##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
AscI AseI AsnI AvaI AviII
BamHI BbvCI BclI BseAI BsiWI
BsoBI BspDI BspEI BspLU11I BsrFI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII Eco81I
EcoRV FbaI FseI FspI KspI
MluI MroI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2830 GGTACC G^GTACC
AhdI 1799 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3967 GTGCAC G^TGCAC
ApaI 3238 GGGCCC GGGCC^C
ApaLI 3967 GTGCAC G^TGCAC
Asp718I 2830 GGTACC G^GTACC
AspEI 1799 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3967 GTGCAC G[AT]GC[AT]^C
AvrII 3702 CCTAGG C^CTAGG
BanII 3238 GGGCCC G[GA]GC[CT]^C
BbeI 1601 GGCGCC GGCGC^C
BbrPI 3964 CACGTG CAC^GTG
BcgI 57 CGAGGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1190 GGATAC GTATCC(6/5)
BlnI 3702 CCTAGG C^CTAGG
BsaBI 2180 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3967 GTGCAC G[AT]GC[AT]^C
BspHI 796 TCATGA T^CATGA
BstEII 1367 GGTAACC G^GT[ACGT]ACC
BstPI 1367 GGTAACC G^GT[ACGT]ACC
DraI 818 TTTAAA TTT^AAA
DrdI 2471 GACCAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2407 CGGCCG C^GGCCG
Eam1105I 1799 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2407 CGGCCG C^GGCCG
Eco52I 2407 CGGCCG C^GGCCG
EcoNI 2004 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1367 GGTAACC G^GT[ACGT]ACC
EcoT22I 3876 ATGCAT ATGCA^T
HincII 3469 GTTAAC GT[CT]^[GA]AC
HindII 3469 GTTAAC GT[CT]^[GA]AC
HpaI 3469 GTTAAC GTT^AAC
KasI 1601 GGCGCC G^GCGCC
KpnI 2830 GGTACC GGTAC^C
MamI 2180 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3511 CAATTG C^AATTG
MunI 3511 CAATTG C^AATTG
NarI 1601 GGCGCC GG^CGCC
NdeI 143 CATATG CA^TATG
NsiI 3876 ATGCAT ATGCA^T
PmaCI 3964 CACGTG CAC^GTG
PmlI 3964 CACGTG CAC^GTG
PstI 1898 CTGCAG CTGCA^G
RcaI 796 TCATGA T^CATGA
SexAI 4128 ACCAGGT A^CC[AT]GGT
SfiI 3150 GGCCAGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1601 GGCGCC GGC^GCC
SphI 3563 GCATGC GCATG^C
StuI 3158 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 289 GTCTAC GT^[AC][GT]AC
2783 GTAGAC
AflII 1292 CTTAAG C^TTAAG
3549 CTTAAG
AflIII 3963 ACACGT A^C[GA][CT]GT
4229 ACACGT
Aor51HI 2052 AGCGCT AGC^GCT
2680 AGCGCT
AspI 1324 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2246 GACACTGTC
BalI 2605 TGGCCA TGG^CCA
3149 TGGCCA
BfrI 1292 CTTAAG C^TTAAG
3549 CTTAAG
BglII 886 AGATCT A^GATCT
2185 AGATCT
BlpI 959 GCTCAGC GC^T[ACGT]AGC
3355 GCTGAGC
Bpu1102I 959 GCTCAGC GC^T[ACGT]AGC
3355 GCTGAGC
BsaI 1058 GAGACC GGTCTC(1/5)
2129 GAGACC
BsaWI 916 ACCGGA [AT]^CCGG[AT]
2600 TCCGGT
BsiEI 2407 CGGCCG CG[GA][CT]^CG
3225 CGGTCG
Bsp1407I 3668 TGTACA T^GTACA
4318 TGTACA
BspMI 3099 ACCTGC ACCTGC(4/8)
2453 GCAGGT
BsrBI 1640 CCGCTC CCGCTC(-3/-3)
2483 CCGCTC
BsrGI 3668 TGTACA T^GTACA
4318 TGTACA
BstBI 1208 TTCGAA TT^CGAA
2539 TTCGAA
CelII 959 GCTCAGC GC^T[ACGT]AGC
3355 GCTGAGC
Eco47III 2052 AGCGCT AGC^GCT
2680 AGCGCT
HindIII 992 AAGCTT A^AGCTT
3433 AAGCTT
MluNI 2605 TGGCCA TGG^CCA
3149 TGGCCA
MscI 2605 TGGCCA TGG^CCA
3149 TGGCCA
NspI 2389 GCATGT [GA]CATG^[CT]
3563 GCATGC
NspV 1208 TTCGAA TT^CGAA
2539 TTCGAA
PflFI 1324 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2246 GACACTGTC
PflMI 487 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4397 CCAAATGATGG
SapI 329 GAAGAGC GCTCTTC(1/4)
515 GAAGAGC
SfuI 1208 TTCGAA TT^CGAA
2539 TTCGAA
SspBI 3668 TGTACA T^GTACA
4318 TGTACA
Tth111I 1324 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2246 GACACTGTC
Van91I 487 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4397 CCAAATGATGG
XbaI 2188 TCTAGA T^CTAGA
3986 TCTAGA
XcmI 945 CCAAAAGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1596 CCAAGGGCGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AvaII BanI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI