##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
AspI AviII BamHI BciVI BfrI
BseAI BsiWI BsmBI BspEI BspHI
BsrDI BssHII Bst1107I BstBI BstZ17I
CpoI DraI DrdI EcoRI EcoRV
FseI FspI HindIII HpaI KspI
MfeI MroI MunI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I RcaI RsrII
SacII SalI SapI SbfI ScaI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tsp509I Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 917 GACGTC GACGT^C
Acc65I 2922 GGTACC G^GTACC
AhdI 2303 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1559 GTGCAC G^TGCAC
AocI 4310 CCTGAGG CC^T[ACGT]AGG
ApaLI 1559 GTGCAC G^TGCAC
Asp718I 2922 GGTACC G^GTACC
AspEI 2303 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3228 CCTAGG C^CTAGG
BbsI 1625 GTCTTC GAAGAC(2/6)
BclI 936 TGATCA T^GATCA
BlnI 3228 CCTAGG C^CTAGG
BpuAI 1625 GTCTTC GAAGAC(2/6)
BsaWI 796 ACCGGA [AT]^CCGG[AT]
BsmI 240 GCATTC GAATGC(1/-1)
BspDI 1002 ATCGAT AT^CGAT
BspLU11I 2178 ACATGT A^CATGT
BssSI 1958 CACGAG CACGAG(-5/-1)
Bsu36I 4310 CCTGAGG CC^T[ACGT]AGG
ClaI 1002 ATCGAT AT^CGAT
DraIII 1489 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 494 CGGCCG C^GGCCG
Eam1105I 2303 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 494 CGGCCG C^GGCCG
Eco52I 494 CGGCCG C^GGCCG
Eco81I 4310 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1070 ATGCAT ATGCA^T
FbaI 936 TGATCA T^GATCA
HincII 2174 GTTGAC GT[CT]^[GA]AC
HindII 2174 GTTGAC GT[CT]^[GA]AC
KpnI 2922 GGTACC GGTAC^C
MluI 4 ACGCGT A^CGCGT
NaeI 4249 GCCGGC GCC^GGC
NdeI 162 CATATG CA^TATG
NgoMIV 4249 GCCGGC G^CCGGC
NheI 2419 GCTAGC G^CTAGC
NsiI 1070 ATGCAT ATGCA^T
PshAI 913 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2431 CGATCG CGAT^CG
SexAI 4018 ACCTGGT A^CC[AT]GGT
SfiI 3812 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3784 CCCGGG CCC^GGG
XbaI 3304 TCTAGA T^CTAGA
XmaCI 3784 CCCGGG C^CCGGG
XmaI 3784 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
ApaI 3755 GGGCCC GGGCC^C
3820 GGGCCC
BbeI 3160 GGCGCC GGCGC^C
3761 GGCGCC
BbvCI 3188 CCTCAGC CCTCAGC(-5/-2)
3195 CCTCAGC
BcgI 946 GCAGAAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2827 GCAAGGCCCTCG
BsiEI 494 CGGCCG CG[GA][CT]^CG
2431 CGATCG
BsrFI 1563 ACCGGC [GA]^CCGG[CT]
4249 GCCGGC
Cfr10I 1563 ACCGGC [GA]^CCGG[CT]
4249 GCCGGC
HgaI 3 GACGC GACGC(5/10)
4344 GACGC
KasI 3160 GGCGCC G^GCGCC
3761 GGCGCC
NarI 3160 GGCGCC GG^CGCC
3761 GGCGCC
PpuMI 3685 GGGACCC [GA]G^G[AT]CC[CT]
4183 AGGACCC
SacI 1832 GAGCTC GAGCT^C
3747 GAGCTC
SfoI 3160 GGCGCC GGC^GCC
3761 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmnI