##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbeI BcgI BciVI BfrI
BglI BsaBI BsiEI BsiWI BspDI
BsrBI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI KasI
KspI MamI MluI MvnI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI PvuI RsrII SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 996 TCCGGA T^CCGGA
AclI 1580 AACGTT AA^CGTT
AcyI 2734 GACGCC G[GA]^CG[CT]C
AflIII 4518 ACATGT A^C[GA][CT]GT
Alw44I 4043 GTGCAC G^TGCAC
ApaI 4093 GGGCCC GGGCC^C
ApaLI 4043 GTGCAC G^TGCAC
AspI 70 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2654 CCTAGG C^CTAGG
BamHI 421 GGATCC G^GATCC
BbrPI 1054 CACGTG CAC^GTG
BglII 1314 AGATCT A^GATCT
BlnI 2654 CCTAGG C^CTAGG
BsaAI 1054 CACGTG [CT]AC^GT[GA]
BsaHI 2734 GACGCC G[GA]^CG[CT]C
BseAI 996 TCCGGA T^CCGGA
Bsp1407I 2563 TGTACA T^GTACA
BspEI 996 TCCGGA T^CCGGA
BspHI 750 TCATGA T^CATGA
BspLU11I 4518 ACATGT A^CATGT
BsrGI 2563 TGTACA T^GTACA
BssSI 584 CTCGTG CACGAG(-5/-1)
BstEII 406 GGTGACC G^GT[ACGT]ACC
BstPI 406 GGTGACC G^GT[ACGT]ACC
DrdI 337 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 406 GGTGACC G^GT[ACGT]ACC
HaeII 141 GGCGCT [GA]GCGC^[CT]
Hin1I 2734 GACGCC G[GA]^CG[CT]C
HindIII 2457 AAGCTT A^AGCTT
HpaI 1289 GTTAAC GTT^AAC
MroI 996 TCCGGA T^CCGGA
NaeI 2475 GCCGGC GCC^GGC
NdeI 1514 CATATG CA^TATG
NgoMIV 2475 GCCGGC G^CCGGC
PflFI 70 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1054 CACGTG CAC^GTG
PmlI 1054 CACGTG CAC^GTG
Psp1406I 1580 AACGTT AA^CGTT
RcaI 750 TCATGA T^CATGA
SacI 3230 GAGCTC GAGCT^C
SspBI 2563 TGTACA T^GTACA
Tth111I 70 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1361 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 635 GGTACC G^GTACC
3651 GGTACC
AccI 334 GTAGAC GT^[AC][GT]AC
4560 GTCTAC
Asp700I 837 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1988 GAAAAGTTTC
Asp718I 635 GGTACC G^GTACC
3651 GGTACC
BclI 595 TGATCA T^GATCA
4699 TGATCA
BlpI 454 GCTGAGC GC^T[ACGT]AGC
1031 GCTCAGC
Bpu1102I 454 GCTGAGC GC^T[ACGT]AGC
1031 GCTCAGC
BsmBI 460 CGTCTC CGTCTC(1/5)
3183 GAGACG
BsrDI 3500 CATTGC GCAATG(2/0)
1490 GCAATG
BsrFI 632 GCCGGT [GA]^CCGG[CT]
2475 GCCGGC
BstXI 2837 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3398 CCATCCAACTGG
CelII 454 GCTGAGC GC^T[ACGT]AGC
1031 GCTCAGC
CfoI 142 GCGC GCG^C
903 GCGC
Cfr10I 632 GCCGGT [GA]^CCGG[CT]
2475 GCCGGC
DraI 1019 TTTAAA TTT^AAA
4213 TTTAAA
DsaI 1331 CCGTGG C^C[GA][CT]GG
2463 CCACGG
FbaI 595 TGATCA T^GATCA
4699 TGATCA
HhaI 142 GCGC GCG^C
903 GCGC
HinP1I 142 GCGC G^CGC
903 GCGC
KpnI 635 GGTACC GGTAC^C
3651 GGTACC
MfeI 591 CAATTG C^AATTG
1928 CAATTG
MunI 591 CAATTG C^AATTG
1928 CAATTG
SbfI 295 CCTGCAGG CCTGCA^GG
1129 CCTGCAGG
SexAI 2989 ACCTGGT A^CC[AT]GGT
3647 ACCTGGT
SmaI 1092 CCCGGG CCC^GGG
3206 CCCGGG
Sse8387I 295 CCTGCAGG CCTGCA^GG
1129 CCTGCAGG
XcmI 212 CCAGTCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4079 CCACTGTTTTACTGG
XmaCI 1092 CCCGGG C^CCGGG
3206 CCCGGG
XmaI 1092 CCCGGG C^CCGGG
3206 CCCGGG
XmnI 837 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1988 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII