##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AviII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SfoI SfuI SgrAI
SnaBI SpeI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 751 TCCGGA T^CCGGA
AcsI 404 GAATTT [GA]^AATT[CT]
AhdI 3934 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 404 GAATTT [GA]^AATT[CT]
Asp700I 529 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3934 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4097 CCTAGG C^CTAGG
BamHI 4101 GGATCC G^GATCC
BlnI 4097 CCTAGG C^CTAGG
BlpI 2970 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2970 GCTCAGC GC^T[ACGT]AGC
BsaAI 1280 CACGTA [CT]AC^GT[GA]
BsaWI 751 TCCGGA [AT]^CCGG[AT]
BseAI 751 TCCGGA T^CCGGA
BsiEI 651 CGGCCG CG[GA][CT]^CG
BspEI 751 TCCGGA T^CCGGA
BssSI 3001 CACGAG CACGAG(-5/-1)
BstYI 4101 GGATCC [GA]^GATC[CT]
CelII 2970 GCTCAGC GC^T[ACGT]AGC
DrdI 7 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 651 CGGCCG C^GGCCG
Eam1105I 3934 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 651 CGGCCG C^GGCCG
Eco52I 651 CGGCCG C^GGCCG
KspI 2458 CCGCGG CCGC^GG
MflI 4101 GGATCC [GA]^GATC[CT]
MroI 751 TCCGGA T^CCGGA
PaeR7I 696 CTCGAG C^TCGAG
SacII 2458 CCGCGG CCGC^GG
SbfI 3188 CCTGCAGG CCTGCA^GG
SexAI 1636 ACCAGGT A^CC[AT]GGT
SphI 729 GCATGC GCATG^C
Sse8387I 3188 CCTGCAGG CCTGCA^GG
SspI 4308 AATATT AAT^ATT
Tsp509I 405 AATT ^AATT
XhoI 696 CTCGAG C^TCGAG
XhoII 4101 GGATCC [GA]^GATC[CT]
XmnI 529 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 13 GACGTC GACGT^C
870 GACGTC
AflIII 1671 ACATGT A^C[GA][CT]GT
3980 ACATGT
AspI 887 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2741 GACAGGGTC
BsmBI 872 CGTCTC CGTCTC(1/5)
1662 GAGACG
BspLU11I 1671 ACATGT A^CATGT
3980 ACATGT
BsrDI 1275 CATTGC GCAATG(2/0)
2814 GCAATG
BstAPI 322 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4153 GCACGGTCTGC
BstEII 2746 GGTCACC G^GT[ACGT]ACC
3613 GGTCACC
BstPI 2746 GGTCACC G^GT[ACGT]ACC
3613 GGTCACC
DraI 3779 TTTAAA TTT^AAA
4268 TTTAAA
EcoO65I 2746 GGTCACC G^GT[ACGT]ACC
3613 GGTCACC
HaeII 1800 AGCGCC [GA]GCGC^[CT]
3100 AGCGCC
MseI 3780 TTAA T^TAA
4269 TTAA
NaeI 986 GCCGGC GCC^GGC
2841 GCCGGC
NcoI 1384 CCATGG C^CATGG
4055 CCATGG
NgoMIV 986 GCCGGC G^CCGGC
2841 GCCGGC
PflFI 887 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2741 GACAGGGTC
PshAI 592 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3979 GACATGTGTC
PvuII 309 CAGCTG CAG^CTG
3213 CAGCTG
SacI 1297 GAGCTC GAGCT^C
3299 GAGCTC
SmlI 696 CTCGAG C^T[CT][GA]AG
706 CTTGAG
TaqI 697 TCGA T^CGA
3279 TCGA
Tru9I 3780 TTAA T^TAA
4269 TTAA
Tth111I 887 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2741 GACAGGGTC
TthHB8I 697 TCGA T^CGA
3279 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
StuI StyI TfiI TseI Tsp45I
TspRI Van91I XcmI XmaCI XmaI