##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI AviII AvrII BbeI BbsI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsiWI BspDI BspLU11I BssHII
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
EclXI Eco52I EcoRI EcoT22I FseI
FspI HpaI KasI KspI MamI
MfeI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3741 GACGTC GACGT^C
AhdI 1809 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3409 GTGCAC G^TGCAC
ApaLI 3409 GTGCAC G^TGCAC
AspEI 1809 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3889 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2227 GGATCC G^GATCC
BcgI 3225 CGAAAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4222 GTATCC GTATCC(6/5)
BclI 938 TGATCA T^GATCA
BglII 3167 AGATCT A^GATCT
BsiEI 4023 CGATCG CG[GA][CT]^CG
BsmBI 932 CGTCTC CGTCTC(1/5)
Bsp1407I 3092 TGTACA T^GTACA
BspHI 1256 TCATGA T^CATGA
BsrBI 3585 GAGCGG CCGCTC(-3/-3)
BsrGI 3092 TGTACA T^GTACA
BssSI 3412 CACGAG CACGAG(-5/-1)
BstEII 2498 GGTCACC G^GT[ACGT]ACC
BstPI 2498 GGTCACC G^GT[ACGT]ACC
DraIII 631 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1809 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2498 GGTCACC G^GT[ACGT]ACC
EcoRV 1938 GATATC GAT^ATC
FbaI 938 TGATCA T^GATCA
HincII 4321 GTCAAC GT[CT]^[GA]AC
HindII 4321 GTCAAC GT[CT]^[GA]AC
MluI 4 ACGCGT A^CGCGT
NspI 3659 GCATGC [GA]CATG^[CT]
PaeR7I 3901 CTCGAG C^TCGAG
PflFI 3889 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1152 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4023 CGATCG CGAT^CG
RcaI 1256 TCATGA T^CATGA
SapI 3613 GAAGAGC GCTCTTC(1/4)
SphI 3659 GCATGC GCATG^C
SspBI 3092 TGTACA T^GTACA
Tth111I 3889 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3901 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2517 GTAGAC GT^[AC][GT]AC
4407 GTAGAC
AccII 5 CGCG CG^CG
3714 CGCG
AccIII 178 TCCGGA T^CCGGA
408 TCCGGA
AcsI 3751 AAATTT [GA]^AATT[CT]
4031 GAATTT
AcyI 2720 GACGCC G[GA]^CG[CT]C
3741 GACGTC
AflIII 4 ACGCGT A^C[GA][CT]GT
2543 ACACGT
Aor51HI 1857 AGCGCT AGC^GCT
1999 AGCGCT
ApoI 3751 AAATTT [GA]^AATT[CT]
4031 GAATTT
Asp700I 2766 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4031 GAATTTCTTC
BbrPI 1701 CACGTG CAC^GTG
2544 CACGTG
BsaHI 2720 GACGCC G[GA]^CG[CT]C
3741 GACGTC
BsaI 3127 GAGACC GGTCTC(1/5)
3199 GAGACC
BseAI 178 TCCGGA T^CCGGA
408 TCCGGA
BspEI 178 TCCGGA T^CCGGA
408 TCCGGA
BsrFI 2121 GCCGGC [GA]^CCGG[CT]
3663 GCCGGC
BstUI 5 CGCG CG^CG
3714 CGCG
Cfr10I 2121 GCCGGC [GA]^CCGG[CT]
3663 GCCGGC
Eco47III 1857 AGCGCT AGC^GCT
1999 AGCGCT
Hin1I 2720 GACGCC G[GA]^CG[CT]C
3741 GACGTC
HindIII 645 AAGCTT A^AGCTT
1790 AAGCTT
MroI 178 TCCGGA T^CCGGA
408 TCCGGA
MvnI 5 CGCG CG^CG
3714 CGCG
NaeI 2121 GCCGGC GCC^GGC
3663 GCCGGC
NcoI 2953 CCATGG C^CATGG
3538 CCATGG
NgoMIV 2121 GCCGGC G^CCGGC
3663 GCCGGC
PmaCI 1701 CACGTG CAC^GTG
2544 CACGTG
PmlI 1701 CACGTG CAC^GTG
2544 CACGTG
PpuMI 83 AGGACCT [GA]G^G[AT]CC[CT]
1372 GGGACCC
SbfI 1823 CCTGCAGG CCTGCA^GG
4129 CCTGCAGG
SexAI 479 ACCTGGT A^CC[AT]GGT
661 ACCTGGT
SfiI 834 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3632 GGCCTCCGTGGCC
SmaI 2761 CCCGGG CCC^GGG
3628 CCCGGG
Sse8387I 1823 CCTGCAGG CCTGCA^GG
4129 CCTGCAGG
XmaCI 2761 CCCGGG C^CCGGG
3628 CCCGGG
XmaI 2761 CCCGGG C^CCGGG
3628 CCCGGG
XmnI 2766 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4031 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII