##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII BbrPI BcgI BclI
BfrI BlpI Bpu1102I BsaAI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI EcoRI EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SalI ScaI SfuI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 2717 AAATTC [GA]^AATT[CT]
AhdI 1457 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2717 AAATTC [GA]^AATT[CT]
AspEI 1457 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1510 CCTAGG C^CTAGG
BamHI 436 GGATCC G^GATCC
BciVI 445 GGATAC GTATCC(6/5)
BglII 3870 AGATCT A^GATCT
BlnI 1510 CCTAGG C^CTAGG
BsaBI 3737 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 4294 TCATGA T^CATGA
BssSI 2143 CTCGTG CACGAG(-5/-1)
DraIII 2140 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1457 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 800 GATATC GAT^ATC
MamI 3737 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 4285 TTAA T^TAA
NcoI 123 CCATGG C^CATGG
PflMI 3384 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2821 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4294 TCATGA T^CATGA
SexAI 3918 ACCTGGT A^CC[AT]GGT
SgrAI 1169 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1471 GCATGC GCATG^C
Tru9I 4285 TTAA T^TAA
Van91I 3384 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccIII 853 TCCGGA T^CCGGA
3091 TCCGGA
BsaWI 853 TCCGGA [AT]^CCGG[AT]
3091 TCCGGA
BseAI 853 TCCGGA T^CCGGA
3091 TCCGGA
BsmBI 572 CGTCTC CGTCTC(1/5)
663 GAGACG
BspEI 853 TCCGGA T^CCGGA
3091 TCCGGA
DrdI 154 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3007 GACTTCCTGGTC
MroI 853 TCCGGA T^CCGGA
3091 TCCGGA
MslI 2236 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2776 CACTGAGATG
NspI 495 ACATGC [GA]CATG^[CT]
1471 GCATGC
PaeR7I 144 CTCGAG C^TCGAG
1996 CTCGAG
SapI 2577 GCTCTTC GCTCTTC(1/4)
4076 GCTCTTC
SbfI 262 CCTGCAGG CCTGCA^GG
1498 CCTGCAGG
Sse8387I 262 CCTGCAGG CCTGCA^GG
1498 CCTGCAGG
StuI 831 AGGCCT AGG^CCT
988 AGGCCT
Tsp509I 2718 AATT ^AATT
4278 AATT
XcmI 3423 CCAGCGGCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3877 CCACAGCAAAAGTGG
XhoI 144 CTCGAG C^TCGAG
1996 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI