##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI BclI BfrI BglII BsaBI
BsiWI BspDI Bst1107I BstBI BstZ17I
ClaI Eam1105I EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI ScaI SfuI SmlI SnaBI
SpeI SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 38 GTCGAC GT^[AC][GT]AC
AgeI 2274 ACCGGT A^CCGGT
Aor51HI 4200 AGCGCT AGC^GCT
AspI 561 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1682 TGCGCA TGC^GCA
BamHI 3663 GGATCC G^GATCC
BbrPI 2028 CACGTG CAC^GTG
BciVI 345 GTATCC GTATCC(6/5)
Bsp1407I 4468 TGTACA T^GTACA
BspHI 54 TCATGA T^CATGA
BspLU11I 905 ACATGT A^CATGT
BsrBI 4034 CCGCTC CCGCTC(-3/-3)
BsrDI 1520 GCAATG GCAATG(2/0)
BsrGI 4468 TGTACA T^GTACA
BssHII 4321 GCGCGC G^CGCGC
BstEII 3597 GGTGACC G^GT[ACGT]ACC
BstPI 3597 GGTGACC G^GT[ACGT]ACC
CpoI 559 CGGACCG CG^G[AT]CCG
DraI 4543 TTTAAA TTT^AAA
DraIII 1686 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3824 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4200 AGCGCT AGC^GCT
EcoO65I 3597 GGTGACC G^GT[ACGT]ACC
EcoRI 3443 GAATTC G^AATTC
FspI 1682 TGCGCA TGC^GCA
HincII 38 GTCGAC GT[CT]^[GA]AC
HindII 38 GTCGAC GT[CT]^[GA]AC
NaeI 4388 GCCGGC GCC^GGC
NgoMIV 4388 GCCGGC G^CCGGC
PflFI 561 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2274 ACCGGT A^CCGGT
PmaCI 2028 CACGTG CAC^GTG
PmlI 2028 CACGTG CAC^GTG
RcaI 54 TCATGA T^CATGA
RsrII 559 CGGACCG CG^G[AT]CCG
SalI 38 GTCGAC G^TCGAC
SapI 301 GAAGAGC GCTCTTC(1/4)
SexAI 1687 ACCAGGT A^CC[AT]GGT
SgrAI 2273 CACCGGTG C[GA]^CCGG[CT]G
SspBI 4468 TGTACA T^GTACA
Tth111I 561 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 361 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 2099 TCCGGA T^CCGGA
3839 TCCGGA
AcsI 3443 GAATTC [GA]^AATT[CT]
4547 AAATTT
AflIII 905 ACATGT A^C[GA][CT]GT
2029 ACGTGT
AocI 184 CCTCAGG CC^T[ACGT]AGG
1942 CCTGAGG
ApoI 3443 GAATTC [GA]^AATT[CT]
4547 AAATTT
BbsI 4075 GAAGAC GAAGAC(2/6)
1426 GTCTTC
BpuAI 4075 GAAGAC GAAGAC(2/6)
1426 GTCTTC
BsaI 948 GAGACC GGTCTC(1/5)
2318 GAGACC
BseAI 2099 TCCGGA T^CCGGA
3839 TCCGGA
BsmBI 1458 CGTCTC CGTCTC(1/5)
4128 CGTCTC
BsmI 173 GAATGC GAATGC(1/-1)
3552 GCATTC
BspEI 2099 TCCGGA T^CCGGA
3839 TCCGGA
BssSI 3175 CACGAG CACGAG(-5/-1)
462 CTCGTG
Bsu36I 184 CCTCAGG CC^T[ACGT]AGG
1942 CCTGAGG
Eco81I 184 CCTCAGG CC^T[ACGT]AGG
1942 CCTGAGG
MroI 2099 TCCGGA T^CCGGA
3839 TCCGGA
SbfI 1790 CCTGCAGG CCTGCA^GG
2701 CCTGCAGG
SfiI 2110 GGCCCATGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2883 GGCCAGGGAGGCC
Sse8387I 1790 CCTGCAGG CCTGCA^GG
2701 CCTGCAGG
TfiI 2212 GATTC G^A[AT]TC
2986 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbeI BbvCI BbvI
BcgI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SphI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI