##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AviII BbeI
BbrPI BcgI BclI BfrI BsaBI
BsiEI BsiWI BspDI BspLU11I BsrDI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MunI NarI
NdeI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SfoI SgrAI SnaBI SpeI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1600 GTCTAC GT^[AC][GT]AC
AcsI 4402 GAATTT [GA]^AATT[CT]
AflIII 3460 ACGCGT A^C[GA][CT]GT
AhdI 1204 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4402 GAATTT [GA]^AATT[CT]
AspEI 1204 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4045 CCTAGG C^CTAGG
BamHI 521 GGATCC G^GATCC
BciVI 2580 GTATCC GTATCC(6/5)
BglII 1322 AGATCT A^GATCT
BlnI 4045 CCTAGG C^CTAGG
BsmBI 574 GAGACG CGTCTC(1/5)
BsmI 2035 GAATGC GAATGC(1/-1)
Bsp1407I 389 TGTACA T^GTACA
BsrGI 389 TGTACA T^GTACA
BstAPI 2812 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 146 TTCGAA TT^CGAA
DrdI 1717 GACGAGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1204 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2904 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2733 GATATC GAT^ATC
HaeII 1496 AGCGCC [GA]GCGC^[CT]
MluI 3460 ACGCGT A^CGCGT
NaeI 1508 GCCGGC GCC^GGC
NgoMIV 1508 GCCGGC G^CCGGC
NspV 146 TTCGAA TT^CGAA
PaeR7I 796 CTCGAG C^TCGAG
SbfI 2596 CCTGCAGG CCTGCA^GG
ScaI 2000 AGTACT AGT^ACT
SfiI 1076 GGCCTGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 146 TTCGAA TT^CGAA
Sse8387I 2596 CCTGCAGG CCTGCA^GG
SspBI 389 TGTACA T^GTACA
SspI 4428 AATATT AAT^ATT
XhoI 796 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1881 TCCGGA T^CCGGA
2051 TCCGGA
AcyI 55 GACGCC G[GA]^CG[CT]C
2151 GACGCC
AocI 1258 CCTGAGG CC^T[ACGT]AGG
3226 CCTTAGG
Asp700I 1264 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3575 GAACACATTC
BbvCI 916 CCTCAGC CCTCAGC(-5/-2)
4170 CCTCAGC
BlpI 1247 GCTCAGC GC^T[ACGT]AGC
1761 GCTTAGC
Bpu1102I 1247 GCTCAGC GC^T[ACGT]AGC
1761 GCTTAGC
BsaAI 1348 TACGTG [CT]AC^GT[GA]
2172 CACGTA
BsaHI 55 GACGCC G[GA]^CG[CT]C
2151 GACGCC
BseAI 1881 TCCGGA T^CCGGA
2051 TCCGGA
BspEI 1881 TCCGGA T^CCGGA
2051 TCCGGA
BspHI 3583 TCATGA T^CATGA
4368 TCATGA
BsrBI 1738 GAGCGG CCGCTC(-3/-3)
2984 GAGCGG
BsrFI 1499 GCCGGT [GA]^CCGG[CT]
1508 GCCGGC
BssSI 343 CACGAG CACGAG(-5/-1)
2981 CACGAG
Bsu36I 1258 CCTGAGG CC^T[ACGT]AGG
3226 CCTTAGG
CelII 1247 GCTCAGC GC^T[ACGT]AGC
1761 GCTTAGC
Cfr10I 1499 GCCGGT [GA]^CCGG[CT]
1508 GCCGGC
Eco81I 1258 CCTGAGG CC^T[ACGT]AGG
3226 CCTTAGG
Hin1I 55 GACGCC G[GA]^CG[CT]C
2151 GACGCC
MaeII 1349 ACGT A^CGT
2173 ACGT
MroI 1881 TCCGGA T^CCGGA
2051 TCCGGA
NcoI 1595 CCATGG C^CATGG
4230 CCATGG
NheI 222 GCTAGC G^CTAGC
2631 GCTAGC
RcaI 3583 TCATGA T^CATGA
4368 TCATGA
SexAI 1703 ACCAGGT A^CC[AT]GGT
2873 ACCAGGT
SmlI 691 CTCAAG C^T[CT][GA]AG
796 CTCGAG
SphI 2133 GCATGC GCATG^C
3107 GCATGC
StuI 1917 AGGCCT AGG^CCT
1940 AGGCCT
Tsp45I 610 GTCAC ^GT[GC]AC
2543 GTGAC
XbaI 2805 TCTAGA T^CTAGA
4138 TCTAGA
XmnI 1264 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3575 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI