##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AviII
BbeI BbrPI BciVI BfrI BglII
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 1429 GGGCCC GGGCC^C
AvrII 3271 CCTAGG C^CTAGG
BlnI 3271 CCTAGG C^CTAGG
BsiEI 432 CGGCCG CG[GA][CT]^CG
BspHI 299 TCATGA T^CATGA
BsrBI 1194 GAGCGG CCGCTC(-3/-3)
BstAPI 3362 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 413 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 432 CGGCCG C^GGCCG
EclXI 432 CGGCCG C^GGCCG
Eco52I 432 CGGCCG C^GGCCG
HincII 605 GTTGAC GT[CT]^[GA]AC
HindII 605 GTTGAC GT[CT]^[GA]AC
KspI 2928 CCGCGG CCGC^GG
NcoI 1376 CCATGG C^CATGG
NdeI 2812 CATATG CA^TATG
RcaI 299 TCATGA T^CATGA
SacII 2928 CCGCGG CCGC^GG
SapI 536 GAAGAGC GCTCTTC(1/4)
SphI 120 GCATGC GCATG^C
XbaI 532 TCTAGA T^CTAGA
XcmI 4149 CCAATGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 3426 GGCGTC G[GA]^CG[CT]C
4654 GACGCC
AfaI 1119 GTAC GT^AC
1790 GTAC
Aor51HI 890 AGCGCT AGC^GCT
2347 AGCGCT
Asp700I 804 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1107 GAAAAAGTTC
AspI 1862 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACAGGGTC
BamHI 1247 GGATCC G^GATCC
1330 GGATCC
BclI 3243 TGATCA T^GATCA
3653 TGATCA
BsaHI 3426 GGCGTC G[GA]^CG[CT]C
4654 GACGCC
BssSI 32 CTCGTG CACGAG(-5/-1)
681 CTCGTG
BstEII 348 GGTGACC G^GT[ACGT]ACC
1686 GGTGACC
BstPI 348 GGTGACC G^GT[ACGT]ACC
1686 GGTGACC
Eco47III 890 AGCGCT AGC^GCT
2347 AGCGCT
EcoNI 1729 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3032 CCTTCTGCAGG
EcoO65I 348 GGTGACC G^GT[ACGT]ACC
1686 GGTGACC
FbaI 3243 TGATCA T^GATCA
3653 TGATCA
Hin1I 3426 GGCGTC G[GA]^CG[CT]C
4654 GACGCC
NaeI 430 GCCGGC GCC^GGC
3956 GCCGGC
NgoMIV 430 GCCGGC G^CCGGC
3956 GCCGGC
PaeR7I 1310 CTCGAG C^TCGAG
4555 CTCGAG
PflFI 1862 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACAGGGTC
RsaI 1119 GTAC GT^AC
1790 GTAC
SfaNI 443 GCATC GCATC(5/9)
4113 GCATC
SmaI 1157 CCCGGG CCC^GGG
2853 CCCGGG
Tth111I 1862 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3774 GACAGGGTC
XhoI 1310 CTCGAG C^TCGAG
4555 CTCGAG
XmaCI 1157 CCCGGG C^CCGGG
2853 CCCGGG
XmaI 1157 CCCGGG C^CCGGG
2853 CCCGGG
XmnI 804 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1107 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII