##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI ApaI AscI AspEI AspI
AviII BamHI BbrPI BbvCI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII Bsu36I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRV EcoT22I
FseI FspI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmlI
Psp1406I PvuI RsrII SacI SalI
SexAI SfiI SmaI SnaBI SphI
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2969 GTATAC GT^[AC][GT]AC
AflIII 3957 ACATGT A^C[GA][CT]GT
Aor51HI 2109 AGCGCT AGC^GCT
AseI 4150 ATTAAT AT^TAAT
AsnI 4150 ATTAAT AT^TAAT
AvrII 2371 CCTAGG C^CTAGG
BanII 1074 GGGCTC G[GA]GC[CT]^C
BbeI 3163 GGCGCC GGCGC^C
BcgI 119 CGACATGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1009 AGATCT A^GATCT
BlnI 2371 CCTAGG C^CTAGG
BsaAI 1543 CACGTA [CT]AC^GT[GA]
BsaBI 1043 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2856 GTGCAG GTGCAG(16/14)
BsmI 455 GAATGC GAATGC(1/-1)
BspHI 661 TCATGA T^CATGA
BspLU11I 3957 ACATGT A^CATGT
BsrBI 1691 CCGCTC CCGCTC(-3/-3)
BsrDI 3192 CATTGC GCAATG(2/0)
BssSI 1626 CTCGTG CACGAG(-5/-1)
Bst1107I 2969 GTATAC GTA^TAC
BstAPI 374 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 879 TTCGAA TT^CGAA
BstEII 3373 GGTGACC G^GT[ACGT]ACC
BstPI 3373 GGTGACC G^GT[ACGT]ACC
BstZ17I 2969 GTATAC GTA^TAC
Eco47III 2109 AGCGCT AGC^GCT
EcoO65I 3373 GGTGACC G^GT[ACGT]ACC
KasI 3163 GGCGCC G^GCGCC
MamI 1043 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3163 GGCGCC GG^CGCC
NheI 3485 GCTAGC G^CTAGC
NspI 3957 ACATGT [GA]CATG^[CT]
NspV 879 TTCGAA TT^CGAA
PaeR7I 20 CTCGAG C^TCGAG
PmeI 3566 GTTTAAAC GTTT^AAAC
PshAI 1317 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4150 ATTAAT AT^TAAT
RcaI 661 TCATGA T^CATGA
SbfI 3579 CCTGCAGG CCTGCA^GG
ScaI 184 AGTACT AGT^ACT
SfoI 3163 GGCGCC GGC^GCC
SfuI 879 TTCGAA TT^CGAA
SgrAI 1089 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3852 ACTAGT A^CTAGT
Sse8387I 3579 CCTGCAGG CCTGCA^GG
StuI 3363 AGGCCT AGG^CCT
XbaI 3469 TCTAGA T^CTAGA
XhoI 20 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 3163 GGCGCC G[GA]^CG[CT]C
3207 GGCGTC
AflII 798 CTTAAG C^TTAAG
1846 CTTAAG
Alw44I 1675 GTGCAC G^TGCAC
1731 GTGCAC
ApaLI 1675 GTGCAC G^TGCAC
1731 GTGCAC
BalI 2067 TGGCCA TGG^CCA
3503 TGGCCA
BcnI 146 CCCGG CC^[GC]GG
152 CCCGG
BfrI 798 CTTAAG C^TTAAG
1846 CTTAAG
BglI 3356 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3496 GCCTGGGTGGC
BsaHI 3163 GGCGCC G[GA]^CG[CT]C
3207 GGCGTC
EcoRI 1246 GAATTC G^AATTC
1621 GAATTC
Hin1I 3163 GGCGCC G[GA]^CG[CT]C
3207 GGCGTC
MaeII 1544 ACGT A^CGT
3779 ACGT
MluNI 2067 TGGCCA TGG^CCA
3503 TGGCCA
MscI 2067 TGGCCA TGG^CCA
3503 TGGCCA
NaeI 173 GCCGGC GCC^GGC
1090 GCCGGC
NciI 146 CCCGG CC^[GC]GG
152 CCCGG
NcoI 1475 CCATGG C^CATGG
2746 CCATGG
NgoMIV 173 GCCGGC G^CCGGC
1090 GCCGGC
PpuMI 1601 AGGTCCT [GA]G^G[AT]CC[CT]
3070 GGGACCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
Asp718I AspHI AvaI AvaII BanI
BbsI BbvI BciVI BclI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HpaII HphI ItaI KpnI
Ksp632I KspI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI