##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII BamHI BbeI BcgI
BciVI BfrI BglI BsaBI BsiEI
BsiWI BsmBI BspDI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII KasI
KspI MamI MluI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
RcaI RsrII SacII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2864 GGTACC G^GTACC
AccI 3772 GTCTAC GT^[AC][GT]AC
AclI 792 AACGTT AA^CGTT
AcyI 1946 GACGCC G[GA]^CG[CT]C
AflIII 3730 ACATGT A^C[GA][CT]GT
Alw44I 3255 GTGCAC G^TGCAC
ApaI 3305 GGGCCC GGGCC^C
ApaLI 3255 GTGCAC G^TGCAC
Asp718I 2864 GGTACC G^GTACC
AvaI 305 CCCGGG C^[CT]CG[GA]G
AvrII 1866 CCTAGG C^CTAGG
BbrPI 267 CACGTG CAC^GTG
BglII 527 AGATCT A^GATCT
BlnI 1866 CCTAGG C^CTAGG
BlpI 244 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 244 GCTCAGC GC^T[ACGT]AGC
BsaAI 267 CACGTG [CT]AC^GT[GA]
BsaHI 1946 GACGCC G[GA]^CG[CT]C
BsoBI 305 CCCGGG C^[CT]CG[GA]G
BspLU11I 3730 ACATGT A^CATGT
BsrFI 542 GCCGGT [GA]^CCGG[CT]
CelII 244 GCTCAGC GC^T[ACGT]AGC
CfoI 116 GCGC GCG^C
Cfr10I 542 GCCGGT [GA]^CCGG[CT]
DraIII 267 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1675 CCACGG C^C[GA][CT]GG
EarI 322 CTCTTC CTCTTC(1/4)
HgaI 1946 GACGC GACGC(5/10)
HhaI 116 GCGC GCG^C
Hin1I 1946 GACGCC G[GA]^CG[CT]C
HinP1I 116 GCGC G^CGC
HindIII 1669 AAGCTT A^AGCTT
HpaI 502 GTTAAC GTT^AAC
KpnI 2864 GGTACC GGTAC^C
Ksp632I 322 CTCTTC CTCTTC(1/4)
MfeI 1140 CAATTG C^AATTG
MunI 1140 CAATTG C^AATTG
PmaCI 267 CACGTG CAC^GTG
PmlI 267 CACGTG CAC^GTG
PpuMI 3736 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 792 AACGTT AA^CGTT
SacI 2443 GAGCTC GAGCT^C
SbfI 342 CCTGCAGG CCTGCA^GG
SmaI 305 CCCGGG CCC^GGG
SphI 3633 GCATGC GCATG^C
Sse8387I 342 CCTGCAGG CCTGCA^GG
XbaI 574 TCTAGA T^CTAGA
XcmI 3291 CCACTGTTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 305 CCCGGG C^CCGGG
XmaI 305 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 209 TCCGGA T^CCGGA
3448 TCCGGA
AlwNI 3686 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3941 CAGACACTG
AocI 2082 CCTAAGG CC^T[ACGT]AGG
2566 CCTGAGG
BanI 2679 GGCACC G^G[CT][GA]CC
2864 GGTACC
BbsI 352 GTCTTC GAAGAC(2/6)
1171 GTCTTC
BclI 3911 TGATCA T^GATCA
4002 TGATCA
BpuAI 352 GTCTTC GAAGAC(2/6)
1171 GTCTTC
BseAI 209 TCCGGA T^CCGGA
3448 TCCGGA
Bsp1407I 1775 TGTACA T^GTACA
4136 TGTACA
BspEI 209 TCCGGA T^CCGGA
3448 TCCGGA
BsrDI 2713 CATTGC GCAATG(2/0)
702 GCAATG
BsrGI 1775 TGTACA T^GTACA
4136 TGTACA
BstXI 2049 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2611 CCATCCAACTGG
BstYI 527 AGATCT [GA]^GATC[CT]
2542 AGATCC
Bsu36I 2082 CCTAAGG CC^T[ACGT]AGG
2566 CCTGAGG
DraI 232 TTTAAA TTT^AAA
3425 TTTAAA
Eco81I 2082 CCTAAGG CC^T[ACGT]AGG
2566 CCTGAGG
FauI 59 CCCGC CCCGC(4/6)
2480 GCGGG
FbaI 3911 TGATCA T^GATCA
4002 TGATCA
MaeII 268 ACGT A^CGT
793 ACGT
MflI 527 AGATCT [GA]^GATC[CT]
2542 AGATCC
MroI 209 TCCGGA T^CCGGA
3448 TCCGGA
NdeI 726 CATATG CA^TATG
4250 CATATG
PshAI 1922 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2398 GACCTGGGTC
SexAI 2201 ACCTGGT A^CC[AT]GGT
2860 ACCTGGT
SspBI 1775 TGTACA T^GTACA
4136 TGTACA
XhoII 527 AGATCT [GA]^GATC[CT]
2542 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BalI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmnI