##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI Asp700I AviII BcgI
BciVI BfrI BglII BsaBI BsiWI
BsmBI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HindIII HpaI KspI
MamI MfeI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI SacII ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1303 GACGTC GACGT^C
AhdI 4162 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4162 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1846 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1081 CCCGGG C^[CT]CG[GA]G
AvrII 3921 CCTAGG C^CTAGG
BamHI 3446 GGATCC G^GATCC
BbeI 1762 GGCGCC GGCGC^C
BbsI 2422 GTCTTC GAAGAC(2/6)
BclI 3872 TGATCA T^GATCA
BlnI 3921 CCTAGG C^CTAGG
BpuAI 2422 GTCTTC GAAGAC(2/6)
BsoBI 1081 CCCGGG C^[CT]CG[GA]G
BspLU11I 2896 ACATGT A^CATGT
BsrBI 2482 CCGCTC CCGCTC(-3/-3)
CpoI 191 CGGACCG CG^G[AT]CCG
Eam1105I 4162 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2835 GAATTC G^AATTC
EcoT22I 4116 ATGCAT ATGCA^T
FbaI 3872 TGATCA T^GATCA
KasI 1762 GGCGCC G^GCGCC
MluI 2139 ACGCGT A^CGCGT
MseI 4179 TTAA T^TAA
NarI 1762 GGCGCC GG^CGCC
NsiI 4116 ATGCAT ATGCA^T
PflFI 1846 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 191 CGGACCG CG^G[AT]CCG
SbfI 2053 CCTGCAGG CCTGCA^GG
SfiI 276 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1762 GGCGCC GGC^GCC
SmaI 1081 CCCGGG CCC^GGG
SphI 3933 GCATGC GCATG^C
Sse8387I 2053 CCTGCAGG CCTGCA^GG
Tru9I 4179 TTAA T^TAA
Tth111I 1846 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1081 CCCGGG C^CCGGG
XmaI 1081 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1166 GGTACC G^GTACC
1685 GGTACC
AccI 621 GTCGAC GT^[AC][GT]AC
2513 GTCGAC
AccIII 1602 TCCGGA T^CCGGA
2954 TCCGGA
AcsI 2835 GAATTC [GA]^AATT[CT]
3383 GAATTT
ApaI 56 GGGCCC GGGCC^C
1257 GGGCCC
ApoI 2835 GAATTC [GA]^AATT[CT]
3383 GAATTT
Asp718I 1166 GGTACC G^GTACC
1685 GGTACC
BbrPI 739 CACGTG CAC^GTG
2302 CACGTG
BsaAI 739 CACGTG [CT]AC^GT[GA]
2302 CACGTG
BsaWI 1602 TCCGGA [AT]^CCGG[AT]
2954 TCCGGA
BseAI 1602 TCCGGA T^CCGGA
2954 TCCGGA
BsiEI 1428 CGGTCG CG[GA][CT]^CG
1695 CGGTCG
BsmI 3776 GAATGC GAATGC(1/-1)
3310 GCATTC
BspEI 1602 TCCGGA T^CCGGA
2954 TCCGGA
BspHI 1944 TCATGA T^CATGA
4128 TCATGA
BsrDI 679 CATTGC GCAATG(2/0)
4457 CATTGC
BsrFI 1426 GCCGGT [GA]^CCGG[CT]
2258 ACCGGC
BstAPI 2043 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2703 GCACCGCCTGC
Cfr10I 1426 GCCGGT [GA]^CCGG[CT]
2258 ACCGGC
KpnI 1166 GGTACC GGTAC^C
1685 GGTACC
MroI 1602 TCCGGA T^CCGGA
2954 TCCGGA
PmaCI 739 CACGTG CAC^GTG
2302 CACGTG
PmlI 739 CACGTG CAC^GTG
2302 CACGTG
RcaI 1944 TCATGA T^CATGA
4128 TCATGA
SalI 621 GTCGAC G^TCGAC
2513 GTCGAC
SapI 3150 GAAGAGC GCTCTTC(1/4)
4300 GAAGAGC
StuI 1007 AGGCCT AGG^CCT
1028 AGGCCT
TfiI 2974 GAATC G^A[AT]TC
4221 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII