##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII Alw44I ApaLI AscI
Asp700I AviII BbrPI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI BstBI
ClaI CpoI EagI EclXI Eco52I
EcoRV FseI FspI HincII HindII
HpaI KspI MfeI MroI MunI
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 4116 GGTACC G^GTACC
AclI 2099 AACGTT AA^CGTT
AflII 122 CTTAAG C^TTAAG
AgeI 4321 ACCGGT A^CCGGT
AhdI 2692 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4499 AGCGCT AGC^GCT
ApaI 4007 GGGCCC GGGCC^C
Asp718I 4116 GGTACC G^GTACC
AspEI 2692 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2349 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 568 CTCGAG C^[CT]CG[GA]G
BbeI 1797 GGCGCC GGCGC^C
BbvCI 2259 GCTGAGG CCTCAGC(-5/-2)
BcgI 4511 GCACACCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4838 TGATCA T^GATCA
BfrI 122 CTTAAG C^TTAAG
BglII 683 AGATCT A^GATCT
BlpI 4261 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4261 GCTCAGC GC^T[ACGT]AGC
BsaBI 4174 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 568 CTCGAG C^[CT]CG[GA]G
BspLU11I 2825 ACATGT A^CATGT
BspMI 2717 ACCTGC ACCTGC(4/8)
Bst1107I 4389 GTATAC GTA^TAC
BstEII 2847 GGTGACC G^GT[ACGT]ACC
BstPI 2847 GGTGACC G^GT[ACGT]ACC
BstZ17I 4389 GTATAC GTA^TAC
CelII 4261 GCTCAGC GC^T[ACGT]AGC
DraIII 1346 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2692 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4499 AGCGCT AGC^GCT
EcoO65I 2847 GGTGACC G^GT[ACGT]ACC
EcoRI 1997 GAATTC G^AATTC
EcoT22I 277 ATGCAT ATGCA^T
FbaI 4838 TGATCA T^GATCA
KasI 1797 GGCGCC G^GCGCC
KpnI 4116 GGTACC GGTAC^C
MamI 4174 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2703 ACGCGT A^CGCGT
NaeI 774 GCCGGC GCC^GGC
NarI 1797 GGCGCC GG^CGCC
NgoMIV 774 GCCGGC G^CCGGC
NsiI 277 ATGCAT ATGCA^T
PaeR7I 568 CTCGAG C^TCGAG
PflFI 2349 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4480 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4321 ACCGGT A^CCGGT
PshAI 1961 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2099 AACGTT AA^CGTT
SexAI 298 ACCTGGT A^CC[AT]GGT
SfoI 1797 GGCGCC GGC^GCC
SspI 1027 AATATT AAT^ATT
StuI 2513 AGGCCT AGG^CCT
Tth111I 2349 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4480 CCACACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 568 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2481 GTAGAC GT^[AC][GT]AC
4389 GTATAC
AccII 1646 CGCG CG^CG
2704 CGCG
AseI 3032 ATTAAT AT^TAAT
3173 ATTAAT
AsnI 3032 ATTAAT AT^TAAT
3173 ATTAAT
AvrII 4011 CCTAGG C^CTAGG
4202 CCTAGG
BalI 3946 TGGCCA TGG^CCA
4488 TGGCCA
BamHI 1622 GGATCC G^GATCC
4799 GGATCC
BlnI 4011 CCTAGG C^CTAGG
4202 CCTAGG
BsaI 2954 GAGACC GGTCTC(1/5)
3849 GGTCTC
BsaWI 2015 ACCGGA [AT]^CCGG[AT]
4321 ACCGGT
BsrBI 1552 GAGCGG CCGCTC(-3/-3)
1890 GAGCGG
BsrDI 455 GCAATG GCAATG(2/0)
2005 GCAATG
BstAPI 270 GCAGCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4187 GCAGCCTCTGC
BstUI 1646 CGCG CG^CG
2704 CGCG
DrdI 1874 GACCCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2349 GACTCAGTCGTC
HaeII 1797 GGCGCC [GA]GCGC^[CT]
4499 AGCGCT
HindIII 2507 AAGCTT A^AGCTT
4643 AAGCTT
MluNI 3946 TGGCCA TGG^CCA
4488 TGGCCA
MscI 3946 TGGCCA TGG^CCA
4488 TGGCCA
MvnI 1646 CGCG CG^CG
2704 CGCG
NdeI 1632 CATATG CA^TATG
1640 CATATG
PshBI 3032 ATTAAT AT^TAAT
3173 ATTAAT
SacI 2918 GAGCTC GAGCT^C
3043 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII