##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BciVI BglI
BlnI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI DrdI EagI
EclXI Eco52I EcoRI FseI FspI
Hin1I HindIII KasI KpnI KspI
MamI MluI MroI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 517 CGCG CG^CG
AclI 3796 AACGTT AA^CGTT
AflII 2911 CTTAAG C^TTAAG
AflIII 2363 ACATGT A^C[GA][CT]GT
AhdI 1298 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 924 CCTCAGG CC^T[ACGT]AGG
Aor51HI 436 AGCGCT AGC^GCT
AspEI 1298 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 758 CCTCAGC CCTCAGC(-5/-2)
BfrI 2911 CTTAAG C^TTAAG
BglII 3886 AGATCT A^GATCT
BsgI 2332 GTGCAG GTGCAG(16/14)
BsmI 958 GCATTC GAATGC(1/-1)
BspHI 2048 TCATGA T^CATGA
BspLU11I 2363 ACATGT A^CATGT
BssSI 494 CACGAG CACGAG(-5/-1)
BstEII 478 GGTGACC G^GT[ACGT]ACC
BstPI 478 GGTGACC G^GT[ACGT]ACC
BstUI 517 CGCG CG^CG
Bsu36I 924 CCTCAGG CC^T[ACGT]AGG
CpoI 402 CGGACCG CG^G[AT]CCG
DraIII 4002 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1298 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 436 AGCGCT AGC^GCT
Eco81I 924 CCTCAGG CC^T[ACGT]AGG
EcoNI 920 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 478 GGTGACC G^GT[ACGT]ACC
EcoRV 3237 GATATC GAT^ATC
EcoT22I 379 ATGCAT ATGCA^T
HpaI 3905 GTTAAC GTT^AAC
MfeI 1517 CAATTG C^AATTG
MunI 1517 CAATTG C^AATTG
MvnI 517 CGCG CG^CG
NaeI 982 GCCGGC GCC^GGC
NcoI 2354 CCATGG C^CATGG
NgoMIV 982 GCCGGC G^CCGGC
NsiI 379 ATGCAT ATGCA^T
NspI 2363 ACATGT [GA]CATG^[CT]
PaeR7I 814 CTCGAG C^TCGAG
Psp1406I 3796 AACGTT AA^CGTT
RcaI 2048 TCATGA T^CATGA
RsrII 402 CGGACCG CG^G[AT]CCG
SacI 1575 GAGCTC GAGCT^C
SapI 2873 GCTCTTC GCTCTTC(1/4)
SgrAI 544 CACCGGCG C[GA]^CCGG[CT]G
SmaI 636 CCCGGG CCC^GGG
XbaI 1309 TCTAGA T^CTAGA
XcmI 288 CCAATGGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 814 CTCGAG C^TCGAG
XmaCI 636 CCCGGG C^CCGGG
XmaI 636 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 284 GGGCCC GGGCC^C
1161 GGGCCC
BalI 300 TGGCCA TGG^CCA
2351 TGGCCA
BamHI 839 GGATCC G^GATCC
2287 GGATCC
BbsI 461 GAAGAC GAAGAC(2/6)
3717 GAAGAC
BcgI 408 GCATCTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
806 GCAGCAGGCTCG
BpuAI 461 GAAGAC GAAGAC(2/6)
3717 GAAGAC
BsaWI 1209 ACCGGA [AT]^CCGG[AT]
1747 TCCGGT
BsrBI 497 GAGCGG CCGCTC(-3/-3)
3465 GAGCGG
BsrDI 1220 CATTGC GCAATG(2/0)
2089 GCAATG
BstXI 360 CCATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2665 CCAAGTTTCTGG
EarI 2874 CTCTTC CTCTTC(1/4)
943 GAAGAG
HaeII 436 AGCGCT [GA]GCGC^[CT]
2524 AGCGCC
HgaI 627 GCGTC GACGC(5/10)
660 GCGTC
Ksp632I 2874 CTCTTC CTCTTC(1/4)
943 GAAGAG
MluNI 300 TGGCCA TGG^CCA
2351 TGGCCA
MscI 300 TGGCCA TGG^CCA
2351 TGGCCA
PflMI 336 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3376 CCAGCATTTGG
SexAI 1024 ACCTGGT A^CC[AT]GGT
2825 ACCTGGT
Van91I 336 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3376 CCAGCATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII