##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
AscI Asp700I Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BclI
BglII BlnI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRV FbaI
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 2770 AACGTT AA^CGTT
AcyI 4157 GGCGTC G[GA]^CG[CT]C
AflII 4075 CTTAAG C^TTAAG
Alw44I 2536 GTGCAC G^TGCAC
Aor51HI 1223 AGCGCT AGC^GCT
ApaLI 2536 GTGCAC G^TGCAC
AseI 1309 ATTAAT AT^TAAT
AsnI 1309 ATTAAT AT^TAAT
AspI 4488 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2300 GTCTTC GAAGAC(2/6)
BciVI 97 GGATAC GTATCC(6/5)
BfrI 4075 CTTAAG C^TTAAG
BglI 1662 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2300 GTCTTC GAAGAC(2/6)
BsaAI 3788 TACGTG [CT]AC^GT[GA]
BsaBI 4184 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4157 GGCGTC G[GA]^CG[CT]C
BsaI 4463 GAGACC GGTCTC(1/5)
Bsp1407I 4363 TGTACA T^GTACA
BsrFI 201 GCCGGT [GA]^CCGG[CT]
BsrGI 4363 TGTACA T^GTACA
Cfr10I 201 GCCGGT [GA]^CCGG[CT]
DraI 2849 TTTAAA TTT^AAA
DraIII 3405 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 47 GACTTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1223 AGCGCT AGC^GCT
EcoT22I 3758 ATGCAT ATGCA^T
Hin1I 4157 GGCGTC G[GA]^CG[CT]C
HindIII 2858 AAGCTT A^AGCTT
MamI 4184 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3734 GCTAGC G^CTAGC
NsiI 3758 ATGCAT ATGCA^T
PaeR7I 1613 CTCGAG C^TCGAG
PflFI 4488 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1309 ATTAAT AT^TAAT
Psp1406I 2770 AACGTT AA^CGTT
SbfI 132 CCTGCAGG CCTGCA^GG
Sse8387I 132 CCTGCAGG CCTGCA^GG
SspBI 4363 TGTACA T^GTACA
StuI 2081 AGGCCT AGG^CCT
Tth111I 4488 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1613 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1895 CGCG CG^CG
3270 CGCG
AcsI 1775 GAATTC [GA]^AATT[CT]
2623 GAATTC
AflIII 1376 ACATGT A^C[GA][CT]GT
3703 ACATGT
ApoI 1775 GAATTC [GA]^AATT[CT]
2623 GAATTC
BamHI 1301 GGATCC G^GATCC
1965 GGATCC
BlpI 423 GCTGAGC GC^T[ACGT]AGC
1817 GCTCAGC
Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC
1817 GCTCAGC
BspLU11I 1376 ACATGT A^CATGT
3703 ACATGT
BspMI 478 ACCTGC ACCTGC(4/8)
3530 ACCTGC
BstEII 2403 GGTCACC G^GT[ACGT]ACC
2467 GGTTACC
BstPI 2403 GGTCACC G^GT[ACGT]ACC
2467 GGTTACC
BstUI 1895 CGCG CG^CG
3270 CGCG
CelII 423 GCTGAGC GC^T[ACGT]AGC
1817 GCTCAGC
EcoO65I 2403 GGTCACC G^GT[ACGT]ACC
2467 GGTTACC
EcoRI 1775 GAATTC G^AATTC
2623 GAATTC
HaeII 1223 AGCGCT [GA]GCGC^[CT]
1451 AGCGCC
MvnI 1895 CGCG CG^CG
3270 CGCG
NcoI 1092 CCATGG C^CATGG
2181 CCATGG
SapI 2867 GAAGAGC GCTCTTC(1/4)
3251 GAAGAGC
SmaI 3066 CCCGGG CCC^GGG
4017 CCCGGG
TaqI 1614 TCGA T^CGA
2488 TCGA
TthHB8I 1614 TCGA T^CGA
2488 TCGA
XmaCI 3066 CCCGGG C^CCGGG
4017 CCCGGG
XmaI 3066 CCCGGG C^CCGGG
4017 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII