##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BciVI BclI BfrI BsiWI BspDI
BspHI BssHII Bst1107I BstZ17I ClaI
DraI Eam1105I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI PvuI RcaI SacI
SacII SalI SbfI ScaI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 1839 TCCGGA T^CCGGA
AclI 653 AACGTT AA^CGTT
Alw44I 1896 GTGCAC G^TGCAC
ApaLI 1896 GTGCAC G^TGCAC
BamHI 1753 GGATCC G^GATCC
BbeI 491 GGCGCC GGCGC^C
BbrPI 2891 CACGTG CAC^GTG
BglII 2521 AGATCT A^GATCT
BsaBI 1748 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1839 TCCGGA T^CCGGA
BsiEI 1452 CGGCCG CG[GA][CT]^CG
BsmI 663 GCATTC GAATGC(1/-1)
Bsp1407I 1510 TGTACA T^GTACA
BspEI 1839 TCCGGA T^CCGGA
BspLU11I 187 ACATGT A^CATGT
BsrDI 1589 CATTGC GCAATG(2/0)
BsrGI 1510 TGTACA T^GTACA
BssSI 252 CACGAG CACGAG(-5/-1)
BstBI 657 TTCGAA TT^CGAA
DrdI 3652 GACACCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1452 CGGCCG C^GGCCG
EclXI 1452 CGGCCG C^GGCCG
Eco52I 1452 CGGCCG C^GGCCG
EcoT22I 3577 ATGCAT ATGCA^T
KasI 491 GGCGCC G^GCGCC
MamI 1748 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1839 TCCGGA T^CCGGA
NaeI 1845 GCCGGC GCC^GGC
NarI 491 GGCGCC GG^CGCC
NgoMIV 1845 GCCGGC G^CCGGC
NsiI 3577 ATGCAT ATGCA^T
NspV 657 TTCGAA TT^CGAA
PmaCI 2891 CACGTG CAC^GTG
PmlI 2891 CACGTG CAC^GTG
Psp1406I 653 AACGTT AA^CGTT
SapI 2444 GAAGAGC GCTCTTC(1/4)
SexAI 3381 ACCAGGT A^CC[AT]GGT
SfiI 3185 GGCCGAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 491 GGCGCC GGC^GCC
SfuI 657 TTCGAA TT^CGAA
SmlI 1644 CTCAAG C^T[CT][GA]AG
SphI 1373 GCATGC GCATG^C
SspBI 1510 TGTACA T^GTACA
XcmI 2704 CCAAGCCCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 346 AGCGCT AGC^GCT
1008 AGCGCT
AvrII 79 CCTAGG C^CTAGG
3783 CCTAGG
BalI 777 TGGCCA TGG^CCA
2359 TGGCCA
BcgI 370 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1690 GCACGTCCATCG
BlnI 79 CCTAGG C^CTAGG
3783 CCTAGG
BsaI 7 GGTCTC GGTCTC(1/5)
1447 GGTCTC
BstEII 390 GGTGACC G^GT[ACGT]ACC
2928 GGTCACC
BstPI 390 GGTGACC G^GT[ACGT]ACC
2928 GGTCACC
CpoI 1325 CGGTCCG CG^G[AT]CCG
1537 CGGACCG
EarI 3860 CTCTTC CTCTTC(1/4)
2444 GAAGAG
Eco47III 346 AGCGCT AGC^GCT
1008 AGCGCT
EcoO65I 390 GGTGACC G^GT[ACGT]ACC
2928 GGTCACC
Ksp632I 3860 CTCTTC CTCTTC(1/4)
2444 GAAGAG
MluNI 777 TGGCCA TGG^CCA
2359 TGGCCA
MscI 777 TGGCCA TGG^CCA
2359 TGGCCA
MseI 55 TTAA T^TAA
1047 TTAA
NcoI 2242 CCATGG C^CATGG
2713 CCATGG
PleI 1099 GAGTC GAGTC(4/5)
3633 GAGTC
PpuMI 1182 GGGACCC [GA]G^G[AT]CC[CT]
2038 AGGACCT
RsrII 1325 CGGTCCG CG^G[AT]CCG
1537 CGGACCG
Tru9I 55 TTAA T^TAA
1047 TTAA
Tsp509I 1049 AATT ^AATT
2681 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI