##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI Aor51HI ApaI AscI
Asp718I AvrII BbeI BbrPI BbvCI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaHI BsiWI BspDI BspLU11I
BsrDI BssHII BssSI Bst1107I BstAPI
BstZ17I CelII ClaI CpoI DraI
DraII DrdI EagI EclXI Eco47III
Eco52I EcoO109I EcoRV FseI HaeII
Hin1I KasI KpnI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
RsrII SalI SbfI SexAI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AhdI 2366 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2240 GTGCAC G^TGCAC
AlwNI 4252 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2240 GTGCAC G^TGCAC
AseI 4165 ATTAAT AT^TAAT
AsnI 4165 ATTAAT AT^TAAT
AspEI 2366 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2958 CTCGGG C^[CT]CG[GA]G
AviII 2396 TGCGCA TGC^GCA
BanI 1001 GGTGCC G^G[CT][GA]CC
BglI 2424 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 233 CACGTA [CT]AC^GT[GA]
BsaBI 385 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2356 GGTCTC GGTCTC(1/5)
BsmBI 3341 CGTCTC CGTCTC(1/5)
BsmI 586 GAATGC GAATGC(1/-1)
BsoBI 2958 CTCGGG C^[CT]CG[GA]G
BsrBI 3428 GAGCGG CCGCTC(-3/-3)
BsrFI 998 GCCGGT [GA]^CCGG[CT]
BstBI 1733 TTCGAA TT^CGAA
BstEII 322 GGTCACC G^GT[ACGT]ACC
BstPI 322 GGTCACC G^GT[ACGT]ACC
Cfr10I 998 GCCGGT [GA]^CCGG[CT]
Eam1105I 2366 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 322 GGTCACC G^GT[ACGT]ACC
EcoT22I 4176 ATGCAT ATGCA^T
FauI 3747 GCGGG CCCGC(4/6)
FspI 2396 TGCGCA TGC^GCA
HincII 2591 GTTAAC GT[CT]^[GA]AC
HindII 2591 GTTAAC GT[CT]^[GA]AC
HpaI 2591 GTTAAC GTT^AAC
KspI 2448 CCGCGG CCGC^GG
MaeII 234 ACGT A^CGT
MamI 385 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3692 CCATGG C^CATGG
NdeI 4418 CATATG CA^TATG
NsiI 4176 ATGCAT ATGCA^T
NspV 1733 TTCGAA TT^CGAA
PshAI 1760 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4165 ATTAAT AT^TAAT
PvuI 28 CGATCG CGAT^CG
PvuII 476 CAGCTG CAG^CTG
SacII 2448 CCGCGG CCGC^GG
ScaI 2406 AGTACT AGT^ACT
SfiI 2423 GGCCTGGTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1733 TTCGAA TT^CGAA
SphI 2274 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2495 GTCTAC GT^[AC][GT]AC
2681 GTCTAC
AccII 1111 CGCG CG^CG
2449 CGCG
AccIII 2538 TCCGGA T^CCGGA
2913 TCCGGA
AocI 3412 CCTCAGG CC^T[ACGT]AGG
3919 CCTGAGG
AspI 1283 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2879 GACAGGGTC
BalI 1026 TGGCCA TGG^CCA
3846 TGGCCA
BseAI 2538 TCCGGA T^CCGGA
2913 TCCGGA
BsgI 1778 CTGCAC GTGCAG(16/14)
2757 GTGCAG
BsiEI 28 CGATCG CG[GA][CT]^CG
1402 CGACCG
BspEI 2538 TCCGGA T^CCGGA
2913 TCCGGA
BstUI 1111 CGCG CG^CG
2449 CGCG
Bsu36I 3412 CCTCAGG CC^T[ACGT]AGG
3919 CCTGAGG
DraIII 843 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1695 CACTCCGTG
EaeI 1026 TGGCCA [CT]^GGCC[GA]
3846 TGGCCA
Eco81I 3412 CCTCAGG CC^T[ACGT]AGG
3919 CCTGAGG
EcoNI 2437 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3585 CCTGGAGAAGG
EcoT14I 98 CCAAGG C^C[AT][AT]GG
3692 CCATGG
HgaI 3042 GCGTC GACGC(5/10)
3340 GCGTC
HindIII 2705 AAGCTT A^AGCTT
3672 AAGCTT
MluNI 1026 TGGCCA TGG^CCA
3846 TGGCCA
MroI 2538 TCCGGA T^CCGGA
2913 TCCGGA
MscI 1026 TGGCCA TGG^CCA
3846 TGGCCA
MvnI 1111 CGCG CG^CG
2449 CGCG
PflFI 1283 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2879 GACAGGGTC
PflMI 1012 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2179 CCAGAACATGG
SacI 1585 GAGCTC GAGCT^C
2453 GAGCTC
SmlI 637 CTCAAG C^T[CT][GA]AG
1963 CTTGAG
SspI 2324 AATATT AAT^ATT
3457 AATATT
StyI 98 CCAAGG C^C[AT][AT]GG
3692 CCATGG
Tth111I 1283 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2879 GACAGGGTC
Van91I 1012 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2179 CCAGAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BamHI
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRI EcoRII FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI