##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AscI Asp700I Asp718I AspI
AviII BbeI BbsI BclI BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TaqI Tth111I TthHB8I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 4366 AAATTT [GA]^AATT[CT]
AflII 2350 CTTAAG C^TTAAG
AhdI 2681 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3363 GGGCCC GGGCC^C
ApoI 4366 AAATTT [GA]^AATT[CT]
AspEI 2681 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1290 GGATCC G^GATCC
BbrPI 2958 CACGTG CAC^GTG
BbvCI 3255 GCTGAGG CCTCAGC(-5/-2)
BcgI 4208 CGATTAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3553 GGATAC GTATCC(6/5)
BfrI 2350 CTTAAG C^TTAAG
BlpI 2523 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2523 GCTAAGC GC^T[ACGT]AGC
BsaAI 2958 CACGTG [CT]AC^GT[GA]
BstEII 1565 GGTTACC G^GT[ACGT]ACC
BstPI 1565 GGTTACC G^GT[ACGT]ACC
CelII 2523 GCTAAGC GC^T[ACGT]AGC
DraI 4327 TTTAAA TTT^AAA
Eam1105I 2681 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1565 GGTTACC G^GT[ACGT]ACC
FauI 4166 GCGGG CCCGC(4/6)
FseI 3117 GGCCGGCC GGCCGG^CC
HincII 4243 GTTAAC GT[CT]^[GA]AC
HindII 4243 GTTAAC GT[CT]^[GA]AC
HindIII 333 AAGCTT A^AGCTT
HpaI 4243 GTTAAC GTT^AAC
MspA1I 4005 CAGCTG C[AC]G^C[GT]G
NaeI 3118 GCCGGC GCC^GGC
NcoI 3775 CCATGG C^CATGG
NgoMIV 3118 GCCGGC G^CCGGC
NheI 2432 GCTAGC G^CTAGC
PmaCI 2958 CACGTG CAC^GTG
PmlI 2958 CACGTG CAC^GTG
PvuII 4005 CAGCTG CAG^CTG
SmaI 833 CCCGGG CCC^GGG
XbaI 2462 TCTAGA T^CTAGA
XmaCI 833 CCCGGG C^CCGGG
XmaI 833 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 2444 CGCG CG^CG
3111 CGCG
AcyI 2081 GGCGTC G[GA]^CG[CT]C
3923 GGCGTC
Alw44I 683 GTGCAC G^TGCAC
4119 GTGCAC
AocI 2582 CCTCAGG CC^T[ACGT]AGG
3473 CCTCAGG
Aor51HI 2067 AGCGCT AGC^GCT
3170 AGCGCT
ApaLI 683 GTGCAC G^TGCAC
4119 GTGCAC
AseI 1720 ATTAAT AT^TAAT
2792 ATTAAT
AsnI 1720 ATTAAT AT^TAAT
2792 ATTAAT
AvrII 1760 CCTAGG C^CTAGG
1915 CCTAGG
BglII 638 AGATCT A^GATCT
4186 AGATCT
BlnI 1760 CCTAGG C^CTAGG
1915 CCTAGG
BsaHI 2081 GGCGTC G[GA]^CG[CT]C
3923 GGCGTC
BsmI 2707 GAATGC GAATGC(1/-1)
2638 GCATTC
BspHI 720 TCATGA T^CATGA
1834 TCATGA
BsrFI 1498 ACCGGC [GA]^CCGG[CT]
3118 GCCGGC
BstUI 2444 CGCG CG^CG
3111 CGCG
BstXI 2217 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2361 CCAATTATGTGG
Bsu36I 2582 CCTCAGG CC^T[ACGT]AGG
3473 CCTCAGG
Cfr10I 1498 ACCGGC [GA]^CCGG[CT]
3118 GCCGGC
Eco47III 2067 AGCGCT AGC^GCT
3170 AGCGCT
Eco81I 2582 CCTCAGG CC^T[ACGT]AGG
3473 CCTCAGG
HaeII 2067 AGCGCT [GA]GCGC^[CT]
3170 AGCGCT
Hin1I 2081 GGCGTC G[GA]^CG[CT]C
3923 GGCGTC
MvnI 2444 CGCG CG^CG
3111 CGCG
PflMI 112 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1954 CCAGATTATGG
PshBI 1720 ATTAAT AT^TAAT
2792 ATTAAT
RcaI 720 TCATGA T^CATGA
1834 TCATGA
StuI 3070 AGGCCT AGG^CCT
3238 AGGCCT
Van91I 112 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1954 CCAGATTATGG
XcmI 208 CCATCCCTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1300 CCATGCCTTACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII