##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BsrDI
BsrGI Bst1107I BstBI BstZ17I ClaI
DraI Eam1105I EcoRV EcoT22I FbaI
HindIII HpaI KpnI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI SalI SapI ScaI
SfiI SfuI SmlI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AatII 1294 GACGTC GACGT^C
Aor51HI 848 AGCGCT AGC^GCT
Asp700I 4261 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3185 GGATCC G^GATCC
BsaBI 2313 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 757 GAGACG CGTCTC(1/5)
BspLU11I 4222 ACATGT A^CATGT
BssHII 1087 GCGCGC G^CGCGC
BssSI 2767 CACGAG CACGAG(-5/-1)
BstEII 445 GGTTACC G^GT[ACGT]ACC
BstPI 445 GGTTACC G^GT[ACGT]ACC
CpoI 136 CGGACCG CG^G[AT]CCG
DrdI 2938 GACGCGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 848 AGCGCT AGC^GCT
EcoO65I 445 GGTTACC G^GT[ACGT]ACC
EcoRI 3019 GAATTC G^AATTC
FseI 277 GGCCGGCC GGCCGG^CC
MamI 2313 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1352 CCATGG C^CATGG
RsrII 136 CGGACCG CG^G[AT]CCG
SbfI 1119 CCTGCAGG CCTGCA^GG
SexAI 728 ACCAGGT A^CC[AT]GGT
SgrAI 918 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1091 GCATGC GCATG^C
Sse8387I 1119 CCTGCAGG CCTGCA^GG
XmnI 4261 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 3019 GAATTC [GA]^AATT[CT]
4262 AAATTT
AflIII 3309 ACGTGT A^C[GA][CT]GT
4222 ACATGT
AocI 2873 CCTTAGG CC^T[ACGT]AGG
3675 CCTCAGG
ApoI 3019 GAATTC [GA]^AATT[CT]
4262 AAATTT
AviII 1664 TGCGCA TGC^GCA
1733 TGCGCA
BbvCI 300 CCTCAGC CCTCAGC(-5/-2)
3682 CCTCAGC
BcgI 465 CGAGCACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2769 CGAGCTCTCTGC
BfaI 3742 CTAG C^TAG
3802 CTAG
BlpI 844 GCTCAGC GC^T[ACGT]AGC
2682 GCTCAGC
Bpu1102I 844 GCTCAGC GC^T[ACGT]AGC
2682 GCTCAGC
BsmI 1067 GAATGC GAATGC(1/-1)
713 GCATTC
Bsu36I 2873 CCTTAGG CC^T[ACGT]AGG
3675 CCTCAGG
CelII 844 GCTCAGC GC^T[ACGT]AGC
2682 GCTCAGC
Eco81I 2873 CCTTAGG CC^T[ACGT]AGG
3675 CCTCAGG
FspI 1664 TGCGCA TGC^GCA
1733 TGCGCA
HincII 2037 GTTGAC GT[CT]^[GA]AC
2719 GTCAAC
HindII 2037 GTTGAC GT[CT]^[GA]AC
2719 GTCAAC
MaeI 3742 CTAG C^TAG
3802 CTAG
NspI 1091 GCATGC [GA]CATG^[CT]
4222 ACATGT
SacI 2437 GAGCTC GAGCT^C
2770 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI