##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspI AviII BcgI BfrI BglII
BsaBI BsiWI BsmBI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HindIII HpaI KspI MamI MfeI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 321 GGTACC G^GTACC
AhdI 3964 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 321 GGTACC G^GTACC
AspEI 3964 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3723 CCTAGG C^CTAGG
BamHI 3248 GGATCC G^GATCC
BbeI 398 GGCGCC GGCGC^C
BbrPI 938 CACGTG CAC^GTG
BclI 3674 TGATCA T^GATCA
BlnI 3723 CCTAGG C^CTAGG
BspLU11I 2698 ACATGT A^CATGT
BsrDI 4260 CATTGC GCAATG(2/0)
Eam1105I 3964 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3663 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2102 GAATTC G^AATTC
EcoT22I 3918 ATGCAT ATGCA^T
FbaI 3674 TGATCA T^GATCA
KasI 398 GGCGCC G^GCGCC
KpnI 321 GGTACC GGTAC^C
MluI 775 ACGCGT A^CGCGT
NarI 398 GGCGCC GG^CGCC
NsiI 3918 ATGCAT ATGCA^T
PmaCI 938 CACGTG CAC^GTG
PmlI 938 CACGTG CAC^GTG
PshAI 2209 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 689 CCTGCAGG CCTGCA^GG
SexAI 2533 ACCTGGT A^CC[AT]GGT
SfoI 398 GGCGCC GGC^GCC
SphI 3735 GCATGC GCATG^C
Sse8387I 689 CCTGCAGG CCTGCA^GG
XbaI 1845 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1149 GTCGAC GT^[AC][GT]AC
1441 GTCGAC
AccIII 238 TCCGGA T^CCGGA
2756 TCCGGA
AcyI 398 GGCGCC G[GA]^CG[CT]C
1042 GACGCC
AvaI 1460 CTCGGG C^[CT]CG[GA]G
2310 CTCGGG
BciVI 1411 GTATCC GTATCC(6/5)
2142 GTATCC
BlpI 563 GCTGAGC GC^T[ACGT]AGC
977 GCTGAGC
Bpu1102I 563 GCTGAGC GC^T[ACGT]AGC
977 GCTGAGC
BsaAI 938 CACGTG [CT]AC^GT[GA]
2405 CACGTA
BsaHI 398 GGCGCC G[GA]^CG[CT]C
1042 GACGCC
BsaWI 238 TCCGGA [AT]^CCGG[AT]
2756 TCCGGA
BseAI 238 TCCGGA T^CCGGA
2756 TCCGGA
BsiEI 64 CGGTCG CG[GA][CT]^CG
331 CGGTCG
BsmI 3578 GAATGC GAATGC(1/-1)
3112 GCATTC
BsoBI 1460 CTCGGG C^[CT]CG[GA]G
2310 CTCGGG
BspEI 238 TCCGGA T^CCGGA
2756 TCCGGA
BspHI 580 TCATGA T^CATGA
3930 TCATGA
BsrFI 62 GCCGGT [GA]^CCGG[CT]
894 ACCGGC
BssSI 601 CACGAG CACGAG(-5/-1)
945 CTCGTG
CelII 563 GCTGAGC GC^T[ACGT]AGC
977 GCTGAGC
Cfr10I 62 GCCGGT [GA]^CCGG[CT]
894 ACCGGC
Hin1I 398 GGCGCC G[GA]^CG[CT]C
1042 GACGCC
MroI 238 TCCGGA T^CCGGA
2756 TCCGGA
MseI 1791 TTAA T^TAA
3981 TTAA
RcaI 580 TCATGA T^CATGA
3930 TCATGA
SalI 1149 GTCGAC G^TCGAC
1441 GTCGAC
SapI 2952 GAAGAGC GCTCTTC(1/4)
4102 GAAGAGC
SmlI 951 CTCAAG C^T[CT][GA]AG
4049 CTTGAG
StuI 2438 AGGCCT AGG^CCT
2642 AGGCCT
Tru9I 1791 TTAA T^TAA
3981 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII