##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

Acc65I      AclI        AgeI        AocI        Aor51HI     
AscI        Asp718I     AviII       BamHI       BbeI        
BcgI        BglII       BsiEI       BsiWI       Bsp1407I    
BspDI       BsrBI       BsrFI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MspA1I      MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        4182  GACGTC               GACGT^C
AccI         1509  GTCTAC               GT^[AC][GT]AC
AccIII       2019  TCCGGA               T^CCGGA
AcyI         4182  GACGTC               G[GA]^CG[CT]C
AflII        3533  CTTAAG               C^TTAAG
AhdI         4588  GACATGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        628  GTGCAC               G^TGCAC
ApaLI         628  GTGCAC               G^TGCAC
Asp700I       423  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4588  GACATGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        1001  CCTAGG               C^CTAGG
BbrPI        2950  CACGTG               CAC^GTG
BbvCI        1911  CCTCAGC              CCTCAGC(-5/-2)
BfrI         3533  CTTAAG               C^TTAAG
BglI         1671  GCCTGGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1001  CCTAGG               C^CTAGG
BsaAI        2950  CACGTG               [CT]AC^GT[GA]
BsaBI         900  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        4182  GACGTC               G[GA]^CG[CT]C
BseAI        2019  TCCGGA               T^CCGGA
BsmI         1682  GAATGC               GAATGC(1/-1)
BspEI        2019  TCCGGA               T^CCGGA
BssHII       1621  GCGCGC               G^CGCGC
BstAPI       1875  GCAAGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        3101  GGATCT               [GA]^GATC[CT]
Eam1105I     4588  GACATGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         591  GAATTC               G^AATTC
HaeII        1856  GGCGCT               [GA]GCGC^[CT]
HgaI         1591  GACGC                GACGC(5/10)
Hin1I        4182  GACGTC               G[GA]^CG[CT]C
HincII       2787  GTCAAC               GT[CT]^[GA]AC
HindII       2787  GTCAAC               GT[CT]^[GA]AC
MamI          900  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         3101  GGATCT               [GA]^GATC[CT]
MroI         2019  TCCGGA               T^CCGGA
PmaCI        2950  CACGTG               CAC^GTG
PmlI         2950  CACGTG               CAC^GTG
TaqI         2013  TCGA                 T^CGA
TthHB8I      2013  TCGA                 T^CGA
XhoII        3101  GGATCT               [GA]^GATC[CT]
XmnI          423  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII        1622  CGCG                 CG^CG
             2082  CGCG                
AlwI         2031  GATCC                GGATC(4/5)
             3101  GGATC               
AlwNI        2617  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4247  CAGGGTCTG           
AvaI         1920  CCCGAG               C^[CT]CG[GA]G
             2823  CTCGGG              
BalI         2433  TGGCCA               TGG^CCA
             2459  TGGCCA              
BciVI        3397  GGATAC               GTATCC(6/5)
             3128  GTATCC              
BclI         1007  TGATCA               T^GATCA
             3267  TGATCA              
BcnI         2086  CCCGG                CC^[GC]GG
             2660  CCCGG               
BlpI         3315  GCTTAGC              GC^T[ACGT]AGC
             4529  GCTCAGC             
Bpu1102I     3315  GCTTAGC              GC^T[ACGT]AGC
             4529  GCTCAGC             
BsaI          914  GAGACC               GGTCTC(1/5)
              352  GGTCTC              
BsaWI        2019  TCCGGA               [AT]^CCGG[AT]
             2179  TCCGGT              
BsoBI        1920  CCCGAG               C^[CT]CG[GA]G
             2823  CTCGGG              
BspLU11I     2406  ACATGT               A^CATGT
             4427  ACATGT              
BsrDI        1267  CATTGC               GCAATG(2/0)
             4215  GCAATG              
BstUI        1622  CGCG                 CG^CG
             2082  CGCG                
CelII        3315  GCTTAGC              GC^T[ACGT]AGC
             4529  GCTCAGC             
DraI          690  TTTAAA               TTT^AAA
             1452  TTTAAA              
EaeI         2433  TGGCCA               [CT]^GGCC[GA]
             2459  TGGCCA              
FauI         1307  CCCGC                CCCGC(4/6)
             1602  GCGGG               
FbaI         1007  TGATCA               T^GATCA
             3267  TGATCA              
HindIII      1069  AAGCTT               A^AGCTT
             3820  AAGCTT              
MluNI        2433  TGGCCA               TGG^CCA
             2459  TGGCCA              
MscI         2433  TGGCCA               TGG^CCA
             2459  TGGCCA              
MvnI         1622  CGCG                 CG^CG
             2082  CGCG                
NciI         2086  CCCGG                CC^[GC]GG
             2660  CCCGG               
SapI         1492  GAAGAGC              GCTCTTC(1/4)
             2143  GAAGAGC             
SspI         3483  AATATT               AAT^ATT
             3653  AATATT              
StuI          813  AGGCCT               AGG^CCT
             1348  AGGCCT              
XcmI          648  CCATCCCTTGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2389  CCATTTGAGCTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
ApaI        ApoI        AseI        AsnI        AspHI       
AspI        AvaII       BanI        BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       Bsp1286I    
BspHI       BspMI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PshBI       RcaI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
Van91I      XbaI