##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Aor51HI AseI AsnI Asp718I AvrII
BamHI BbeI BbrPI BcgI BfrI
BglII BlnI BseAI BsiWI BspDI
BspEI BspLU11I BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eco47III
EcoRV FseI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Van91I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 3585 AACGTT AA^CGTT
AcyI 3774 GGCGTC G[GA]^CG[CT]C
AhdI 1590 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 52 GGCGCGCC GG^CGCGCC
AspEI 1590 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2144 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 398 TGCGCA TGC^GCA
BbsI 2015 GAAGAC GAAGAC(2/6)
BbvCI 2028 CCTCAGC CCTCAGC(-5/-2)
BciVI 3363 GGATAC GTATCC(6/5)
BclI 3563 TGATCA T^GATCA
BlpI 306 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
BpuAI 2015 GAAGAC GAAGAC(2/6)
BsaBI 102 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3774 GGCGTC G[GA]^CG[CT]C
BsaI 3434 GAGACC GGTCTC(1/5)
BsaWI 3217 TCCGGT [AT]^CCGG[AT]
BsgI 589 CTGCAC GTGCAG(16/14)
BsiEI 59 CGGCCG CG[GA][CT]^CG
BsmBI 2871 CGTCTC CGTCTC(1/5)
Bsp1407I 1127 TGTACA T^GTACA
BspHI 2109 TCATGA T^CATGA
BsrGI 1127 TGTACA T^GTACA
BssHII 53 GCGCGC G^CGCGC
BstXI 1567 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 306 GCTCAGC GC^T[ACGT]AGC
DraI 2221 TTTAAA TTT^AAA
EagI 59 CGGCCG C^GGCCG
Eam1105I 1590 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 59 CGGCCG C^GGCCG
Eco52I 59 CGGCCG C^GGCCG
EcoRI 2558 GAATTC G^AATTC
EcoT22I 2963 ATGCAT ATGCA^T
FbaI 3563 TGATCA T^GATCA
FspI 398 TGCGCA TGC^GCA
Hin1I 3774 GGCGTC G[GA]^CG[CT]C
HindIII 1707 AAGCTT A^AGCTT
MamI 102 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 57 GCCGGC GCC^GGC
NcoI 2496 CCATGG C^CATGG
NgoMIV 57 GCCGGC G^CCGGC
NsiI 2963 ATGCAT ATGCA^T
PaeR7I 3936 CTCGAG C^TCGAG
PflFI 2144 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3585 AACGTT AA^CGTT
RcaI 2109 TCATGA T^CATGA
SfiI 1916 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1723 GCATGC GCATG^C
SspBI 1127 TGTACA T^GTACA
StuI 3498 AGGCCT AGG^CCT
TfiI 3147 GATTC G^A[AT]TC
Tth111I 2144 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3936 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2741 GTAGAC GT^[AC][GT]AC
3712 GTAGAC
AcsI 418 AAATTC [GA]^AATT[CT]
2558 GAATTC
ApoI 418 AAATTC [GA]^AATT[CT]
2558 GAATTC
Asp700I 723 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2268 GAAGTTTTTC
BsaAI 622 TACGTG [CT]AC^GT[GA]
752 TACGTG
BsmI 3681 GAATGC GAATGC(1/-1)
3660 GCATTC
BsrFI 57 GCCGGC [GA]^CCGG[CT]
1447 GCCGGT
BstAPI 1723 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3543 GCAAGCTCTGC
BstEII 1587 GGTGACC G^GT[ACGT]ACC
3252 GGTGACC
BstPI 1587 GGTGACC G^GT[ACGT]ACC
3252 GGTGACC
BstYI 107 AGATCC [GA]^GATC[CT]
3394 AGATCC
Cfr10I 57 GCCGGC [GA]^CCGG[CT]
1447 GCCGGT
DraIII 325 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2717 CACCAGGTG
EcoNI 3462 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3518 CCTCAAGGAGG
EcoO65I 1587 GGTGACC G^GT[ACGT]ACC
3252 GGTGACC
MflI 107 AGATCC [GA]^GATC[CT]
3394 AGATCC
NspI 1723 GCATGC [GA]CATG^[CT]
2961 ACATGC
SexAI 839 ACCAGGT A^CC[AT]GGT
2718 ACCAGGT
SmlI 3519 CTCAAG C^T[CT][GA]AG
3936 CTCGAG
XbaI 1267 TCTAGA T^CTAGA
3154 TCTAGA
XcmI 1759 CCATCGTGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3452 CCAGCGGCTGCCTGG
XhoII 107 AGATCC [GA]^GATC[CT]
3394 AGATCC
XmnI 723 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2268 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BssSI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI