##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspI BcgI BclI BfrI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrGI Bst1107I BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoRV EcoT22I
FbaI HincII HindII HindIII HpaI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SexAI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3306 GTCTAC GT^[AC][GT]AC
AccIII 2665 TCCGGA T^CCGGA
AcsI 4175 GAATTC [GA]^AATT[CT]
AflIII 674 ACGTGT A^C[GA][CT]GT
ApoI 4175 GAATTC [GA]^AATT[CT]
AviII 34 TGCGCA TGC^GCA
BamHI 1755 GGATCC G^GATCC
BbeI 2511 GGCGCC GGCGC^C
BciVI 2625 GGATAC GTATCC(6/5)
BglII 3121 AGATCT A^GATCT
BlpI 4062 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4062 GCTGAGC GC^T[ACGT]AGC
BsaBI 213 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2665 TCCGGA T^CCGGA
BsmBI 1198 CGTCTC CGTCTC(1/5)
BspEI 2665 TCCGGA T^CCGGA
BspHI 1866 TCATGA T^CATGA
BssHII 174 GCGCGC G^CGCGC
BstBI 1743 TTCGAA TT^CGAA
CelII 4062 GCTGAGC GC^T[ACGT]AGC
EcoRI 4175 GAATTC G^AATTC
FseI 2603 GGCCGGCC GGCCGG^CC
FspI 34 TGCGCA TGC^GCA
KasI 2511 GGCGCC G^GCGCC
MamI 213 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2665 TCCGGA T^CCGGA
MseI 4339 TTAA T^TAA
NarI 2511 GGCGCC GG^CGCC
NspV 1743 TTCGAA TT^CGAA
PaeR7I 1091 CTCGAG C^TCGAG
RcaI 1866 TCATGA T^CATGA
SbfI 2843 CCTGCAGG CCTGCA^GG
ScaI 3045 AGTACT AGT^ACT
SfoI 2511 GGCGCC GGC^GCC
SfuI 1743 TTCGAA TT^CGAA
Sse8387I 2843 CCTGCAGG CCTGCA^GG
Tru9I 4339 TTAA T^TAA
XhoI 1091 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BbrPI 673 CACGTG CAC^GTG
2421 CACGTG
BbsI 68 GAAGAC GAAGAC(2/6)
2152 GAAGAC
BbvCI 2972 CCTCAGC CCTCAGC(-5/-2)
3800 CCTCAGC
BpuAI 68 GAAGAC GAAGAC(2/6)
2152 GAAGAC
BsaAI 673 CACGTG [CT]AC^GT[GA]
2421 CACGTG
BsaI 1889 GAGACC GGTCTC(1/5)
2340 GAGACC
BsaWI 2665 TCCGGA [AT]^CCGG[AT]
4319 ACCGGA
BstEII 2648 GGTGACC G^GT[ACGT]ACC
4159 GGTGACC
BstPI 2648 GGTGACC G^GT[ACGT]ACC
4159 GGTGACC
BstXI 1524 CCAGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3223 CCACATCGCTGG
EcoO65I 2648 GGTGACC G^GT[ACGT]ACC
4159 GGTGACC
NcoI 1845 CCATGG C^CATGG
3502 CCATGG
PleI 2886 GACTC GAGTC(4/5)
1360 GAGTC
PmaCI 673 CACGTG CAC^GTG
2421 CACGTG
PmlI 673 CACGTG CAC^GTG
2421 CACGTG
PvuII 1683 CAGCTG CAG^CTG
3913 CAGCTG
SfiI 2128 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3782 GGCCGCCGTGGCC
SmaI 1470 CCCGGG CCC^GGG
3095 CCCGGG
SmlI 1091 CTCGAG C^T[CT][GA]AG
4282 CTCAAG
TfiI 1741 GATTC G^A[AT]TC
3698 GATTC
Tsp509I 427 AATT ^AATT
4176 AATT
XmaCI 1470 CCCGGG C^CCGGG
3095 CCCGGG
XmaI 1470 CCCGGG C^CCGGG
3095 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AatII Acc65I AccII AciI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApaI Asp718I AspEI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbvI BcnI BfaI BglI
BlnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PpuMI PstI RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII