##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AccII       AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        AspEI       
AspI        AviII       BamHI       BbeI        BcgI        
BciVI       BfrI        BglI        BsaBI       BsiEI       
BsiWI       BsmBI       BspDI       BspHI       BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     ClaI        
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       KasI        
KspI        MamI        MluI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       PvuI        
RcaI        RsrII       SacII       SalI        SapI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       2864  GGTACC               G^GTACC
AccI         3772  GTCTAC               GT^[AC][GT]AC
AclI          792  AACGTT               AA^CGTT
AcyI         1946  GACGCC               G[GA]^CG[CT]C
AflIII       3730  ACATGT               A^C[GA][CT]GT
Alw44I       3255  GTGCAC               G^TGCAC
ApaI         3305  GGGCCC               GGGCC^C
ApaLI        3255  GTGCAC               G^TGCAC
Asp718I      2864  GGTACC               G^GTACC
AvaI          305  CCCGGG               C^[CT]CG[GA]G
AvrII        1866  CCTAGG               C^CTAGG
BbrPI         267  CACGTG               CAC^GTG
BglII         527  AGATCT               A^GATCT
BlnI         1866  CCTAGG               C^CTAGG
BlpI          244  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      244  GCTCAGC              GC^T[ACGT]AGC
BsaAI         267  CACGTG               [CT]AC^GT[GA]
BsaHI        1946  GACGCC               G[GA]^CG[CT]C
BsoBI         305  CCCGGG               C^[CT]CG[GA]G
BspLU11I     3730  ACATGT               A^CATGT
BsrFI         542  GCCGGT               [GA]^CCGG[CT]
CelII         244  GCTCAGC              GC^T[ACGT]AGC
CfoI          116  GCGC                 GCG^C
Cfr10I        542  GCCGGT               [GA]^CCGG[CT]
DraIII        267  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1675  CCACGG               C^C[GA][CT]GG
EarI          322  CTCTTC               CTCTTC(1/4)
HgaI         1946  GACGC                GACGC(5/10)
HhaI          116  GCGC                 GCG^C
Hin1I        1946  GACGCC               G[GA]^CG[CT]C
HinP1I        116  GCGC                 G^CGC
HindIII      1669  AAGCTT               A^AGCTT
HpaI          502  GTTAAC               GTT^AAC
KpnI         2864  GGTACC               GGTAC^C
Ksp632I       322  CTCTTC               CTCTTC(1/4)
MfeI         1140  CAATTG               C^AATTG
MunI         1140  CAATTG               C^AATTG
PmaCI         267  CACGTG               CAC^GTG
PmlI          267  CACGTG               CAC^GTG
PpuMI        3736  GGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I      792  AACGTT               AA^CGTT
SacI         2443  GAGCTC               GAGCT^C
SbfI          342  CCTGCAGG             CCTGCA^GG
SmaI          305  CCCGGG               CCC^GGG
SphI         3633  GCATGC               GCATG^C
Sse8387I      342  CCTGCAGG             CCTGCA^GG
XbaI          574  TCTAGA               T^CTAGA
XcmI         3291  CCACTGTTTTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         305  CCCGGG               C^CCGGG
XmaI          305  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        209  TCCGGA               T^CCGGA
             3448  TCCGGA              
AlwNI        3686  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3941  CAGACACTG           
AocI         2082  CCTAAGG              CC^T[ACGT]AGG
             2566  CCTGAGG             
BanI         2679  GGCACC               G^G[CT][GA]CC
             2864  GGTACC              
BbsI          352  GTCTTC               GAAGAC(2/6)
             1171  GTCTTC              
BclI         3911  TGATCA               T^GATCA
             4002  TGATCA              
BpuAI         352  GTCTTC               GAAGAC(2/6)
             1171  GTCTTC              
BseAI         209  TCCGGA               T^CCGGA
             3448  TCCGGA              
Bsp1407I     1775  TGTACA               T^GTACA
             4136  TGTACA              
BspEI         209  TCCGGA               T^CCGGA
             3448  TCCGGA              
BsrDI        2713  CATTGC               GCAATG(2/0)
              702  GCAATG              
BsrGI        1775  TGTACA               T^GTACA
             4136  TGTACA              
BstXI        2049  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2611  CCATCCAACTGG        
BstYI         527  AGATCT               [GA]^GATC[CT]
             2542  AGATCC              
Bsu36I       2082  CCTAAGG              CC^T[ACGT]AGG
             2566  CCTGAGG             
DraI          232  TTTAAA               TTT^AAA
             3425  TTTAAA              
Eco81I       2082  CCTAAGG              CC^T[ACGT]AGG
             2566  CCTGAGG             
FauI           59  CCCGC                CCCGC(4/6)
             2480  GCGGG               
FbaI         3911  TGATCA               T^GATCA
             4002  TGATCA              
MaeII         268  ACGT                 A^CGT
              793  ACGT                
MflI          527  AGATCT               [GA]^GATC[CT]
             2542  AGATCC              
MroI          209  TCCGGA               T^CCGGA
             3448  TCCGGA              
NdeI          726  CATATG               CA^TATG
             4250  CATATG              
PshAI        1922  GACGAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2398  GACCTGGGTC          
SexAI        2201  ACCTGGT              A^CC[AT]GGT
             2860  ACCTGGT             
SspBI        1775  TGTACA               T^GTACA
             4136  TGTACA              
XhoII         527  AGATCT               [GA]^GATC[CT]
             2542  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BalI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XmnI