##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflII Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AvrII
BclI BfrI BglII BlnI BsiEI
BsiWI BssSI Bst1107I BstBI BstZ17I
DraI DraIII EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HpaI MfeI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshBI Psp1406I
PvuI SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2215 GGTACC G^GTACC
AccI 963 GTCTAC GT^[AC][GT]AC
AcsI 3419 AAATTC [GA]^AATT[CT]
AgeI 709 ACCGGT A^CCGGT
AhdI 1371 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3419 AAATTC [GA]^AATT[CT]
Asp718I 2215 GGTACC G^GTACC
AspEI 1371 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1308 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1249 TGCGCA TGC^GCA
BamHI 4413 GGATCC G^GATCC
BbeI 1 GGCGCC GGCGC^C
BcgI 3017 GCAGGGAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2094 GTATCC GTATCC(6/5)
BsaBI 881 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 228 GCATTC GAATGC(1/-1)
BspDI 3635 ATCGAT AT^CGAT
BssHII 1149 GCGCGC G^CGCGC
ClaI 3635 ATCGAT AT^CGAT
Eam1105I 1371 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1249 TGCGCA TGC^GCA
HincII 555 GTCAAC GT[CT]^[GA]AC
HindII 555 GTCAAC GT[CT]^[GA]AC
HindIII 3896 AAGCTT A^AGCTT
KasI 1 GGCGCC G^GCGCC
KpnI 2215 GGTACC GGTAC^C
KspI 1109 CCGCGG CCGC^GG
MamI 881 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1844 ACGCGT A^CGCGT
NaeI 4 GCCGGC GCC^GGC
NarI 1 GGCGCC GG^CGCC
NgoMIV 4 GCCGGC G^CCGGC
PflFI 1308 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 709 ACCGGT A^CCGGT
PshAI 2504 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2263 GAGCTC GAGCT^C
SacII 1109 CCGCGG CCGC^GG
SapI 3837 GAAGAGC GCTCTTC(1/4)
SexAI 1822 ACCAGGT A^CC[AT]GGT
SfiI 2175 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1 GGCGCC GGC^GCC
SmlI 366 CTCAAG C^T[CT][GA]AG
Tth111I 1308 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 798 GACGTC GACGT^C
1158 GACGTC
AccIII 940 TCCGGA T^CCGGA
2267 TCCGGA
BalI 3517 TGGCCA TGG^CCA
4607 TGGCCA
BbrPI 3156 CACGTG CAC^GTG
3731 CACGTG
BseAI 940 TCCGGA T^CCGGA
2267 TCCGGA
Bsp1407I 178 TGTACA T^GTACA
1132 TGTACA
BspEI 940 TCCGGA T^CCGGA
2267 TCCGGA
BspHI 283 TCATGA T^CATGA
862 TCATGA
BspLU11I 181 ACATGT A^CATGT
1129 ACATGT
BsrBI 4273 CCGCTC CCGCTC(-3/-3)
435 GAGCGG
BsrGI 178 TGTACA T^GTACA
1132 TGTACA
CpoI 15 CGGACCG CG^G[AT]CCG
779 CGGACCG
DrdI 1620 GACAGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2766 GACCTGGGGGTC
MluNI 3517 TGGCCA TGG^CCA
4607 TGGCCA
MroI 940 TCCGGA T^CCGGA
2267 TCCGGA
MscI 3517 TGGCCA TGG^CCA
4607 TGGCCA
NspI 181 ACATGT [GA]CATG^[CT]
1129 ACATGT
PflMI 1367 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3404 CCAAATCTTGG
PmaCI 3156 CACGTG CAC^GTG
3731 CACGTG
PmlI 3156 CACGTG CAC^GTG
3731 CACGTG
RcaI 283 TCATGA T^CATGA
862 TCATGA
RsrII 15 CGGACCG CG^G[AT]CCG
779 CGGACCG
SspBI 178 TGTACA T^GTACA
1132 TGTACA
TaqI 2723 TCGA T^CGA
3636 TCGA
TthHB8I 2723 TCGA T^CGA
3636 TCGA
Van91I 1367 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3404 CCAAATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmaCI XmaI