##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccI AccIII AgeI Aor51HI
AseI AsnI Asp718I AviII BbrPI
BcgI BclI BglII BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SapI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 4228 GACGTC GACGT^C
AccII 1298 CGCG CG^CG
AflII 2841 CTTAAG C^TTAAG
AhdI 836 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2997 GTGCAC G^TGCAC
ApaLI 2997 GTGCAC G^TGCAC
AscI 1296 GGCGCGCC GG^CGCGCC
Asp700I 1063 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 836 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1264 GGATCC G^GATCC
BbeI 1589 GGCGCC GGCGC^C
BfrI 2841 CTTAAG C^TTAAG
BglI 2201 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3485 CACGTA [CT]AC^GT[GA]
BsiEI 3761 CGGCCG CG[GA][CT]^CG
BspLU11I 4124 ACATGT A^CATGT
BsrBI 931 CCGCTC CCGCTC(-3/-3)
BsrDI 365 GCAATG GCAATG(2/0)
BssHII 1297 GCGCGC G^CGCGC
BstUI 1298 CGCG CG^CG
EagI 3761 CGGCCG C^GGCCG
Eam1105I 836 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3761 CGGCCG C^GGCCG
Eco52I 3761 CGGCCG C^GGCCG
HincII 1248 GTTGAC GT[CT]^[GA]AC
HindII 1248 GTTGAC GT[CT]^[GA]AC
KasI 1589 GGCGCC G^GCGCC
MvnI 1298 CGCG CG^CG
NaeI 1002 GCCGGC GCC^GGC
NarI 1589 GGCGCC GG^CGCC
NgoMIV 1002 GCCGGC G^CCGGC
NheI 1874 GCTAGC G^CTAGC
PflMI 1747 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1515 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 3110 AGTACT AGT^ACT
SexAI 539 ACCAGGT A^CC[AT]GGT
SfoI 1589 GGCGCC GGC^GCC
SphI 2695 GCATGC GCATG^C
Van91I 1747 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1063 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AclI 2821 AACGTT AA^CGTT
4325 AACGTT
AcsI 53 AAATTT [GA]^AATT[CT]
1528 AAATTT
ApoI 53 AAATTT [GA]^AATT[CT]
1528 AAATTT
AspI 2162 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4190 GACTGTGTC
BciVI 1421 GGATAC GTATCC(6/5)
2048 GGATAC
BsaI 591 GAGACC GGTCTC(1/5)
2099 GAGACC
BspHI 199 TCATGA T^CATGA
3995 TCATGA
BssSI 3122 CACGAG CACGAG(-5/-1)
3037 CTCGTG
BstAPI 619 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1796 GCACTACCTGC
BstEII 1521 GGTCACC G^GT[ACGT]ACC
3782 GGTCACC
BstPI 1521 GGTCACC G^GT[ACGT]ACC
3782 GGTCACC
BstXI 1174 CCAGTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3023 CCACCTGAGTGG
EcoO65I 1521 GGTCACC G^GT[ACGT]ACC
3782 GGTCACC
NcoI 1159 CCATGG C^CATGG
1292 CCATGG
PflFI 2162 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4190 GACTGTGTC
Psp1406I 2821 AACGTT AA^CGTT
4325 AACGTT
RcaI 199 TCATGA T^CATGA
3995 TCATGA
SacI 2384 GAGCTC GAGCT^C
3991 GAGCTC
SbfI 3869 CCTGCAGG CCTGCA^GG
3896 CCTGCAGG
SmaI 886 CCCGGG CCC^GGG
1376 CCCGGG
Sse8387I 3869 CCTGCAGG CCTGCA^GG
3896 CCTGCAGG
Tth111I 2162 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4190 GACTGTGTC
XmaCI 886 CCCGGG C^CCGGG
1376 CCCGGG
XmaI 886 CCCGGG C^CCGGG
1376 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII