################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## Acc65I AclI AgeI AocI Aor51HI AscI Asp718I AviII BamHI BbeI BcgI BglII BsiEI BsiWI Bsp1407I BspDI BsrBI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MfeI MluI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 4182 GACGTC GACGT^C AccI 1509 GTCTAC GT^[AC][GT]AC AccIII 2019 TCCGGA T^CCGGA AcyI 4182 GACGTC G[GA]^CG[CT]C AflII 3533 CTTAAG C^TTAAG AhdI 4588 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 628 GTGCAC G^TGCAC ApaLI 628 GTGCAC G^TGCAC Asp700I 423 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4588 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1001 CCTAGG C^CTAGG BbrPI 2950 CACGTG CAC^GTG BbvCI 1911 CCTCAGC CCTCAGC(-5/-2) BfrI 3533 CTTAAG C^TTAAG BglI 1671 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1001 CCTAGG C^CTAGG BsaAI 2950 CACGTG [CT]AC^GT[GA] BsaBI 900 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4182 GACGTC G[GA]^CG[CT]C BseAI 2019 TCCGGA T^CCGGA BsmI 1682 GAATGC GAATGC(1/-1) BspEI 2019 TCCGGA T^CCGGA BssHII 1621 GCGCGC G^CGCGC BstAPI 1875 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 3101 GGATCT [GA]^GATC[CT] Eam1105I 4588 GACATGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 591 GAATTC G^AATTC HaeII 1856 GGCGCT [GA]GCGC^[CT] HgaI 1591 GACGC GACGC(5/10) Hin1I 4182 GACGTC G[GA]^CG[CT]C HincII 2787 GTCAAC GT[CT]^[GA]AC HindII 2787 GTCAAC GT[CT]^[GA]AC MamI 900 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 3101 GGATCT [GA]^GATC[CT] MroI 2019 TCCGGA T^CCGGA PmaCI 2950 CACGTG CAC^GTG PmlI 2950 CACGTG CAC^GTG TaqI 2013 TCGA T^CGA TthHB8I 2013 TCGA T^CGA XhoII 3101 GGATCT [GA]^GATC[CT] XmnI 423 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 1622 CGCG CG^CG 2082 CGCG AlwI 2031 GATCC GGATC(4/5) 3101 GGATC AlwNI 2617 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 4247 CAGGGTCTG AvaI 1920 CCCGAG C^[CT]CG[GA]G 2823 CTCGGG BalI 2433 TGGCCA TGG^CCA 2459 TGGCCA BciVI 3397 GGATAC GTATCC(6/5) 3128 GTATCC BclI 1007 TGATCA T^GATCA 3267 TGATCA BcnI 2086 CCCGG CC^[GC]GG 2660 CCCGG BlpI 3315 GCTTAGC GC^T[ACGT]AGC 4529 GCTCAGC Bpu1102I 3315 GCTTAGC GC^T[ACGT]AGC 4529 GCTCAGC BsaI 914 GAGACC GGTCTC(1/5) 352 GGTCTC BsaWI 2019 TCCGGA [AT]^CCGG[AT] 2179 TCCGGT BsoBI 1920 CCCGAG C^[CT]CG[GA]G 2823 CTCGGG BspLU11I 2406 ACATGT A^CATGT 4427 ACATGT BsrDI 1267 CATTGC GCAATG(2/0) 4215 GCAATG BstUI 1622 CGCG CG^CG 2082 CGCG CelII 3315 GCTTAGC GC^T[ACGT]AGC 4529 GCTCAGC DraI 690 TTTAAA TTT^AAA 1452 TTTAAA EaeI 2433 TGGCCA [CT]^GGCC[GA] 2459 TGGCCA FauI 1307 CCCGC CCCGC(4/6) 1602 GCGGG FbaI 1007 TGATCA T^GATCA 3267 TGATCA HindIII 1069 AAGCTT A^AGCTT 3820 AAGCTT MluNI 2433 TGGCCA TGG^CCA 2459 TGGCCA MscI 2433 TGGCCA TGG^CCA 2459 TGGCCA MvnI 1622 CGCG CG^CG 2082 CGCG NciI 2086 CCCGG CC^[GC]GG 2660 CCCGG SapI 1492 GAAGAGC GCTCTTC(1/4) 2143 GAAGAGC SspI 3483 AATATT AAT^ATT 3653 AATATT StuI 813 AGGCCT AGG^CCT 1348 AGGCCT XcmI 648 CCATCCCTTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2389 CCATTTGAGCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApaI ApoI AseI AsnI AspHI AspI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PshBI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI