##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AgeI AscI AseI
AsnI Asp700I Asp718I AspI BbeI
BciVI BclI BglII BsaBI BsiEI
BsiWI BspDI BsrBI BssHII BstBI
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRI EcoRV FbaI FseI
HpaI KasI KpnI KspI MamI
MfeI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI SapI ScaI
SexAI SfoI SfuI SgrAI SpeI
SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 4332 GTATAC GT^[AC][GT]AC
AccIII 49 TCCGGA T^CCGGA
AclI 1886 AACGTT AA^CGTT
AflII 3932 CTTAAG C^TTAAG
AhdI 471 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 37 AGCGCT AGC^GCT
AspEI 471 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2376 TGCGCA TGC^GCA
BamHI 226 GGATCC G^GATCC
BbrPI 2394 CACGTG CAC^GTG
BcgI 1497 CGATCCACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3932 CTTAAG C^TTAAG
BlpI 1034 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1034 GCTCAGC GC^T[ACGT]AGC
BsaWI 49 TCCGGA [AT]^CCGG[AT]
BseAI 49 TCCGGA T^CCGGA
BsmBI 67 GAGACG CGTCTC(1/5)
BspEI 49 TCCGGA T^CCGGA
BsrFI 2086 GCCGGT [GA]^CCGG[CT]
BssSI 3797 CTCGTG CACGAG(-5/-1)
Bst1107I 4332 GTATAC GTA^TAC
BstZ17I 4332 GTATAC GTA^TAC
CelII 1034 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2086 GCCGGT [GA]^CCGG[CT]
Eam1105I 471 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 37 AGCGCT AGC^GCT
EcoT22I 4081 ATGCAT ATGCA^T
FauI 769 GCGGG CCCGC(4/6)
FspI 2376 TGCGCA TGC^GCA
HincII 2402 GTTGAC GT[CT]^[GA]AC
HindII 2402 GTTGAC GT[CT]^[GA]AC
MluI 2385 ACGCGT A^CGCGT
MroI 49 TCCGGA T^CCGGA
NdeI 4078 CATATG CA^TATG
NsiI 4081 ATGCAT ATGCA^T
PacI 906 TTAATTAA TTAAT^TAA
PaeR7I 951 CTCGAG C^TCGAG
PmaCI 2394 CACGTG CAC^GTG
PmlI 2394 CACGTG CAC^GTG
Psp1406I 1886 AACGTT AA^CGTT
SfiI 3433 GGCCTCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 4007 TACGTA TAC^GTA
TaqI 952 TCGA T^CGA
TthHB8I 952 TCGA T^CGA
XhoI 951 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 87 GACGCC G[GA]^CG[CT]C
2734 GGCGTC
AvrII 870 CCTAGG C^CTAGG
2144 CCTAGG
BbsI 488 GAAGAC GAAGAC(2/6)
1204 GAAGAC
BlnI 870 CCTAGG C^CTAGG
2144 CCTAGG
BpuAI 488 GAAGAC GAAGAC(2/6)
1204 GAAGAC
BsaHI 87 GACGCC G[GA]^CG[CT]C
2734 GGCGTC
BsgI 518 CTGCAC GTGCAG(16/14)
3392 CTGCAC
Bsp1407I 4090 TGTACA T^GTACA
4102 TGTACA
BspHI 1219 TCATGA T^CATGA
4273 TCATGA
BspLU11I 820 ACATGT A^CATGT
4296 ACATGT
BsrDI 4254 CATTGC GCAATG(2/0)
1304 GCAATG
BsrGI 4090 TGTACA T^GTACA
4102 TGTACA
BstEII 503 GGTCACC G^GT[ACGT]ACC
3575 GGTGACC
BstPI 503 GGTCACC G^GT[ACGT]ACC
3575 GGTGACC
DraIII 692 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4236 CACGGAGTG
EarI 81 GAAGAG CTCTTC(1/4)
4244 GAAGAG
EcoNI 2144 CCTAGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2169 CCTAACCAAGG
EcoO65I 503 GGTCACC G^GT[ACGT]ACC
3575 GGTGACC
Hin1I 87 GACGCC G[GA]^CG[CT]C
2734 GGCGTC
HindIII 2000 AAGCTT A^AGCTT
2108 AAGCTT
Ksp632I 81 GAAGAG CTCTTC(1/4)
4244 GAAGAG
PflMI 1086 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2555 CCAGATCCTGG
RcaI 1219 TCATGA T^CATGA
4273 TCATGA
SacI 651 GAGCTC GAGCT^C
1488 GAGCTC
SbfI 2542 CCTGCAGG CCTGCA^GG
3419 CCTGCAGG
SmaI 312 CCCGGG CCC^GGG
3567 CCCGGG
SphI 2379 GCATGC GCATG^C
2426 GCATGC
Sse8387I 2542 CCTGCAGG CCTGCA^GG
3419 CCTGCAGG
SspBI 4090 TGTACA T^GTACA
4102 TGTACA
StuI 3003 AGGCCT AGG^CCT
3289 AGGCCT
Van91I 1086 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2555 CCAGATCCTGG
XmaCI 312 CCCGGG C^CCGGG
3567 CCCGGG
XmaI 312 CCCGGG C^CCGGG
3567 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XcmI
XhoII