##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI AlwI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BclI BlnI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MroI NarI
NdeI NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AflII 3506 CTTAAG C^TTAAG
AflIII 4 ACGCGT A^C[GA][CT]GT
Alw44I 1163 GTGCAC G^TGCAC
ApaLI 1163 GTGCAC G^TGCAC
Asp700I 2433 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4663 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2353 GGATAC GTATCC(6/5)
BfrI 3506 CTTAAG C^TTAAG
BglII 3170 AGATCT A^GATCT
BsaBI 1908 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2636 ACCGGA [AT]^CCGG[AT]
BssSI 1775 CTCGTG CACGAG(-5/-1)
BstXI 439 CCAGAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3170 AGATCT [GA]^GATC[CT]
DrdI 3847 GACTCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1701 ATGCAT ATGCA^T
MamI 1908 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4449 CAATTG C^AATTG
MflI 3170 AGATCT [GA]^GATC[CT]
MluI 4 ACGCGT A^CGCGT
MunI 4449 CAATTG C^AATTG
NsiI 1701 ATGCAT ATGCA^T
PflFI 4663 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2788 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 3143 GCTCTTC GCTCTTC(1/4)
SbfI 1081 CCTGCAGG CCTGCA^GG
SphI 1699 GCATGC GCATG^C
Sse8387I 1081 CCTGCAGG CCTGCA^GG
Tsp509I 4450 AATT ^AATT
Tth111I 4663 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2788 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3170 AGATCT [GA]^GATC[CT]
XmnI 2433 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 3242 GACGTC GACGT^C
4360 GACGTC
AhdI 1872 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4355 GACAGGACGTC
AspEI 1872 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4355 GACAGGACGTC
BcgI 4444 CGAGTCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2527 GCACCTCCTTCG
BlpI 1587 GCTCAGC GC^T[ACGT]AGC
1853 GCTGAGC
Bpu1102I 1587 GCTCAGC GC^T[ACGT]AGC
1853 GCTGAGC
BsaI 1046 GAGACC GGTCTC(1/5)
2223 GGTCTC
BsrDI 2590 GCAATG GCAATG(2/0)
4421 GCAATG
CelII 1587 GCTCAGC GC^T[ACGT]AGC
1853 GCTGAGC
Eam1105I 1872 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4355 GACAGGACGTC
EarI 492 CTCTTC CTCTTC(1/4)
3144 CTCTTC
HindIII 3038 AAGCTT A^AGCTT
3617 AAGCTT
Ksp632I 492 CTCTTC CTCTTC(1/4)
3144 CTCTTC
NaeI 886 GCCGGC GCC^GGC
1140 GCCGGC
NcoI 307 CCATGG C^CATGG
3772 CCATGG
NgoMIV 886 GCCGGC G^CCGGC
1140 GCCGGC
PaeR7I 2081 CTCGAG C^TCGAG
3958 CTCGAG
SmaI 970 CCCGGG CCC^GGG
2421 CCCGGG
XhoI 2081 CTCGAG C^TCGAG
3958 CTCGAG
XmaCI 970 CCCGGG C^CCGGG
2421 CCCGGG
XmaI 970 CCCGGG C^CCGGG
2421 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I