##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII BclI BfrI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI DraIII Eam1105I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI SalI SapI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 4296 AAATTT [GA]^AATT[CT]
AflIII 393 ACGCGT A^C[GA][CT]GT
Alw44I 2304 GTGCAC G^TGCAC
Aor51HI 2163 AGCGCT AGC^GCT
ApaLI 2304 GTGCAC G^TGCAC
ApoI 4296 AAATTT [GA]^AATT[CT]
BamHI 2685 GGATCC G^GATCC
BbrPI 1052 CACGTG CAC^GTG
BcgI 2732 CGAATTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1657 GTATCC GTATCC(6/5)
BglII 3222 AGATCT A^GATCT
BsaAI 1052 CACGTG [CT]AC^GT[GA]
BsmBI 1759 CGTCTC CGTCTC(1/5)
BsmI 4054 GCATTC GAATGC(1/-1)
BssSI 2307 CACGAG CACGAG(-5/-1)
BstEII 3310 GGTGACC G^GT[ACGT]ACC
BstPI 3310 GGTGACC G^GT[ACGT]ACC
CpoI 1809 CGGACCG CG^G[AT]CCG
DraI 4285 TTTAAA TTT^AAA
DrdI 651 GACGGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2163 AGCGCT AGC^GCT
EcoNI 1828 CCTCGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3310 GGTGACC G^GT[ACGT]ACC
EcoT22I 4028 ATGCAT ATGCA^T
MluI 393 ACGCGT A^CGCGT
NsiI 4028 ATGCAT ATGCA^T
NspI 3957 ACATGC [GA]CATG^[CT]
PmaCI 1052 CACGTG CAC^GTG
PmlI 1052 CACGTG CAC^GTG
PshAI 1479 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1809 CGGACCG CG^G[AT]CCG
SbfI 3259 CCTGCAGG CCTGCA^GG
ScaI 1686 AGTACT AGT^ACT
SfiI 2778 GGCCCCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3259 CCTGCAGG CCTGCA^GG
XbaI 3301 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2925 GTAGAC GT^[AC][GT]AC
3329 GTAGAC
AfaI 1687 GTAC GT^AC
4255 GTAC
AocI 1951 CCTCAGG CC^T[ACGT]AGG
3159 CCTTAGG
AspI 1544 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3136 GACACTGTC
BbeI 14 GGCGCC GGCGC^C
1075 GGCGCC
BlpI 831 GCTCAGC GC^T[ACGT]AGC
929 GCTGAGC
Bpu1102I 831 GCTCAGC GC^T[ACGT]AGC
929 GCTGAGC
BsiEI 1651 CGGCCG CG[GA][CT]^CG
1990 CGGCCG
Bsu36I 1951 CCTCAGG CC^T[ACGT]AGG
3159 CCTTAGG
CelII 831 GCTCAGC GC^T[ACGT]AGC
929 GCTGAGC
EagI 1651 CGGCCG C^GGCCG
1990 CGGCCG
EclXI 1651 CGGCCG C^GGCCG
1990 CGGCCG
Eco52I 1651 CGGCCG C^GGCCG
1990 CGGCCG
Eco81I 1951 CCTCAGG CC^T[ACGT]AGG
3159 CCTTAGG
KasI 14 GGCGCC G^GCGCC
1075 GGCGCC
NarI 14 GGCGCC GG^CGCC
1075 GGCGCC
NheI 149 GCTAGC G^CTAGC
2215 GCTAGC
PflFI 1544 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3136 GACACTGTC
PvuII 1727 CAGCTG CAG^CTG
3806 CAGCTG
RsaI 1687 GTAC GT^AC
4255 GTAC
SfoI 14 GGCGCC GGC^GCC
1075 GGCGCC
SmaI 3363 CCCGGG CCC^GGG
3395 CCCGGG
Tsp509I 2734 AATT ^AATT
4297 AATT
Tth111I 1544 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3136 GACACTGTC
XcmI 3372 CCATGGAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3760 CCAGTGCCACACTGG
XmaCI 3363 CCCGGG C^CCGGG
3395 CCCGGG
XmaI 3363 CCCGGG C^CCGGG
3395 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI SacI SacII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmnI