##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI AviII BbrPI BfrI BsaBI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2713 GACGTC GACGT^C
AccII 60 CGCG CG^CG
AcsI 3696 GAATTT [GA]^AATT[CT]
Alw44I 1961 GTGCAC G^TGCAC
ApaLI 1961 GTGCAC G^TGCAC
ApoI 3696 GAATTT [GA]^AATT[CT]
AseI 4176 ATTAAT AT^TAAT
AsnI 4176 ATTAAT AT^TAAT
BbeI 611 GGCGCC GGCGC^C
BcgI 3295 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2292 GTATCC GTATCC(6/5)
BclI 1253 TGATCA T^GATCA
BsaWI 87 TCCGGT [AT]^CCGG[AT]
BsmI 4088 GAATGC GAATGC(1/-1)
BspLU11I 3033 ACATGT A^CATGT
BsrBI 111 CCGCTC CCGCTC(-3/-3)
BssSI 2875 CTCGTG CACGAG(-5/-1)
BstUI 60 CGCG CG^CG
DraI 3076 TTTAAA TTT^AAA
DraIII 2697 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 1253 TGATCA T^GATCA
HincII 3527 GTTGAC GT[CT]^[GA]AC
HindII 3527 GTTGAC GT[CT]^[GA]AC
KasI 611 GGCGCC G^GCGCC
KspI 59 CCGCGG CCGC^GG
MvnI 60 CGCG CG^CG
NarI 611 GGCGCC GG^CGCC
NdeI 1492 CATATG CA^TATG
NheI 679 GCTAGC G^CTAGC
PaeR7I 3293 CTCGAG C^TCGAG
PshBI 4176 ATTAAT AT^TAAT
SacII 59 CCGCGG CCGC^GG
SfiI 4359 GGCCCCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 611 GGCGCC GGC^GCC
SmaI 33 CCCGGG CCC^GGG
XhoI 3293 CTCGAG C^TCGAG
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1623 ACGTGT A^C[GA][CT]GT
3033 ACATGT
AocI 150 CCTGAGG CC^T[ACGT]AGG
1516 CCTGAGG
Asp700I 170 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
383 GAACACCTTC
AvrII 2024 CCTAGG C^CTAGG
3534 CCTAGG
BamHI 451 GGATCC G^GATCC
1364 GGATCC
BbvCI 2983 CCTCAGC CCTCAGC(-5/-2)
3744 CCTCAGC
BlnI 2024 CCTAGG C^CTAGG
3534 CCTAGG
BlpI 1766 GCTGAGC GC^T[ACGT]AGC
1795 GCTGAGC
Bpu1102I 1766 GCTGAGC GC^T[ACGT]AGC
1795 GCTGAGC
BsrDI 2013 CATTGC GCAATG(2/0)
4344 GCAATG
Bsu36I 150 CCTGAGG CC^T[ACGT]AGG
1516 CCTGAGG
CelII 1766 GCTGAGC GC^T[ACGT]AGC
1795 GCTGAGC
Eco81I 150 CCTGAGG CC^T[ACGT]AGG
1516 CCTGAGG
EcoNI 602 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1708 CCTCCCAAAGG
HgaI 334 GCGTC GACGC(5/10)
2666 GCGTC
NcoI 1984 CCATGG C^CATGG
3488 CCATGG
PflMI 2582 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4321 CCAGGGTATGG
SacI 1900 GAGCTC GAGCT^C
2347 GAGCTC
SapI 1108 GAAGAGC GCTCTTC(1/4)
3151 GCTCTTC
Van91I 2582 CCAATGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4321 CCAGGGTATGG
XcmI 1778 CCAGGAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2759 CCAAAGGCTGCGTGG
XmnI 170 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
383 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII