################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccII AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII BamHI BbeI BcgI BciVI BfrI BglI BsaBI BsiEI BsiWI BsmBI BspDI BspHI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII KasI KspI MamI MluI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI PvuI RcaI RsrII SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 2864 GGTACC G^GTACC AccI 3772 GTCTAC GT^[AC][GT]AC AclI 792 AACGTT AA^CGTT AcyI 1946 GACGCC G[GA]^CG[CT]C AflIII 3730 ACATGT A^C[GA][CT]GT Alw44I 3255 GTGCAC G^TGCAC ApaI 3305 GGGCCC GGGCC^C ApaLI 3255 GTGCAC G^TGCAC Asp718I 2864 GGTACC G^GTACC AvaI 305 CCCGGG C^[CT]CG[GA]G AvrII 1866 CCTAGG C^CTAGG BbrPI 267 CACGTG CAC^GTG BglII 527 AGATCT A^GATCT BlnI 1866 CCTAGG C^CTAGG BlpI 244 GCTCAGC GC^T[ACGT]AGC Bpu1102I 244 GCTCAGC GC^T[ACGT]AGC BsaAI 267 CACGTG [CT]AC^GT[GA] BsaHI 1946 GACGCC G[GA]^CG[CT]C BsoBI 305 CCCGGG C^[CT]CG[GA]G BspLU11I 3730 ACATGT A^CATGT BsrFI 542 GCCGGT [GA]^CCGG[CT] CelII 244 GCTCAGC GC^T[ACGT]AGC CfoI 116 GCGC GCG^C Cfr10I 542 GCCGGT [GA]^CCGG[CT] DraIII 267 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1675 CCACGG C^C[GA][CT]GG EarI 322 CTCTTC CTCTTC(1/4) HgaI 1946 GACGC GACGC(5/10) HhaI 116 GCGC GCG^C Hin1I 1946 GACGCC G[GA]^CG[CT]C HinP1I 116 GCGC G^CGC HindIII 1669 AAGCTT A^AGCTT HpaI 502 GTTAAC GTT^AAC KpnI 2864 GGTACC GGTAC^C Ksp632I 322 CTCTTC CTCTTC(1/4) MfeI 1140 CAATTG C^AATTG MunI 1140 CAATTG C^AATTG PmaCI 267 CACGTG CAC^GTG PmlI 267 CACGTG CAC^GTG PpuMI 3736 GGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 792 AACGTT AA^CGTT SacI 2443 GAGCTC GAGCT^C SbfI 342 CCTGCAGG CCTGCA^GG SmaI 305 CCCGGG CCC^GGG SphI 3633 GCATGC GCATG^C Sse8387I 342 CCTGCAGG CCTGCA^GG XbaI 574 TCTAGA T^CTAGA XcmI 3291 CCACTGTTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 305 CCCGGG C^CCGGG XmaI 305 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 209 TCCGGA T^CCGGA 3448 TCCGGA AlwNI 3686 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 3941 CAGACACTG AocI 2082 CCTAAGG CC^T[ACGT]AGG 2566 CCTGAGG BanI 2679 GGCACC G^G[CT][GA]CC 2864 GGTACC BbsI 352 GTCTTC GAAGAC(2/6) 1171 GTCTTC BclI 3911 TGATCA T^GATCA 4002 TGATCA BpuAI 352 GTCTTC GAAGAC(2/6) 1171 GTCTTC BseAI 209 TCCGGA T^CCGGA 3448 TCCGGA Bsp1407I 1775 TGTACA T^GTACA 4136 TGTACA BspEI 209 TCCGGA T^CCGGA 3448 TCCGGA BsrDI 2713 CATTGC GCAATG(2/0) 702 GCAATG BsrGI 1775 TGTACA T^GTACA 4136 TGTACA BstXI 2049 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2611 CCATCCAACTGG BstYI 527 AGATCT [GA]^GATC[CT] 2542 AGATCC Bsu36I 2082 CCTAAGG CC^T[ACGT]AGG 2566 CCTGAGG DraI 232 TTTAAA TTT^AAA 3425 TTTAAA Eco81I 2082 CCTAAGG CC^T[ACGT]AGG 2566 CCTGAGG FauI 59 CCCGC CCCGC(4/6) 2480 GCGGG FbaI 3911 TGATCA T^GATCA 4002 TGATCA MaeII 268 ACGT A^CGT 793 ACGT MflI 527 AGATCT [GA]^GATC[CT] 2542 AGATCC MroI 209 TCCGGA T^CCGGA 3448 TCCGGA NdeI 726 CATATG CA^TATG 4250 CATATG PshAI 1922 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2398 GACCTGGGTC SexAI 2201 ACCTGGT A^CC[AT]GGT 2860 ACCTGGT SspBI 1775 TGTACA T^GTACA 4136 TGTACA XhoII 527 AGATCT [GA]^GATC[CT] 2542 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BalI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmnI