##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AocI Aor51HI AscI AspI AvaI
BbrPI BcgI BlpI Bpu1102I BseAI
BsiWI BsoBI Bsp1407I BspEI BspHI
BsrBI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII CpoI DraIII
Eco47III Eco81I EcoRI EcoRV EcoT22I
FseI HpaI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmlI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1722 GGTACC G^GTACC
AcyI 192 GGCGCC G[GA]^CG[CT]C
AflII 4092 CTTAAG C^TTAAG
AflIII 126 ACATGT A^C[GA][CT]GT
ApaI 3194 GGGCCC GGGCC^C
Asp700I 2773 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1722 GGTACC G^GTACC
AviII 570 TGCGCA TGC^GCA
AvrII 3779 CCTAGG C^CTAGG
BamHI 2413 GGATCC G^GATCC
BbeI 192 GGCGCC GGCGC^C
BciVI 1689 GGATAC GTATCC(6/5)
BfrI 4092 CTTAAG C^TTAAG
BglI 2696 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3779 CCTAGG C^CTAGG
BsaHI 192 GGCGCC G[GA]^CG[CT]C
BsaWI 2081 ACCGGA [AT]^CCGG[AT]
BspLU11I 126 ACATGT A^CATGT
BssSI 326 CACGAG CACGAG(-5/-1)
BstEII 697 GGTCACC G^GT[ACGT]ACC
BstPI 697 GGTCACC G^GT[ACGT]ACC
DsaI 3723 CCATGG C^C[GA][CT]GG
EagI 72 CGGCCG C^GGCCG
EclXI 72 CGGCCG C^GGCCG
Eco52I 72 CGGCCG C^GGCCG
EcoNI 1606 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 697 GGTCACC G^GT[ACGT]ACC
FspI 570 TGCGCA TGC^GCA
Hin1I 192 GGCGCC G[GA]^CG[CT]C
HincII 1768 GTCAAC GT[CT]^[GA]AC
HindII 1768 GTCAAC GT[CT]^[GA]AC
KasI 192 GGCGCC G^GCGCC
KpnI 1722 GGTACC GGTAC^C
NaeI 29 GCCGGC GCC^GGC
NarI 192 GGCGCC GG^CGCC
NcoI 3723 CCATGG C^CATGG
NgoMIV 29 GCCGGC G^CCGGC
PmeI 1561 GTTTAAAC GTTT^AAAC
PpuMI 2912 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 3060 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2885 AGTACT AGT^ACT
SfoI 192 GGCGCC GGC^GCC
SnaBI 3868 TACGTA TAC^GTA
SphI 3658 GCATGC GCATG^C
StuI 1614 AGGCCT AGG^CCT
XmnI 2773 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 143 CGCG CG^CG
2057 CGCG
AhdI 125 GACATGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1649 GACGCTCTGTC
Alw44I 2648 GTGCAC G^TGCAC
3649 GTGCAC
ApaLI 2648 GTGCAC G^TGCAC
3649 GTGCAC
AseI 1358 ATTAAT AT^TAAT
2367 ATTAAT
AsnI 1358 ATTAAT AT^TAAT
2367 ATTAAT
AspEI 125 GACATGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1649 GACGCTCTGTC
BbsI 1744 GTCTTC GAAGAC(2/6)
2689 GTCTTC
BbvCI 102 GCTGAGG CCTCAGC(-5/-2)
2728 GCTGAGG
BglII 265 AGATCT A^GATCT
2224 AGATCT
BpuAI 1744 GTCTTC GAAGAC(2/6)
2689 GTCTTC
BsaBI 1690 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1981 GATGATTATC
BsgI 2436 CTGCAC GTGCAG(16/14)
1777 GTGCAG
BsiEI 72 CGGCCG CG[GA][CT]^CG
2079 CGACCG
BsmBI 551 GAGACG CGTCTC(1/5)
1647 GAGACG
BsmI 997 GAATGC GAATGC(1/-1)
33 GCATTC
BspDI 1988 ATCGAT AT^CGAT
3622 ATCGAT
BsrDI 2423 GCAATG GCAATG(2/0)
2445 GCAATG
BsrFI 29 GCCGGC [GA]^CCGG[CT]
201 ACCGGC
BstAPI 512 GCAAAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2719 GCAGGCTCTGC
BstUI 143 CGCG CG^CG
2057 CGCG
Cfr10I 29 GCCGGC [GA]^CCGG[CT]
201 ACCGGC
ClaI 1988 ATCGAT AT^CGAT
3622 ATCGAT
DrdI 842 GACATTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3105 GACGCTGCGGTC
Eam1105I 125 GACATGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1649 GACGCTCTGTC
MamI 1690 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1981 GATGATTATC
MvnI 143 CGCG CG^CG
2057 CGCG
PshBI 1358 ATTAAT AT^TAAT
2367 ATTAAT
SacI 16 GAGCTC GAGCT^C
2572 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII