGG CCGC^GG
SbfI 2503 CCTGCAGG CCTGCA^GG
ScaI 2776 AGTACT AGT^ACT
SexAI 2090 ACCAGGT A^CC[AT]GGT
SfiI 2846 GGCCCTTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2862 TTCGAA TT^CGAA
SgrAI 1986 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2503 CCTGCAGG CCTGCA^GG
TfiI 4 GAATC G^A[AT]TC
Tru9I 2851 TTAA T^TAA
Tsp509I 1404 AATT ^AATT
XhoII 248 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 3520 GGTACC G^GTACC
4045 GGTACC
Alw44I 1335 GTGCAC G^TGCAC
1802 GTGCAC
ApaI 364 GGGCCC GGGCC^C
544 GGGCCC
ApaLI 1335 GTGCAC G^TGCAC
1802 GTGCAC
Asp718I 3520 GGTACC G^GTACC
4045 GGTACC
BalI 1036 TGGCCA TGG^CCA
3443 TGGCCA
BbeI 1990 GGCGCC GGCGC^C
2898 GGCGCC
BbsI 60 GAAGAC GAAGAC(2/6)
2398 GAAGAC
BbvCI 2493 CCTCAGC CCTCAGC(-5/-2)
2208 GCTGAGG
BglI 2847 GCCCTTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3406 GCCAGAAGGGC
BpuAI 60 GAAGAC GAAGAC(2/6)
2398 GAAGAC
BsaAI 88 CACGTA [CT]AC^GT[GA]
1965 TACGTG
BsaI 432 GAGACC GGTCTC(1/5)
3079 GAGACC
DrdI 717 GACCAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2086 GACTACCAGGTC
EcoRV 993 GATATC GAT^ATC
3982 GATATC
KasI 1990 GGCGCC G^GCGCC
2898 GGCGCC
KpnI 3520 GGTACC GGTAC^C
4045 GGTACC
MluNI 1036 TGGCCA TGG^CCA
3443 TGGCCA
MscI 1036 TGGCCA TGG^CCA
3443 TGGCCA
NarI 1990 GGCGCC GG^CGCC
2898 GGCGCC
SfoI 1990 GGCGCC GGC^GCC
2898 GGCGCC
StuI 1318 AGGCCT AGG^CCT
3764 AGGCCT
XcmI 1774 CCATCCTGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1999 CCAAGGCCCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII AviII BanI BanII BbvI
BcgI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XmaCI
XmaI