##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AseI AsnI BbeI BglII
BsaWI BseAI BsiWI BspDI BspEI
BssHII BstBI ClaI CpoI DrdI
EcoT22I FseI HindIII KasI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1671 GGTACC G^GTACC
AccII 512 CGCG CG^CG
AhdI 905 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4143 AGCGCT AGC^GCT
Asp718I 1671 GGTACC G^GTACC
AspEI 905 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 496 CCTAGG C^CTAGG
BcgI 1402 CGACAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1494 GTATCC GTATCC(6/5)
BlnI 496 CCTAGG C^CTAGG
BsaBI 2497 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1588 CGGCCG CG[GA][CT]^CG
BspHI 1123 TCATGA T^CATGA
BsrFI 2357 ACCGGC [GA]^CCGG[CT]
BssSI 1958 CTCGTG CACGAG(-5/-1)
Bst1107I 3838 GTATAC GTA^TAC
BstUI 512 CGCG CG^CG
BstZ17I 3838 GTATAC GTA^TAC
Cfr10I 2357 ACCGGC [GA]^CCGG[CT]
DraI 2677 TTTAAA TTT^AAA
EagI 1588 CGGCCG C^GGCCG
Eam1105I 905 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1588 CGGCCG C^GGCCG
Eco47III 4143 AGCGCT AGC^GCT
Eco52I 1588 CGGCCG C^GGCCG
EcoRV 1934 GATATC GAT^ATC
HpaI 1810 GTTAAC GTT^AAC
KpnI 1671 GGTACC GGTAC^C
MamI 2497 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 512 CGCG CG^CG
NcoI 2115 CCATGG C^CATGG
PaeR7I 1730 CTCGAG C^TCGAG
RcaI 1123 TCATGA T^CATGA
SacI 374 GAGCTC GAGCT^C
SapI 1210 GCTCTTC GCTCTTC(1/4)
ScaI 4547 AGTACT AGT^ACT
SspI 1149 AATATT AAT^ATT
XbaI 2767 TCTAGA T^CTAGA
XhoI 1730 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 94 GGCGTC G[GA]^CG[CT]C
4073 GACGCC
AflII 1000 CTTAAG C^TTAAG
1307 CTTAAG
Alw44I 134 GTGCAC G^TGCAC
737 GTGCAC
ApaLI 134 GTGCAC G^TGCAC
737 GTGCAC
AspI 710 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1619 GACGTTGTC
AviII 618 TGCGCA TGC^GCA
2631 TGCGCA
BclI 3865 TGATCA T^GATCA
4390 TGATCA
BfrI 1000 CTTAAG C^TTAAG
1307 CTTAAG
BsaHI 94 GGCGTC G[GA]^CG[CT]C
4073 GACGCC
BsaI 2603 GAGACC GGTCTC(1/5)
3530 GAGACC
BsmBI 2388 GAGACG CGTCTC(1/5)
3224 GAGACG
BspLU11I 2399 ACATGT A^CATGT
4516 ACATGT
BstEII 535 GGTGACC G^GT[ACGT]ACC
2852 GGTGACC
BstPI 535 GGTGACC G^GT[ACGT]ACC
2852 GGTGACC
DraIII 1517 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2954 CACATTGTG
EcoO65I 535 GGTGACC G^GT[ACGT]ACC
2852 GGTGACC
EcoRI 818 GAATTC G^AATTC
4443 GAATTC
FbaI 3865 TGATCA T^GATCA
4390 TGATCA
FspI 618 TGCGCA TGC^GCA
2631 TGCGCA
Hin1I 94 GGCGTC G[GA]^CG[CT]C
4073 GACGCC
PflFI 710 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1619 GACGTTGTC
PshAI 560 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1072 GACTCCTGTC
SphI 865 GCATGC GCATG^C
2122 GCATGC
Tth111I 710 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1619 GACGTTGTC
XcmI 238 CCATGAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2278 CCATGCGCCGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI