##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BamHI BbrPI BcgI BciVI BglII
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1595 GTAGAC GT^[AC][GT]AC
AclI 2511 AACGTT AA^CGTT
AflII 979 CTTAAG C^TTAAG
AflIII 2665 ACGTGT A^C[GA][CT]GT
AspI 4304 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1511 CCTAGG C^CTAGG
BbeI 4185 GGCGCC GGCGC^C
BclI 3483 TGATCA T^GATCA
BfrI 979 CTTAAG C^TTAAG
BlnI 1511 CCTAGG C^CTAGG
BlpI 1463 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1463 GCTGAGC GC^T[ACGT]AGC
BsaI 1951 GGTCTC GGTCTC(1/5)
BsrFI 110 ACCGGC [GA]^CCGG[CT]
BssSI 3581 CTCGTG CACGAG(-5/-1)
CelII 1463 GCTGAGC GC^T[ACGT]AGC
Cfr10I 110 ACCGGC [GA]^CCGG[CT]
DraI 2878 TTTAAA TTT^AAA
DraIII 463 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1598 GACCTAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 300 CCCGC CCCGC(4/6)
FbaI 3483 TGATCA T^GATCA
HaeII 4185 GGCGCC [GA]GCGC^[CT]
KasI 4185 GGCGCC G^GCGCC
KspI 2229 CCGCGG CCGC^GG
NarI 4185 GGCGCC GG^CGCC
NruI 3571 TCGCGA TCG^CGA
PflFI 4304 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3228 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2511 AACGTT AA^CGTT
SacII 2229 CCGCGG CCGC^GG
SbfI 286 CCTGCAGG CCTGCA^GG
ScaI 1339 AGTACT AGT^ACT
SexAI 159 ACCTGGT A^CC[AT]GGT
SfoI 4185 GGCGCC GGC^GCC
SmaI 1647 CCCGGG CCC^GGG
Sse8387I 286 CCTGCAGG CCTGCA^GG
Tth111I 4304 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1647 CCCGGG C^CCGGG
XmaI 1647 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 837 GGCGTC G[GA]^CG[CT]C
4185 GGCGCC
Alw44I 1140 GTGCAC G^TGCAC
3852 GTGCAC
ApaLI 1140 GTGCAC G^TGCAC
3852 GTGCAC
AvaI 1647 CCCGGG C^[CT]CG[GA]G
3202 CCCGAG
BalI 2005 TGGCCA TGG^CCA
4123 TGGCCA
BbsI 3225 GAAGAC GAAGAC(2/6)
1607 GTCTTC
BpuAI 3225 GAAGAC GAAGAC(2/6)
1607 GTCTTC
BsaBI 2301 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2675 GATGTCGATC
BsaHI 837 GGCGTC G[GA]^CG[CT]C
4185 GGCGCC
BsoBI 1647 CCCGGG C^[CT]CG[GA]G
3202 CCCGAG
BsrDI 827 GCAATG GCAATG(2/0)
1554 GCAATG
BstAPI 1800 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2630 GCATCTGCTGC
BstEII 209 GGTCACC G^GT[ACGT]ACC
2595 GGTGACC
BstPI 209 GGTCACC G^GT[ACGT]ACC
2595 GGTGACC
BstYI 95 AGATCC [GA]^GATC[CT]
1651 GGATCT
EcoO65I 209 GGTCACC G^GT[ACGT]ACC
2595 GGTGACC
Hin1I 837 GGCGTC G[GA]^CG[CT]C
4185 GGCGCC
MamI 2301 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2675 GATGTCGATC
MfeI 563 CAATTG C^AATTG
2196 CAATTG
MflI 95 AGATCC [GA]^GATC[CT]
1651 GGATCT
MluNI 2005 TGGCCA TGG^CCA
4123 TGGCCA
MscI 2005 TGGCCA TGG^CCA
4123 TGGCCA
MunI 563 CAATTG C^AATTG
2196 CAATTG
NcoI 214 CCATGG C^CATGG
2095 CCATGG
SphI 1800 GCATGC GCATG^C
3839 GCATGC
XhoII 95 AGATCC [GA]^GATC[CT]
1651 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I Asp718I AspHI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmnI