##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccIII AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I AvrII
BamHI BciVI BclI BfrI BlnI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EarI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI Ksp632I
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI PvuI
RsrII SalI SapI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3821 GACGTC GACGT^C
Acc65I 1240 GGTACC G^GTACC
AccI 3824 GTCTAC GT^[AC][GT]AC
AclI 1768 AACGTT AA^CGTT
AcsI 1935 GAATTC [GA]^AATT[CT]
AflIII 2359 ACACGT A^C[GA][CT]GT
AhdI 3945 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2828 GATCC GGATC(4/5)
Aor51HI 3415 AGCGCT AGC^GCT
ApoI 1935 GAATTC [GA]^AATT[CT]
Asp718I 1240 GGTACC G^GTACC
AspEI 3945 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1375 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3268 TGGCCA TGG^CCA
BglII 1746 AGATCT A^GATCT
BsaI 1215 GGTCTC GGTCTC(1/5)
BsaWI 1312 ACCGGA [AT]^CCGG[AT]
BsrDI 1333 CATTGC GCAATG(2/0)
BstYI 1746 AGATCT [GA]^GATC[CT]
Eam1105I 3945 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3415 AGCGCT AGC^GCT
EcoRI 1935 GAATTC G^AATTC
FseI 3503 GGCCGGCC GGCCGG^CC
KpnI 1240 GGTACC GGTAC^C
MflI 1746 AGATCT [GA]^GATC[CT]
MluNI 3268 TGGCCA TGG^CCA
MscI 3268 TGGCCA TGG^CCA
NcoI 4513 CCATGG C^CATGG
NheI 670 GCTAGC G^CTAGC
PflFI 1375 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 93 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1768 AACGTT AA^CGTT
SbfI 3509 CCTGCAGG CCTGCA^GG
ScaI 4371 AGTACT AGT^ACT
SexAI 2010 ACCAGGT A^CC[AT]GGT
SfiI 4276 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3509 CCTGCAGG CCTGCA^GG
TfiI 2690 GATTC G^A[AT]TC
Tth111I 1375 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 151 TCTAGA T^CTAGA
XhoII 1746 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 5 CCTGAGG CC^T[ACGT]AGG
4438 CCTGAGG
AviII 496 TGCGCA TGC^GCA
934 TGCGCA
BbeI 1775 GGCGCC GGCGC^C
3858 GGCGCC
BbrPI 2360 CACGTG CAC^GTG
4020 CACGTG
BbsI 1825 GAAGAC GAAGAC(2/6)
1842 GTCTTC
BcgI 4495 CGAGGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1930 GCATAGAATTCG
BpuAI 1825 GAAGAC GAAGAC(2/6)
1842 GTCTTC
BsiEI 2386 CGGCCG CG[GA][CT]^CG
3892 CGGCCG
BspHI 2579 TCATGA T^CATGA
4072 TCATGA
BsrBI 4427 CCGCTC CCGCTC(-3/-3)
751 GAGCGG
BssSI 2154 CTCGTG CACGAG(-5/-1)
2823 CTCGTG
BstAPI 2664 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3156 GCAGATGCTGC
Bsu36I 5 CCTGAGG CC^T[ACGT]AGG
4438 CCTGAGG
DrdI 813 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2616 GACAGAGCAGTC
EagI 2386 CGGCCG C^GGCCG
3892 CGGCCG
EclXI 2386 CGGCCG C^GGCCG
3892 CGGCCG
Eco52I 2386 CGGCCG C^GGCCG
3892 CGGCCG
Eco81I 5 CCTGAGG CC^T[ACGT]AGG
4438 CCTGAGG
FspI 496 TGCGCA TGC^GCA
934 TGCGCA
KasI 1775 GGCGCC G^GCGCC
3858 GGCGCC
KspI 941 CCGCGG CCGC^GG
4456 CCGCGG
NaeI 2384 GCCGGC GCC^GGC
3504 GCCGGC
NarI 1775 GGCGCC GG^CGCC
3858 GGCGCC
NgoMIV 2384 GCCGGC G^CCGGC
3504 GCCGGC
NspI 3837 ACATGC [GA]CATG^[CT]
4154 ACATGC
PaeR7I 764 CTCGAG C^TCGAG
3863 CTCGAG
PflMI 314 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1899 CCAGAGCCTGG
PmaCI 2360 CACGTG CAC^GTG
4020 CACGTG
PmlI 2360 CACGTG CAC^GTG
4020 CACGTG
RcaI 2579 TCATGA T^CATGA
4072 TCATGA
SacII 941 CCGCGG CCGC^GG
4456 CCGCGG
SfoI 1775 GGCGCC GGC^GCC
3858 GGCGCC
Van91I 314 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1899 CCAGAGCCTGG
XhoI 764 CTCGAG C^TCGAG
3863 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI