##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccI AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII BciVI BglII
BsiWI BsmI BspDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KspI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SacII SalI SapI ScaI SfuI
SgrAI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2538 GACGTC GACGT^C
Acc65I 1131 GGTACC G^GTACC
AcsI 1772 GAATTT [GA]^AATT[CT]
AflII 2637 CTTAAG C^TTAAG
AocI 1383 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2036 AGCGCT AGC^GCT
ApoI 1772 GAATTT [GA]^AATT[CT]
Asp718I 1131 GGTACC G^GTACC
BbrPI 2553 CACGTG CAC^GTG
BcgI 1879 GCACGATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 269 TGATCA T^GATCA
BfrI 2637 CTTAAG C^TTAAG
BsaAI 2553 CACGTG [CT]AC^GT[GA]
BsaBI 2018 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 1834 ACATGT A^CATGT
BsrDI 41 CATTGC GCAATG(2/0)
Bsu36I 1383 CCTTAGG CC^T[ACGT]AGG
Eco47III 2036 AGCGCT AGC^GCT
Eco81I 1383 CCTTAGG CC^T[ACGT]AGG
FbaI 269 TGATCA T^GATCA
KpnI 1131 GGTACC GGTAC^C
MamI 2018 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1533 GCCGGC GCC^GGC
NgoMIV 1533 GCCGGC G^CCGGC
PmaCI 2553 CACGTG CAC^GTG
PmlI 2553 CACGTG CAC^GTG
PvuI 1882 CGATCG CGAT^CG
SacI 2054 GAGCTC GAGCT^C
SbfI 579 CCTGCAGG CCTGCA^GG
SphI 1214 GCATGC GCATG^C
Sse8387I 579 CCTGCAGG CCTGCA^GG
TaqI 2686 TCGA T^CGA
TthHB8I 2686 TCGA T^CGA
XcmI 3235 CCATCTTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 2542 TCCGGA T^CCGGA
3274 TCCGGA
AvrII 1644 CCTAGG C^CTAGG
2861 CCTAGG
BamHI 3355 GGATCC G^GATCC
3858 GGATCC
BlnI 1644 CCTAGG C^CTAGG
2861 CCTAGG
BsaWI 2542 TCCGGA [AT]^CCGG[AT]
3274 TCCGGA
BseAI 2542 TCCGGA T^CCGGA
3274 TCCGGA
Bsp1407I 1809 TGTACA T^GTACA
2708 TGTACA
BspEI 2542 TCCGGA T^CCGGA
3274 TCCGGA
BsrBI 836 CCGCTC CCGCTC(-3/-3)
3102 GAGCGG
BsrGI 1809 TGTACA T^GTACA
2708 TGTACA
DraIII 2508 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3141 CACCATGTG
EagI 3316 CGGCCG C^GGCCG
3600 CGGCCG
EarI 3213 CTCTTC CTCTTC(1/4)
3792 GAAGAG
EclXI 3316 CGGCCG C^GGCCG
3600 CGGCCG
Eco52I 3316 CGGCCG C^GGCCG
3600 CGGCCG
EcoNI 1454 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1958 CCTGCTGCAGG
Ksp632I 3213 CTCTTC CTCTTC(1/4)
3792 GAAGAG
MroI 2542 TCCGGA T^CCGGA
3274 TCCGGA
MslI 51 CACGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4053 CACTGGGGTG
NspI 1214 GCATGC [GA]CATG^[CT]
1834 ACATGT
SexAI 2251 ACCTGGT A^CC[AT]GGT
2509 ACCAGGT
SspBI 1809 TGTACA T^GTACA
2708 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmaCI XmaI