##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI BamHI BclI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BspLU11I Bst1107I BstBI BstZ17I
ClaI Eam1105I Eco47III EcoT22I FbaI
FseI HpaI MamI MluI MroI
NaeI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI Sse8387I SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
BglII 1022 AGATCT A^GATCT
BspHI 1762 TCATGA T^CATGA
BsrFI 491 GCCGGT [GA]^CCGG[CT]
BstAPI 1535 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 491 GCCGGT [GA]^CCGG[CT]
DraI 4476 TTTAAA TTT^AAA
EcoRI 691 GAATTC G^AATTC
EcoRV 4570 GATATC GAT^ATC
HincII 4254 GTCAAC GT[CT]^[GA]AC
HindII 4254 GTCAAC GT[CT]^[GA]AC
KspI 369 CCGCGG CCGC^GG
NdeI 3552 CATATG CA^TATG
NheI 49 GCTAGC G^CTAGC
RcaI 1762 TCATGA T^CATGA
SacII 369 CCGCGG CCGC^GG
SmaI 2754 CCCGGG CCC^GGG
SphI 641 GCATGC GCATG^C
TfiI 3354 GATTC G^A[AT]TC
XmaCI 2754 CCCGGG C^CCGGG
XmaI 2754 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3876 GGTACC G^GTACC
3996 GGTACC
AcsI 691 GAATTC [GA]^AATT[CT]
3175 AAATTC
AcyI 1442 GGCGCC G[GA]^CG[CT]C
4391 GGCGCC
AflIII 658 ACGTGT A^C[GA][CT]GT
2084 ACGTGT
Alw44I 561 GTGCAC G^TGCAC
2076 GTGCAC
ApaLI 561 GTGCAC G^TGCAC
2076 GTGCAC
ApoI 691 GAATTC [GA]^AATT[CT]
3175 AAATTC
Asp718I 3876 GGTACC G^GTACC
3996 GGTACC
BbeI 1442 GGCGCC GGCGC^C
4391 GGCGCC
BbrPI 657 CACGTG CAC^GTG
2083 CACGTG
BcgI 603 CGAGACACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4340 CGACCTGCCTGC
BciVI 708 GTATCC GTATCC(6/5)
3407 GTATCC
BsaAI 657 CACGTG [CT]AC^GT[GA]
2083 CACGTG
BsaHI 1442 GGCGCC G[GA]^CG[CT]C
4391 GGCGCC
BsaWI 3218 ACCGGA [AT]^CCGG[AT]
3708 TCCGGT
BsmBI 1679 CGTCTC CGTCTC(1/5)
2484 CGTCTC
BssSI 601 CACGAG CACGAG(-5/-1)
347 CTCGTG
BstXI 1900 CCAGGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2198 CCAGGTGAATGG
BstYI 1022 AGATCT [GA]^GATC[CT]
3705 AGATCC
CpoI 455 CGGACCG CG^G[AT]CCG
493 CGGTCCG
DrdI 619 GACCGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3842 GACCTGCGTGTC
EagI 372 CGGCCG C^GGCCG
3243 CGGCCG
EclXI 372 CGGCCG C^GGCCG
3243 CGGCCG
Eco52I 372 CGGCCG C^GGCCG
3243 CGGCCG
HgaI 2012 GACGC GACGC(5/10)
1678 GCGTC
Hin1I 1442 GGCGCC G[GA]^CG[CT]C
4391 GGCGCC
KasI 1442 GGCGCC G^GCGCC
4391 GGCGCC
KpnI 3876 GGTACC GGTAC^C
3996 GGTACC
MfeI 2331 CAATTG C^AATTG
2688 CAATTG
MflI 1022 AGATCT [GA]^GATC[CT]
3705 AGATCC
MunI 2331 CAATTG C^AATTG
2688 CAATTG
NarI 1442 GGCGCC GG^CGCC
4391 GGCGCC
PmaCI 657 CACGTG CAC^GTG
2083 CACGTG
PmlI 657 CACGTG CAC^GTG
2083 CACGTG
PstI 611 CTGCAG CTGCA^G
4037 CTGCAG
RsrII 455 CGGACCG CG^G[AT]CCG
493 CGGTCCG
SexAI 2955 ACCAGGT A^CC[AT]GGT
3981 ACCAGGT
SfoI 1442 GGCGCC GGC^GCC
4391 GGCGCC
SpeI 1735 ACTAGT A^CTAGT
4052 ACTAGT
XhoII 1022 AGATCT [GA]^GATC[CT]
3705 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssHII BssKI
BstEII BstF5I BstNI BstPI BstUI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI