##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI BciVI BclI
BfrI BglII BsaBI BsiWI BspDI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoRV
FbaI FseI HincII HindII HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspI SwaI TfiI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1156 GGTACC G^GTACC
AccIII 745 TCCGGA T^CCGGA
AcsI 2392 GAATTC [GA]^AATT[CT]
ApoI 2392 GAATTC [GA]^AATT[CT]
Asp700I 3234 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1156 GGTACC G^GTACC
AviII 3445 TGCGCA TGC^GCA
AvrII 1278 CCTAGG C^CTAGG
BbeI 437 GGCGCC GGCGC^C
BlnI 1278 CCTAGG C^CTAGG
BsaWI 745 TCCGGA [AT]^CCGG[AT]
BseAI 745 TCCGGA T^CCGGA
BsmI 1251 GAATGC GAATGC(1/-1)
Bsp1407I 1323 TGTACA T^GTACA
BspEI 745 TCCGGA T^CCGGA
BspHI 2969 TCATGA T^CATGA
BsrBI 1377 GAGCGG CCGCTC(-3/-3)
BsrDI 3119 CATTGC GCAATG(2/0)
BsrGI 1323 TGTACA T^GTACA
BssHII 804 GCGCGC G^CGCGC
BssSI 3805 CTCGTG CACGAG(-5/-1)
BstEII 394 GGTGACC G^GT[ACGT]ACC
BstPI 394 GGTGACC G^GT[ACGT]ACC
DrdI 3769 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 768 CGGCCG C^GGCCG
EclXI 768 CGGCCG C^GGCCG
Eco52I 768 CGGCCG C^GGCCG
EcoO65I 394 GGTGACC G^GT[ACGT]ACC
EcoRI 2392 GAATTC G^AATTC
EcoT22I 1253 ATGCAT ATGCA^T
FspI 3445 TGCGCA TGC^GCA
KasI 437 GGCGCC G^GCGCC
KpnI 1156 GGTACC GGTAC^C
KspI 87 CCGCGG CCGC^GG
MroI 745 TCCGGA T^CCGGA
NaeI 1824 GCCGGC GCC^GGC
NarI 437 GGCGCC GG^CGCC
NgoMIV 1824 GCCGGC G^CCGGC
NsiI 1253 ATGCAT ATGCA^T
RcaI 2969 TCATGA T^CATGA
SacII 87 CCGCGG CCGC^GG
SfiI 3237 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 437 GGCGCC GGC^GCC
SphI 3582 GCATGC GCATG^C
SspBI 1323 TGTACA T^GTACA
XmnI 3234 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1210 GTAGAC GT^[AC][GT]AC
3634 GTAGAC
AspI 2042 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3059 GACCCTGTC
BbrPI 3919 CACGTG CAC^GTG
4210 CACGTG
BbsI 2658 GTCTTC GAAGAC(2/6)
3673 GTCTTC
BbvCI 3469 CCTCAGC CCTCAGC(-5/-2)
2976 GCTGAGG
BcgI 679 CGAGGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1639 GCACTCACCTCG
BlpI 2538 GCTGAGC GC^T[ACGT]AGC
3408 GCTGAGC
Bpu1102I 2538 GCTGAGC GC^T[ACGT]AGC
3408 GCTGAGC
BpuAI 2658 GTCTTC GAAGAC(2/6)
3673 GTCTTC
BsaAI 3919 CACGTG [CT]AC^GT[GA]
4210 CACGTG
BsiEI 768 CGGCCG CG[GA][CT]^CG
3963 CGACCG
BsmBI 1903 CGTCTC CGTCTC(1/5)
3187 CGTCTC
BsrFI 1824 GCCGGC [GA]^CCGG[CT]
2707 GCCGGT
CelII 2538 GCTGAGC GC^T[ACGT]AGC
3408 GCTGAGC
Cfr10I 1824 GCCGGC [GA]^CCGG[CT]
2707 GCCGGT
DraIII 1309 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3678 CACTGAGTG
EarI 4000 CTCTTC CTCTTC(1/4)
71 GAAGAG
Ksp632I 4000 CTCTTC CTCTTC(1/4)
71 GAAGAG
MseI 2734 TTAA T^TAA
4460 TTAA
NspI 3582 GCATGC [GA]CATG^[CT]
4244 ACATGC
PflFI 2042 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3059 GACCCTGTC
PmaCI 3919 CACGTG CAC^GTG
4210 CACGTG
PmlI 3919 CACGTG CAC^GTG
4210 CACGTG
SmaI 276 CCCGGG CCC^GGG
1341 CCCGGG
Tru9I 2734 TTAA T^TAA
4460 TTAA
Tth111I 2042 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3059 GACCCTGTC
XmaCI 276 CCCGGG C^CCGGG
1341 CCCGGG
XmaI 276 CCCGGG C^CCGGG
1341 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII