##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BcgI BclI
BfrI BglII BlnI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoRI
EcoT22I FbaI FseI FspI HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SfuI SnaBI SpeI SspBI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 1807 GGTACC G^GTACC
Aor51HI 4230 AGCGCT AGC^GCT
Asp718I 1807 GGTACC G^GTACC
BbrPI 3503 CACGTG CAC^GTG
BciVI 403 GGATAC GTATCC(6/5)
BspLU11I 976 ACATGT A^CATGT
DraIII 1554 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4324 GACCCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4230 AGCGCT AGC^GCT
EcoRV 1717 GATATC GAT^ATC
HindIII 1797 AAGCTT A^AGCTT
KpnI 1807 GGTACC GGTAC^C
NruI 3362 TCGCGA TCG^CGA
PflMI 2865 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3503 CACGTG CAC^GTG
PmlI 3503 CACGTG CAC^GTG
SbfI 1130 CCTGCAGG CCTGCA^GG
ScaI 2263 AGTACT AGT^ACT
SexAI 1222 ACCTGGT A^CC[AT]GGT
SgrAI 4366 CGCCGGCG C[GA]^CCGG[CT]G
SphI 710 GCATGC GCATG^C
Sse8387I 1130 CCTGCAGG CCTGCA^GG
SspI 1843 AATATT AAT^ATT
Van91I 2865 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 3577 TCCGGA T^CCGGA
3933 TCCGGA
AflIII 976 ACATGT A^C[GA][CT]GT
3762 ACGTGT
BsaWI 3577 TCCGGA [AT]^CCGG[AT]
3933 TCCGGA
BseAI 3577 TCCGGA T^CCGGA
3933 TCCGGA
BspEI 3577 TCCGGA T^CCGGA
3933 TCCGGA
BspHI 198 TCATGA T^CATGA
3066 TCATGA
BspMI 2776 ACCTGC ACCTGC(4/8)
3519 ACCTGC
BsrBI 4050 CCGCTC CCGCTC(-3/-3)
4142 GAGCGG
BssHII 3483 GCGCGC G^CGCGC
4345 GCGCGC
BstEII 1154 GGTGACC G^GT[ACGT]ACC
1705 GGTGACC
BstPI 1154 GGTGACC G^GT[ACGT]ACC
1705 GGTGACC
BstXI 843 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1338 CCAGGGGTCTGG
EcoO65I 1154 GGTGACC G^GT[ACGT]ACC
1705 GGTGACC
HincII 3449 GTCAAC GT[CT]^[GA]AC
3911 GTCAAC
HindII 3449 GTCAAC GT[CT]^[GA]AC
3911 GTCAAC
KspI 3024 CCGCGG CCGC^GG
3369 CCGCGG
MroI 3577 TCCGGA T^CCGGA
3933 TCCGGA
MseI 2381 TTAA T^TAA
2592 TTAA
MslI 199 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3061 CATCGTCATG
NcoI 736 CCATGG C^CATGG
3591 CCATGG
PshAI 2206 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3749 GACACAAGTC
RcaI 198 TCATGA T^CATGA
3066 TCATGA
SacII 3024 CCGCGG CCGC^GG
3369 CCGCGG
Tru9I 2381 TTAA T^TAA
2592 TTAA
Tsp509I 1480 AATT ^AATT
2287 AATT
XcmI 18 CCACGGCCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1711 CCAGGAGATATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PaeR7I PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoI XhoII XmaCI XmaI