##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII BbeI BbrPI BcgI
BciVI BsaAI BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HaeII HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1727 GACGTC GACGT^C
AccII 469 CGCG CG^CG
AccIII 2796 TCCGGA T^CCGGA
AflII 61 CTTAAG C^TTAAG
Alw44I 1075 GTGCAC G^TGCAC
ApaLI 1075 GTGCAC G^TGCAC
Asp700I 3121 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4196 CCTAGG C^CTAGG
BbsI 1460 GTCTTC GAAGAC(2/6)
BclI 756 TGATCA T^GATCA
BfrI 61 CTTAAG C^TTAAG
BglII 682 AGATCT A^GATCT
BlnI 4196 CCTAGG C^CTAGG
BpuAI 1460 GTCTTC GAAGAC(2/6)
BsaBI 4284 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2796 TCCGGA [AT]^CCGG[AT]
BseAI 2796 TCCGGA T^CCGGA
BsmI 2578 GCATTC GAATGC(1/-1)
BspEI 2796 TCCGGA T^CCGGA
BsrDI 4520 CATTGC GCAATG(2/0)
BstEII 4237 GGTGACC G^GT[ACGT]ACC
BstPI 4237 GGTGACC G^GT[ACGT]ACC
BstUI 469 CGCG CG^CG
BstXI 1755 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 3194 GCGC GCG^C
EcoO65I 4237 GGTGACC G^GT[ACGT]ACC
EcoRI 1530 GAATTC G^AATTC
EcoRV 1846 GATATC GAT^ATC
FbaI 756 TGATCA T^GATCA
HhaI 3194 GCGC GCG^C
HinP1I 3194 GCGC G^CGC
HincII 1942 GTTGAC GT[CT]^[GA]AC
HindII 1942 GTTGAC GT[CT]^[GA]AC
KspI 468 CCGCGG CCGC^GG
MamI 4284 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2796 TCCGGA T^CCGGA
MseI 62 TTAA T^TAA
MvnI 469 CGCG CG^CG
NheI 800 GCTAGC G^CTAGC
SacII 468 CCGCGG CCGC^GG
SbfI 2771 CCTGCAGG CCTGCA^GG
SexAI 201 ACCAGGT A^CC[AT]GGT
Sse8387I 2771 CCTGCAGG CCTGCA^GG
Tru9I 62 TTAA T^TAA
XmnI 3121 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1530 GAATTC [GA]^AATT[CT]
1954 AAATTC
AcyI 1727 GACGTC G[GA]^CG[CT]C
1803 GACGCC
AflIII 497 ACATGT A^C[GA][CT]GT
3786 ACATGT
AocI 2322 CCTGAGG CC^T[ACGT]AGG
3307 CCTCAGG
ApoI 1530 GAATTC [GA]^AATT[CT]
1954 AAATTC
BsaHI 1727 GACGTC G[GA]^CG[CT]C
1803 GACGCC
BspHI 2850 TCATGA T^CATGA
3228 TCATGA
BspLU11I 497 ACATGT A^CATGT
3786 ACATGT
BssSI 1641 CACGAG CACGAG(-5/-1)
2446 CACGAG
Bsu36I 2322 CCTGAGG CC^T[ACGT]AGG
3307 CCTCAGG
DraIII 200 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4621 CACACTGTG
DrdI 3296 GACAACTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3526 GACAGGGTTGTC
Eco81I 2322 CCTGAGG CC^T[ACGT]AGG
3307 CCTCAGG
EcoNI 2989 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3418 CCTCTGTGAGG
EcoT22I 2878 ATGCAT ATGCA^T
3198 ATGCAT
Hin1I 1727 GACGTC G[GA]^CG[CT]C
1803 GACGCC
HindIII 1214 AAGCTT A^AGCTT
3233 AAGCTT
MaeII 1728 ACGT A^CGT
3948 ACGT
NaeI 4570 GCCGGC GCC^GGC
4684 GCCGGC
NgoMIV 4570 GCCGGC G^CCGGC
4684 GCCGGC
NsiI 2878 ATGCAT ATGCA^T
3198 ATGCAT
RcaI 2850 TCATGA T^CATGA
3228 TCATGA
SacI 6 GAGCTC GAGCT^C
1364 GAGCTC
SphI 3196 GCATGC GCATG^C
4128 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII