##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Aor51HI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BciVI BclI
BfrI BglI BglII BlnI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstUI Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 2544 AACGTT AA^CGTT
Alw44I 1536 GTGCAC G^TGCAC
AocI 4036 CCTGAGG CC^T[ACGT]AGG
ApaI 1168 GGGCCC GGGCC^C
ApaLI 1536 GTGCAC G^TGCAC
Asp700I 1668 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2292 CACGTG CAC^GTG
BcgI 4160 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 394 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 394 GCTCAGC GC^T[ACGT]AGC
BsaAI 2292 CACGTG [CT]AC^GT[GA]
BsaBI 1800 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1689 TCATGA T^CATGA
BstEII 3418 GGTGACC G^GT[ACGT]ACC
BstPI 3418 GGTGACC G^GT[ACGT]ACC
BstYI 750 AGATCC [GA]^GATC[CT]
Bsu36I 4036 CCTGAGG CC^T[ACGT]AGG
CelII 394 GCTCAGC GC^T[ACGT]AGC
CfoI 3979 GCGC GCG^C
DraIII 772 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4036 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3418 GGTGACC G^GT[ACGT]ACC
EcoRI 1668 GAATTC G^AATTC
EcoRV 3906 GATATC GAT^ATC
FauI 4123 GCGGG CCCGC(4/6)
HgaI 3851 GCGTC GACGC(5/10)
HhaI 3979 GCGC GCG^C
HinP1I 3979 GCGC G^CGC
HindIII 796 AAGCTT A^AGCTT
MamI 1800 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1584 CAATTG C^AATTG
MflI 750 AGATCC [GA]^GATC[CT]
MunI 1584 CAATTG C^AATTG
PflMI 1547 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2292 CACGTG CAC^GTG
PmlI 2292 CACGTG CAC^GTG
PpuMI 1556 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2544 AACGTT AA^CGTT
RcaI 1689 TCATGA T^CATGA
SapI 1360 GCTCTTC GCTCTTC(1/4)
SexAI 470 ACCTGGT A^CC[AT]GGT
SspI 3844 AATATT AAT^ATT
SwaI 1072 ATTTAAAT ATTT^AAAT
Van91I 1547 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 342 CCAAAGTGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 750 AGATCC [GA]^GATC[CT]
XmnI 1668 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1193 AAATTC [GA]^AATT[CT]
1668 GAATTC
ApoI 1193 AAATTC [GA]^AATT[CT]
1668 GAATTC
AseI 655 ATTAAT AT^TAAT
1068 ATTAAT
AsnI 655 ATTAAT AT^TAAT
1068 ATTAAT
BanI 2856 GGCACC G^G[CT][GA]CC
4126 GGTGCC
BbsI 3821 GAAGAC GAAGAC(2/6)
2280 GTCTTC
BcnI 3006 CCCGG CC^[GC]GG
3259 CCGGG
BpuAI 3821 GAAGAC GAAGAC(2/6)
2280 GTCTTC
BsgI 3896 CTGCAC GTGCAG(16/14)
2766 GTGCAG
Bst1107I 2536 GTATAC GTA^TAC
3550 GTATAC
BstZ17I 2536 GTATAC GTA^TAC
3550 GTATAC
EcoT14I 1355 CCTTGG C^C[AT][AT]GG
2961 CCTTGG
HapII 3007 CCGG C^CGG
3259 CCGG
HpaII 3007 CCGG C^CGG
3259 CCGG
MspI 3007 CCGG C^CGG
3259 CCGG
NciI 3006 CCCGG CC^[GC]GG
3259 CCGGG
PshBI 655 ATTAAT AT^TAAT
1068 ATTAAT
PstI 977 CTGCAG CTGCA^G
3271 CTGCAG
SacI 923 GAGCTC GAGCT^C
2025 GAGCTC
StuI 1352 AGGCCT AGG^CCT
1900 AGGCCT
StyI 1355 CCTTGG C^C[AT][AT]GG
2961 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BalI
BanII BbvCI BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I